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Recombinant Sheep IL-8 Protein

Placeholder Biotinylated Anti-Sheep TNFa Polyclonal Antibody
Placeholder Recombinant Sheep IL-10 Protein
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DataSheet   

The recombinant sheep IL-8 protein is derived from in vitro expression of sheep IL-8 gene in E. coli and purified using his-tag affinity column and can be used in multiple applications such as cell culture, ELISA and western blot.

Alternative names for IL-8: Interleukin 8, CXCL8

This product is for Laboratory Research Use Only not for diagnostic and therapeutic purposes or any other purposes.

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Description

Genorise Recombinant Sheep IL-8 Protein Summary

Alternative names for IL-8: Interleukin 8, CXCL8

Alternative name for sheep: ovine, lamb, goat

Product Specifications

Purity > 96%, by SDSPAGE under reducing conditions and visualized by silver stain.
Endotoxin Level < 0.1 EU per 1 μg of the protein by the LAL method.
Activity Measured by its ability to induce myeloperoxidase release from cytochalasin B-treated human neutrophils. JM Schroder et al (1987) J Immunol 139:3474.

The ED50 for this effect is 0.2-0.4 µg/ml.
Source E. coli derived sheep IL-8.
Accession # NP_001009401.1
N-Terminal Sequence Analysis Ser
Amino Acid Sequence Ser29-Pro101
Predicted Molecular Mass 8 kDa
SDS-PAGE 8 kDa, reducing conditions

 

Background: 

IL-8 was renamed CXCL8 by the Chemokine Nomenclature Subcommittee of the International Union of Immunological Societies (1), although its approved HUGO gene symbol remains IL8. CXCL8 was also referred to as neutrophil chemotactic factor (NCF), neutrophil activating protein (NAP), monocyte-derived neutrophil chemotactic factor (MDNCF), Tlymphocyte chemotactic factor (TCF), granulocyte chemotactic protein (GCP) and leukocyte adhesion inhibitor (LAI). Many cell types, including monocyte/macrophages, T cells, neutrophils, fibroblasts, endothelial cells, keratinocytes, hepatocytes, chondrocytes, and various tumor cell lines, can produce CXCL8 in response to a wide variety of proinflammatory stimuli such as exposure to IL1, TNF, LPS, and viruses. CXCL8 is a potent chemoattractant for neutrophils. In addition, CXCL8 also has a wide range of other proinflammatory effects. CXCL 8 is also a potent angiogenic factor. CXCL8 causes degranulation of neutrophil specific granules and azurophilic granules. CXCL8 induces expression of the cell adhesion molecules CD11/CD18 and enhances the adherence of neutrophils to endothelial cells and subendothelial matrix proteins. Besides neutrophils, CXCL8 is also chemotactic for basophils, T cells and eosinophils. CXCL8 has been reported to be a comitogen for keratinocytes and was also shown to be an autocrine growth factor for melanoma cells.  It is a key parameter in localized inflammation (2). It has been cited as a proinflammatory mediator in gingivitis and psoriasis (3).

References

  1. Bacon K, et al (October 2002). J. Interferon Cytokine Res. 22 (10): 1067–8.
  2. Vlahopoulos S, et al. (September 1999).  Blood 94 (6): 1878–89.
  3. Utgaard JO, et al (November 1998). Exp. Med. 188 (9): 1751–6.

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