Awaiting product image
placeholder

Recombinant Canine IGF2 Protein

Awaiting product image Recombinant Canine BMP7 Protein
Awaiting product image Recombinant Canine Myeloperoxidase Protein
0 out of 5
( There are no reviews yet. )

Price range: $99.00 through $456.00

DataSheet   

The recombinant canine IGF2 protein is derived from in vitro expression of canine IGF2 gene in E. coli and purified using his-tag affinity column and can be used in multiple applications such as cell culture, ELISA and western blot.

Alternative names for IGF2: Insulin-like growth factor 2

This product is for Laboratory Research Use Only, not for diagnostic and therapeutic purposes or any other purposes.

CAT: GR122049 Categories: , Tag:

Description

Genorise Recombinant Canine IGF2 Protein Summary

Alternative names for IGF2: Insulin-like growth factor 2,

 

Product Specifications

Purity > 95%, by SDSPAGE under reducing conditions and visualized by silver stain.
Endotoxin Level < 0.1 EU per 1 μg of the protein by the LAL method.
Activity Measured in serum-free cell proliferation assay using MCF-7 human breast cancer cells. Karey KP et al. (1988) Cancer Res 48:4083. The ED50 for this effect is typically 2-7 ng/mL.
Source E. coli derived canine IGF2.
Accession # A0A8C0KV88
N-Terminal Sequence Analysis Pro30
Amino Acid Sequence Pro30 –Arg159
Predicted Molecular Mass 15 kDa
SDS-PAGE 30 kDa, reducing conditions

 

Background: 

Insulin-like growth factor 2 (IGF-2) is one of three protein hormones that share structural similarity to insulin. IGF-2 is a well-characterized neutral peptide believed to be secreted by the liver and to circulate in the blood. It has growth-regulating, insulin-like and mitogenic activities. It is believed to be a major fetal growth factor in contrast to Insulin-like growth factor 1, which is a major growth factor in adults”. The major role of IGF-2 is as a growth promoting hormone during gestation. IGF-2 exerts its effects by binding to the IGF-1 receptor. IGF2 may also bind to the IGF-2 receptor, which acts as a signaling antagonist; that is, to prevent IGF2 responses. In the process of folliculogenesis, IGF-2 is created by thecal cells to act in an autocrine manner on the theca cells themselves, and in a paracrine manner on granulosa cells in the ovary. IGF2 promotes granulosa cell proliferation during the follicular phase of the menstrual cycle, acting alongside follicle stimulating hormone (FSH). After ovulation has occurred, IGF-2 promotes progesterone secretion during the luteal phase of the menstrual cycle, together with luteinizing hormone (LH). Thus, IGF2 acts as a co-hormone together with both FSH and LH. IGF-2 may be linked to memory and reproduction.[1] Fear extinction-induced IGF2/IGFBP7 signaling promotes the survival of 17–19-day-old newborn hippocampal neurons. This suggests that therapeutic strategies that enhance IGF2 signaling and adult neurogenesis might be suitable to treat diseases linked to excessive fear memory such as PTSD.[2] Insulin-like growth factor 2 has been shown to interact with IGFBP3[3][4] and transferrin.[3]

References

  1. Chen DY, et al.(2011). Nature 469 (7331): 491–7.
  2. Agis-Balboa RC, et al. (2011). The EMBO Journal 30 (19): 4071–83.
  3. Storch S, et al. (2001). FEBS Letters 509 (3): 395–8.
  4. Buckway CK, et al. (2001). The Journal of Clinical Endocrinology and Metabolism 86 (10): 4943–50.

Product Citations

Reviews

There are no reviews yet.

Be the first to review “Recombinant Canine IGF2 Protein”