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Recombinant Feline IL-13 Protein

Awaiting product image Recombinant Canine IL-13 Protein
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DataSheet   

The recombinant feline IL-13 protein is derived from in vivo expression of feline IL13 gene in E. coli and purified using his-tag affinity column and can be used in multiple applications such as cell culture.

Alternative names for IL-13: Interleukin 13

This product is for Laboratory Research Use Only, not for diagnostic and therapeutic purposes or any other purposes.

CAT: GR186046 Categories: , Tags: ,

Description

Genorise Recombinant Feline IL-13 Protein Summary

Alternative names for IL-13: Interleukin 13

Alternative name for feline: cat

Product Specifications

Purity > 97%, by SDSPAGE under reducing conditions and visualized by silver stain.
Endotoxin Level < 1.0 EU per 1 μg of the protein by the LAL method.
Activity Measured in a cell proliferation assay using TF1 human erythroleukemic cells.

Kitamura, T. et al. (1989) J. Cell Physiol. 140:323.

The ED50 for this effect is typically 1.6- 7.5 ng/mL.
Source E. coli derived feline IL-13.
Accession # A0ABI7ZMI3
N-Terminal Sequence Analysis Arg
Amino Acid Sequence Arg21-Lys141
Predicted Molecular Mass 13 kDa
SDS-PAGE 28 kDa, reducing conditions

 

Background: 

Interleukin 13 (IL-13) is a protein that is encoded by the IL13 gene.[1][2][3] IL-13 is cytokine secreted by many cell types, but especially T helper type 2 (Th2) cells,[4] that is a mediator of allergic inflammation and disease. In addition to effects on immune cells that are similar to those of the closely related cytokine IL-4, IL-13 is more importantly implicated as a central mediator of the physiologic changes induced by allergic inflammation in many tissues. IL-13 induces its effects through a multi-subunit receptor that includes the alpha chain of the IL-4 receptor (IL-4Rα) and at least one of two known IL-13-specific binding chains.[4] The functions of IL-13 overlap considerably with those of IL-4, especially with regard to changes induced on hematopoietic cells, but these effects are probably less important given the more potent role of IL-4. Thus, although IL-13 can induce immunoglobulin E (IgE) secretion from activated human B cells. Rather than a lymphoid cytokine, IL-13 acts more prominently as a molecular bridge linking allergic inflammatory cells to the non-immune cells in contact with them, thereby altering physiological function. Although IL-13 is associated primarily with the induction of airway disease, it also has anti-inflammatory properties. Airway matrix metalloproteinases (MMPs), which are protein-degrading enzymes, are required to induce egression of effete parenchymal inflammatory cells into the airway lumen where they are then cleared. Among other factors, IL-13 induces these MMPs as part of a mechanism that protects against excessive allergic inflammation that predisposes to asphyxiation.

Reference

  1. Minty A, et al. (1993). Nature 362 (6417): 248–50.
  2. McKenzie AN, et al. (1993) Natl. Acad. Sci. U.S.A. 90 (8): 3735–9.
  3. Morgan JG, et al.. Nucleic Acids Res. 20 (19): 5173–9.
  4. Wynn TA (2003). “IL-13 effector functions”. Rev. Immunol. 21: 425–56.
  5. Smith PC, et al (2001). Cytokine Growth Factor Rev. 12 (1): 33–40.
  6. Hong, D.S. et al. (2007) Cancer 110, 1911-28.
  7. Nishimoto N (2006). Curr Opin Rheumatol 18 (3): 277–81

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