Nori Rat MMP19 ELISA Kit
Price range: $508.00 through $916.00
This ELISA kit is for quantification of MMP19 in rat. This is a quick ELISA assay that reduces time to 50% compared to the conventional method, and the entire assay only takes 3 hours. This assay employs the quantitative sandwich enzyme immunoassay technique and uses biotin-streptavidin chemistry to improve the performance of the assays. An antibody specific for MMP19 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MMP19 present is bound by the immobilized antibody. After washing away any unbound substances, a detection antibody specific for MMP19 is added to the wells. Following wash to remove any unbound antibody reagent, a detection reagent is added. After intensive wash a substrate solution is added to the wells and color develops in proportion to the amount of MMP19 bound in the initial step. The color development is stopped, and the intensity of the color is measured.
Alternative names for MMP19: Matrix metalloproteinase-19, MMP-19, MMP18, RASI
This product is for Laboratory Research Use Only not for diagnostic and therapeutic purposes or any other purposes.
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Description
Nori Rat MMP19 ELISA Kit Summary
Alternative names for MMP19: Matrix metalloproteinase-19, MMP-19, RASI, MMP18
| Assay Type | Solid Phase Sandwich ELISA |
| Format | 96-well Microplate or 96-Well Strip Microplate |
| Method of Detection | Colorimetric |
| Number of Targets Detected | 1 |
| Target Antigen Accession Number | A6KKI7 |
| Assay Length | 3 hours |
| Quantitative/Semiquantitative | Quantitative |
| Sample Type | Plasma, Serum, Cell Culture, Urine, Cell/Tissue Lysates, Synovial Fluid, BAL, |
| Recommended Sample Dilution (Plasma/Serum) | No dilution for sample <ULOQ; sufficient dilution for samples >ULOQ |
| Sensitivity | 30 pg/mL |
| Detection Range | 0.156-10 ng/mL |
| Specificity | Rat MMP19 |
| Cross-Reactivity | < 0.5% cross-reactivity observed with available related molecules, < 50% cross-species reactivity observed with species tested. |
| Interference | No significant interference observed with available related molecules |
| Storage/Stability | 4 ºC for up to 6 months |
| Usage | For Laboratory Research Use Only. Not for diagnostic or therapeutic use. |
| Additional Notes | The kit allows for use in multiple experiments. |
Standard Curve
Kit Components
1. Pre-coated 96-well Microplate
2. Biotinylated Detection Antibody
3. Streptavidin-HRP Conjugate
4. Lyophilized Standards
5. TMB One-Step Substrate
6. Stop Solution
7. 20 x PBS
8. Assay Buffer
Other Materials Required but not Provided:
1. Microplate Reader capable of measuring absorption at 450 nm
2. Log-log graph paper or computer and software for ELISA data analysis
3. Precision pipettes (1-1000 µl)
4. Multi-channel pipettes (300 µl)
5. Distilled or deionized water
Protocol Outline
1. Prepare all reagents, samples and standards as instructed in the datasheet.
2. Add 100 µl of Standard or samples to each well and incubate 1 h at RT.
3. Add 100 µl of Working Detection Antibody to each well and incubate 1 h at RT.
4. Add 100 µl of Working Streptavidin-HRP to each well and incubate 20 min at RT.
5. Add 100 µl of Substrate to each well and incubate 5-30 min at RT.
6. Add 50 µl of Stop Solution to each well and read at 450 nm immediately.
Background:
Matrix metalloproteinase-19 (MMP-19) also known as matrix metalloproteinase RASI is an enzyme that is encoded by the MMP19 gene.[1] Multiple transcript variants encoding distinct isoforms have been identified for MMP-19 gene. Proteins of MMP family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP’s are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. MMP-19 is expressed in epidermis and endothelial cells and it has a role in cellular proliferation, migration, angiogenesis and adhesion. Matrix metalloproteinase MMP-19 (RASI-1) is expressed on the surface of activated peripheral blood mononuclear cells and is detected as an autoantigen in rheumatoid arthritis.[2] Matrix metalloproteinase-19 is expressed in myeloid cells in an adhesion-dependent manner and associates with the cell surface.[3] RASI-1 is expressed in synovial blood vessels of a rheumatoid arthritis patient.[4] MMP-19 expression is regulated by microRNA such as microRNA-193b-3p [5] and microRNA-16.[6] MMP-19 inhibits capillary-like formation due to processing of nidogen-1.[5] Study found that the expression of MMP19 was upregulated in colorectal cancer (CRC). High expression of MMP19 was determined to be an independent and poor prognostic factor in CRC, suggesting that MMP19 may be a good biomarker for CRC.[6]
References
- Kolb C, et al. (1997). Immunol Lett. 57 (1–3): 83–8.
- Sedlacek R, Mauch S, Kolb B, et al. (1998). Immunobiology. 198 (4): 408–23.
- Mauch S, Kolb C, Kolb B, et al. (2002). J. Immunol. 168 (3): 1244–51.
- Kolb C, et al. (1997) Immunol Lett 57 (1-3), 83-88.
- Chang ZK, et al. (2018) J Cell Biochem 119 (6), 4775-4782.
- Tian G, (2019) Eur Rev Med Pharmacol Sci 23 (12), 5260-5269.
- Titz B, et al. (2004). Cell Mol Life Sci. 61 (14): 1826–33.
- Chen Z, et al. (2019) BMC Cancer 19 (1), 448.
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