Nori Rat VEGF-B ELISA Kit
Price range: $508.00 through $916.00
This ELISA kit is for quantification of VEGF-B in rat. This is a quick ELISA assay that reduces time to 50% compared to the conventional method, and the entire assay only takes 3 hours. This assay employs the quantitative sandwich enzyme immunoassay technique and uses biotin-streptavidin chemistry to improve the performance of the assays. An antibody specific for VEGF-B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any VEGF-B present is bound by the immobilized antibody. After washing away any unbound substances, a detection antibody specific for VEGF-B is added to the wells. Following wash to remove any unbound antibody reagent, a detection reagent is added. After intensive wash a substrate solution is added to the wells and color develops in proportion to the amount of VEGF-B bound in the initial step. The color development is stopped, and the intensity of the color is measured.
Alternative names for VEGF-B: Vascular endothelial growth factor B, VEGF-related factor, VRF
This product is for laboratory research use only not for diagnostic and therapeutic purposes or any other purposes.
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Description
Nori Rat VEGF-B ELISA Kit Summary
Alternative names for VEGF-B: Vascular endothelial growth factor B, VEGF-related factor, VRF
| Assay Type | Solid Phase Sandwich ELISA |
| Format | 96-well Microplate or 96-Well Strip Microplate |
| Method of Detection | Colorimetric |
| Number of Targets Detected | 1 |
| Target Antigen Accession Number | O35485 |
| Assay Length | 3 hours |
| Quantitative/Semiquantitative | Quantitative |
| Sample Type | Plasma, Serum, Cell Culture, Urine, Cell/Tissue Lysates, Synovial Fluid, BAL, |
| Recommended Sample Dilution (Plasma/Serum) | No dilution for sample <ULOQ; sufficient dilution for samples >ULOQ |
| Sensitivity | 6 pg/mL |
| Detection Range | 31.25-2000 pg/mL |
| Specificity | Rat VEGF-B |
| Cross-Reactivity | < 0.5% cross-reactivity observed with available related molecules, < 50% cross-species reactivity observed with species tested. |
| Interference | No significant interference observed with available related molecules |
| Storage/Stability | 4 ºC for up to 6 months |
| Usage | For Laboratory Research Use Only. Not for diagnostic or therapeutic use. |
| Additional Notes | The kit allows for use in multiple experiments. |
Standard Curve
Kit Components
1. Pre-coated 96-well Microplate
2. Biotinylated Detection Antibody
3. Streptavidin-HRP Conjugate
4. Lyophilized Standards
5. TMB One-Step Substrate
6. Stop Solution
7. 20 x PBS
8. Assay Buffer
Other Materials Required but not Provided:
1. Microplate Reader capable of measuring absorption at 450 nm
2. Log-log graph paper or computer and software for ELISA data analysis
3. Precision pipettes (1-1000 µl)
4. Multi-channel pipettes (300 µl)
5. Distilled or deionized water
Protocol Outline
1. Prepare all reagents, samples and standards as instructed in the datasheet.
2. Add 100 µl of Standard or samples to each well and incubate 1 h at RT.
3. Add 100 µl of Working Detection Antibody to each well and incubate 1 h at RT.
4. Add 100 µl of Working Streptavidin-HRP to each well and incubate 20 min at RT.
5. Add 100 µl of Substrate to each well and incubate 5-30 min at RT.
6. Add 50 µl of Stop Solution to each well and read at 450 nm immediately.
Background:
Vascular endothelial growth factor B also known as VEGF-B is a protein that is encoded by the VEGF-B gene. VEGF-B is a growth factor that belongs to the vascular endothelial growth factor family. In contrast to VEGF-A, VEGF-B plays a less pronounced role in the vascular system: Whereas VEGF-A is important for the formation of blood vessels, such as during development or in pathological conditions, VEGF-B seems to play a role only in the maintenance of newly formed blood vessels during pathological conditions.[1] VEGF-B plays also an important role on several types of neurons. It is important for the protection of neurons in the retina[2] and the cerebral cortex during stroke [3] and of motoneurons during motor neuron diseases such as amyotrophic lateral sclerosis.[4] VEGF-B exerts its effects via the FLT1 receptor.[5] VEGF-B has also been found to control endothelial uptake and transport of fatty acids in heart and skeletal muscle.[6][7] VEGF-B has been shown to interact with FLT1.[8][9]
References
- Zhang F, et al. (2009). Proc. Natl. Acad. Sci. U.S.A. 106 (15): 6152–7.
- Li Y, et al. (2008). J. Clin. Invest. 118 (3): 913–23.
- Sun Y, et al. (2004). J. Cereb. Blood Flow Metab. 24 (10): 1146–52.
- Poesen K, et al. (2008). J. Neurosci. 28 (42): 10451–9.
- Yamazaki Y, Morita T (2006). Mol. Divers. 10 (4): 515–27.
- Muoio DM (2010). N. Engl. J. Med. 363 (3): 291–3.
- Hagberg CE, et al. (2012). Nature. 490 (7420): 426–30.
- Olofsson B, et al. (1998). Proc. Natl. Acad. Sci. U.S.A. 95 (20): 11709–14.
- Makinen T, et al. (1999). J. Biol. Chem. 274 (30): 21217–22.
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