Nori Human TERF1 ELISA Kit
Price range: $508.00 through $916.00
This ELISA kit is for quantification of TERF1 in human. This is a quick ELISA assay that reduces time to 50% compared to the conventional method, and the entire assay only takes 3 hours. This assay employs the quantitative sandwich enzyme immunoassay technique and uses biotin-streptavidin chemistry to improve the performance of the assays. An antibody specific for TERF1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TERF1 present is bound by the immobilized antibody. After washing away any unbound substances, a detection antibody specific for TERF1 is added to the wells. Following wash to remove any unbound antibody reagent, a detection reagent is added. After intensive wash a substrate solution is added to the wells and color develops in proportion to the amount of TERF1 bound in the initial step. The color development is stopped, and the intensity of the color is measured.
Alternative names for TERF1: Telomeric repeat-binding factor 1 , TRF1
This product is for Laboratory Research Use Only not for diagnostic and therapeutic purposes or any other purposes.
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Description
Nori Human TERF1 ELISA Kit Summary
Alternative names for TERF1: Telomeric repeat-binding factor 1 , TRF1
| Assay Type | Solid Phase Sandwich ELISA |
| Format | 96-well Microplate or 96-Well Strip Microplate |
| Method of Detection | Colorimetric |
| Number of Targets Detected | 1 |
| Target Antigen Accession Number |
P54274 |
| Assay Length | 3 hours |
| Quantitative/Semiquantitative | Quantitative |
| Sample Type | Plasma, Serum, Cell Culture, Urine, Cell/Tissue Lysates, Synovial Fluid, BAL, |
| Recommended Sample Dilution (Plasma/Serum) | No dilution for sample <ULOQ; sufficient dilution for samples >ULOQ |
| Sensitivity | 30 pg/mL |
| Detection Range | 0.156-10 ng/mL |
| Specificity | Human TERF1 |
| Cross-Reactivity | < 0.5% cross-reactivity observed with available related molecules, < 50% cross-species reactivity observed with species tested. |
| Interference | No significant interference observed with available related molecules |
| Storage/Stability | 4 ºC for up to 6 months |
| Usage | For Laboratory Research Use Only. Not for diagnostic or therapeutic use. |
| Additional Notes | The kit allows for use in multiple experiments. |
Standard Curve
Kit Components
1. Pre-coated 96-well Microplate
2. Biotinylated Detection Antibody
3. Streptavidin-HRP Conjugate
4. Lyophilized Standards
5. TMB One-Step Substrate
6. Stop Solution
7. 20 x PBS
8. Assay Buffer
Other Materials Required but not Provided:
1. Microplate Reader capable of measuring absorption at 450 nm
2. Log-log graph paper or computer and software for ELISA data analysis
3. Precision pipettes (1-1000 µl)
4. Multi-channel pipettes (300 µl)
5. Distilled or deionized water
Protocol Outline
1. Prepare all reagents, samples and standards as instructed in the datasheet.
2. Add 100 µl of Standard or samples to each well and incubate 1 h at RT.
3. Add 100 µl of Working Detection Antibody to each well and incubate 1 h at RT.
4. Add 100 µl of Working Streptavidin-HRP to each well and incubate 20 min at RT.
5. Add 100 µl of Substrate to each well and incubate 5-30 min at RT.
6. Add 50 µl of Stop Solution to each well and read at 450 nm immediately.
Background:
Telomeric repeat-binding factor 1 (TRF1 or TERF1) is encoded by the TERF1 gene[1] and contains a C-terminal Myb motif, a dimerization domain (TERF homology) near its N-terminus and an acidic N-terminus. TERF1 is present at telomeres throughout the cell cycle and functions as an inhibitor of telomerase, acting in cis to limit the elongation of individual chromosome ends.[2] It is known to protect telomeres in mammals from DNA mechanisms that are used for repair purposes and at the same time regulate the activity carried out by telomerase. TERF1 is a component of the shelterin nucleoprotein complex. The protein has the ultimate use of functioning as an inhibitor of telomerase. The protein acts as cis-regulatory elements in the process of limiting the ends of individual chromosomes from elongating as facilitated by telomerase and the TTAGGG sequences. TERF1 is also actively involved in biological processes such as the response to drug and the negative regulation of the maintenance of telomere through the process of semi-conservative replication, similar to that of cis. TERF1 is also involved in the biological processes of positive regulation of the polymerization of the microtubule and negative control of the process of DNA replication.[3] TERF1 aids the biological process of mitosis and is the positive regulation of mitosis. TERF 1 is also involved in the biological process of cell division and the negative regulation of the maintenance of telomere facilitated by the enzyme telomerase. TERF1 protein levels correlates with telomere length in colorectal cancer. Telomeres protect the chromosome from degradation by nucleases and end-to-end fusion. High levels of TRF1 were observed in 68.7% of tumor samples, while the majority of normal samples showed negative or weak TERF1 concentrations. Among the tumor samples, telomere length was significantly associated with TERF1 levels.
References
- Shen M, et al. (1998). Proc. Natl. Acad. Sci. U.S.A. 94 (25): 13618–23.
- Garton M, Laughton C (2013). Journal of Molecular Biology. 425 (16): 2910–21.
- Kaplan CW, Kitts CL (2003). Journal of Microbiological Methods. 54 (1): 121–5.
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