Nori Human Galectin-10 ELISA Kit Summary

Alternative names for Galectin-10: Galectin 10, LGALS10, LGALS10A, CLC

Standard Curve

https://genorise.com/wp-content/uploads/2026/06/Human-Galectin-10-Standard-Curve-1.jpg

Kit Components
1. Pre-coated 96-well Microplate
2. Biotinylated Detection Antibody
3. Streptavidin-HRP Conjugate
4. Lyophilized Standards
5. TMB One-Step Substrate
6. Stop Solution
7. 20 x PBS
8. Assay Buffer

Other Materials Required but not Provided:
1. Microplate Reader capable of measuring absorption at 450 nm
2. Log-log graph paper or computer and software for ELISA data analysis
3. Precision pipettes (1-1000 µl)
4. Multi-channel pipettes (300 µl)
5. Distilled or deionized water

Protocol Outline
1. Prepare all reagents, samples and standards as instructed in the datasheet.
2. Add 100 µl of Standard or samples to each well and incubate 1 h at RT.
3. Add 100 µl of Working Detection Antibody to each well and incubate 1 h at RT.
4. Add 100 µl of Working Streptavidin-HRP to each well and incubate 20 min at RT.
5. Add 100 µl of Substrate to each well and incubate 5-30 min at RT.
6. Add 50 µl of Stop Solution to each well and read at 450 nm immediately.

Background: 

Galectins are a class of proteins that bind specifically to β-galactoside sugars, such as N-acetyllactosamine (Galβ1-3GlcNAc or Galβ1-4GlcNAc), which can be bound to proteins by either N-linked or O-linked glycosylation. They are also termed S-type lectins due to their dependency on disulphide bonds for stability and carbohydrate binding. Galectin-10 is expressed in eosinophils and basophils and has essential role in immune system by suppression of T cell proliferation and regulates inflammation.[1] Galectins are distinct in that they can regulate cell death both intracellularly and extracellularly.[2] Extracellularly, they cross link glycans on the outside of cells and transduce signals across the membrane to directly cause cell death or activate downstream signaling that triggers apoptosis. Intracellularly, they can directly regulate proteins that control cell fate. Galectins can both promote and inhibit integrin-mediated adhesion. To enhance integrin-mediated adhesion, they cross link between two glycans on different cells. This brings the cells closer, so integrin binding occurs. They can also hinder adhesion by binding to two glycans on the same cell, which blocks the integrin^[3] binding site. Galectins are abundant, distributed widely around the body and have some distinct functions. Galectins are implicated in a wide range of diseases such as cancer, HIV, autoimmune disease, chronic inflammation, graft vs host disease and allergic reactions.[4]

References

1. Liu FT, Rabinovich GA (2010). Annals of the New York Academy of Sciences. 1183: 158–82.
2. Liu FT, et al. (2002). Biochimica et Biophysica Acta. 1572 (2–3): 263–73.
3. Thurston TL, et al. (2012). Nature. 482 (7385): 414–8.
4. Sato S, et al. (2012). Annals of the New York Academy of Sciences. 1253: 133–48.