Nori Human Galectin-12 ELISA Kit
$461.00 – $832.00
This ELISA kit is for quantification of galectin-12 in human. This is a quick ELISA assay that reduces time to 50% compared to the conventional method, and the entire assay only takes 3 hours. This assay employs the quantitative sandwich enzyme immunoassay technique and uses biotin-streptavidin chemistry to improve the performance of the assays. An antibody specific for galectin-12 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any galectin-12 present is bound by the immobilized antibody. After washing away any unbound substances, a detection antibody specific for galectin-12 is added to the wells. Following wash to remove any unbound antibody reagent, a detection reagent is added. After intensive wash a substrate solution is added to the wells and color develops in proportion to the amount of galectin-12 bound in the initial step. The color development is stopped, and the intensity of the color is measured.
Alternative names for Galectin-12: Galectin 12, LGALS12, GRIP1
This product is for Laboratory Research Use Only not for diagnostic and therapeutic purposes or any other purposes.
- Description
- How Elisa Works
- Product Citation (0)
- Reviews (0)
Description
Nori Human Galectin-12 ELISA Kit Summary
Alternative names for Galectin-12: Galectin 12, LGALS12, GRIP1
| Assay Type | Solid Phase Sandwich ELISA |
| Format | 96-well Microplate or 96-Well Strip Microplate |
| Method of Detection | Colorimetric |
| Number of Targets Detected | 1 |
| Target Antigen Accession Number | Q96DT0 |
| Assay Length | 3 hours |
| Quantitative/Semiquantitative | Quantitative |
| Sample Type | Plasma, Serum, Cell Culture, Urine, Cell/Tissue Lysates, Synovial Fluid, BAL, |
| Recommended Sample Dilution (Plasma/Serum) | No dilution for sample <ULOQ; sufficient dilution for samples >ULOQ |
| Sensitivity | 60 pg/mL |
| Detection Range | 0.313-20 ng/mL |
| Specificity | Natural and recombinant human galectin-12 |
| Cross-Reactivity | < 0.5% cross-reactivity observed with available related molecules, < 50% cross-species reactivity observed with species tested. |
| Interference | No significant interference observed with available related molecules |
| Storage/Stability | 4 ºC for up to 6 months |
| Usage | For Laboratory Research Use Only. Not for diagnostic or therapeutic use. |
| Additional Notes | The kit allows for use in multiple experiments. |
Standard Curve
Kit Components
1. Pre-coated 96-well Microplate
2. Biotinylated Detection Antibody
3. Streptavidin-HRP Conjugate
4. Lyophilized Standards
5. TMB One-Step Substrate
6. Stop Solution
7. 20 x PBS
8. Assay Buffer
Other Materials Required but not Provided:
1. Microplate Reader capable of measuring absorption at 450 nm
2. Log-log graph paper or computer and software for ELISA data analysis
3. Precision pipettes (1-1000 µl)
4. Multi-channel pipettes (300 µl)
5. Distilled or deionized water
Protocol Outline
1. Prepare all reagents, samples and standards as instructed in the datasheet.
2. Add 100 µl of Standard or samples to each well and incubate 1 h at RT.
3. Add 100 µl of Working Detection Antibody to each well and incubate 1 h at RT.
4. Add 100 µl of Working Streptavidin-HRP to each well and incubate 20 min at RT.
5. Add 100 µl of Substrate to each well and incubate 5-30 min at RT.
6. Add 50 µl of Stop Solution to each well and read at 450 nm immediately.
Background:
Galectin 12 is encoded by galectin 12 gene and is a member of galectin super family [1]. Galectin-12 is an Adipose-expressed Galectin-like Molecule Possessing Apoptosis-inducing Activity [2]. Galectin-12 is required for adipogenic signaling and adipocyte Differentiation [3]. Overexpression of galectin-12 contributes to a differentiation block in acute promyelocytic leukemia cells, and suppression of galectin-12 facilitates granulocytic differentiation [4]. Lower LGALS12 gene expression is associated with acute myeloid leukemia [5]. Galectin-12 is silenced in colorectal cancer via hypermethylation of its promoter 6]. Ablation of Galectin-12 Inhibits Atherosclerosis through Enhancement of M2 Macrophage Polarization [7].
References
- Yang RY et al. (2001) J Biol Chem 276 (23), 20252-20260.
- Hotta K, et al. (2001) J Biol Chem 276 (36), 34089-34097.
- Yang RY et al. (2004) J Biol Chem 279 (28), 29761-29766.
- Xue H, et al. (2016) J Leukoc Biol 100 (4), 657-664.
- El Leithy AA, et al. (2015) Tumour Biol 36 (10), 7929-7939.
- Katzenmaier EM, et al. (2017) IUBMB Life 69 (12), 962-970.
- Lin ES, et al. (2020) Int J Mol Sci 21 (15), E5511.
Be the first to review “Nori Human Galectin-12 ELISA Kit”
You must be logged in to post a review.
Reviews
There are no reviews yet.