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Recombinant Equine IL-1b Protein

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DataSheet   

The recombinant equine IL-1b protein is derived from in vitro expression of equine IL-1b gene in E. coli and purified using his-tag affinity column and can be used in multiple applications such as cell culture, ELISA and western blot.

Alternative names for IL-1b: Interleukin 1 beta, IL1B

This product is for Laboratory Research Use Only not for diagnostic and therapeutic purposes or any other purposes.

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Description

Genorise Recombinant Equine IL-1b Protein Summary

Alternative names for IL-1b: Interleukin IL-1 beta, IL1B

Alternative names for equine: horse

Product Specifications

Purity > 97%, by SDSPAGE under reducing conditions and visualized by silver stain.
Endotoxin Level < 0.1 EU per 1 μg of the protein by the LAL method.
Activity Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. Symons,

J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272.

The ED50 for this effect is typically 10-35 pg/ml.
Source E. coli derived equine IL-1B.
Accession # NP_001075995.1
N-Terminal Sequence Analysis Met
Amino Acid Sequence Met1-Ala268
Predicted Molecular Mass 30 kDa
SDS-PAGE 30 kDa, reducing conditions

 

Background: 

Interleukin-1 beta (IL-1β) also known as catabolin, is a member of the interleukin 1 cytokine family.  IL-1β precursor is cleaved by caspase 1 (interleukin 1 beta convertase). Cytosolic thiol protease cleaves the product to form mature IL-1β.  Interleukin-1 alpha and interleukin-1 beta forms IL-1 (1,2,3). IL-1β is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. IL-1β gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2 (4).

References

  1. Auron PE, et al (1984). Proc Natl Acad Sci U S A 81 (24): 7907–11.
  2. March CJ, et al. (1985). Nature 315 (6021): 641–7.
  3. Clark BD, et al (1986). Nucleic Acids Res 14 (20): 7897–1914.
  4. Entrez Gene: IL1B interleukin 1, beta. http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=3553

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