Nori Rat IL-7 ELISA Kit
Price range: $508.00 through $916.00
This ELISA kit is for quantification of IL-7 in rat. This is a quick ELISA assay that reduces time to 50% compared to the conventional method, and the entire assay only takes 3 hours. This assay employs the quantitative sandwich enzyme immunoassay technique and uses biotin-streptavidin chemistry to improve the performance of the assays. An antibody specific for IL-7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-7 present is bound by the immobilized antibody. After washing away any unbound substances, a detection antibody specific for IL-7 is added to the wells. Following wash to remove any unbound antibody reagent, a detection reagent is added. After intensive wash a substrate solution is added to the wells and color develops in proportion to the amount of IL-7 bound in the initial step. The color development is stopped, and the intensity of the color is measured.
Alternative names for IL-7: IL7, interleukin 7
This product is for laboratory research use only not for diagnostic and therapeutic purposes or any other purposes.
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Description
Nori Rat IL-7 ELISA Kit Summary
Alternative names for IL-7: IL7, interleukin 7
| Assay Type | Solid Phase Sandwich ELISA |
| Format | 96-well Microplate or 96-Well Strip Microplate |
| Method of Detection | Colorimetric |
| Number of Targets Detected | 1 |
| Target Antigen Accession Number | P56478 |
| Assay Length | 3 hours |
| Quantitative/Semiquantitative | Quantitative |
| Sample Type | Plasma, Serum, Cell Culture, Urine, Cell/Tissue Lysates, Synovial Fluid, BAL, |
| Recommended Sample Dilution (Plasma/Serum) | No dilution for sample <ULOQ; sufficient dilution for samples >ULOQ |
| Sensitivity | 3 pg/mL |
| Detection Range | 15.63-1000 pg/mL |
| Specificity | Rat IL-7 |
| Cross-Reactivity | < 0.5% cross-reactivity observed with available related molecules, < 50% cross-species reactivity observed with species tested. |
| Interference | No significant interference observed with available related molecules |
| Storage/Stability | 4 ºC for up to 6 months |
| Usage | For Laboratory Research Use Only. Not for diagnostic or therapeutic use. |
| Additional Notes | The kit allows for use in multiple experiments. |
Standard Curve
Kit Components
1. Pre-coated 96-well Microplate
2. Biotinylated Detection Antibody
3. Streptavidin-HRP Conjugate
4. Lyophilized Standards
5. TMB One-Step Substrate
6. Stop Solution
7. 20 x PBS
8. Assay Buffer
Other Materials Required but not Provided:
1. Microplate Reader capable of measuring absorption at 450 nm
2. Log-log graph paper or computer and software for ELISA data analysis
3. Precision pipettes (1-1000 µl)
4. Multi-channel pipettes (300 µl)
5. Distilled or deionized water
Protocol Outline
1. Prepare all reagents, samples and standards as instructed in the datasheet.
2. Add 100 µl of Standard or samples to each well and incubate 1 h at RT.
3. Add 100 µl of Working Detection Antibody to each well and incubate 1 h at RT.
4. Add 100 µl of Working Streptavidin-HRP to each well and incubate 20 min at RT.
5. Add 100 µl of Substrate to each well and incubate 5-30 min at RT.
6. Add 50 µl of Stop Solution to each well and read at 450 nm immediately.
Background:
IL-7 is a hematopoietic growth factor secreted by stromal cells in the bone marrow and thymus. It is also produced by keratinocytes,[1] dendritic cells,[2] hepatocytes,[3] neurons, and epithelial cells,[4] but is not produced by normal lymphocytes.[5] IL-7 stimulates the differentiation of multipotent (pluripotent) hematopoietic stem cells into lymphoid progenitor cells (as opposed to myeloid progenitor cells where differentiation is stimulated by IL-3). It also stimulates proliferation of all cells in the lymphoid lineage. It is important for proliferation during certain stages of B-cell maturation, T and NK cell survival, development and homeostasis. IL-7 is a cytokine important for B and T cell development. IL-7 and the hepatocyte growth factor (HGF) form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. This cytokine is found to be a cofactor for V(D)J rearrangement of the T cell receptor beta (TCRß) during early T cell development.[6] IL-7 can be produced locally by intestinal epithelial and epithelial goblet cells, and may serve as a regulatory factor for intestinal mucosal lymphocytes. Knockout studies in mice suggested that IL-7 plays an essential role in lymphoid cell survival. IL-7 binds to the IL-7 receptor, a heterodimer consisting of Interleukin-7 receptor alpha and common gamma chain receptor,[7] resulting in a cascade of signals important for T-cell development within the thymus and survival within the periphery. IL-7 promotes hematological malignancies (acute lymphoblastic leukemia, T cell lymphoma).[18]
References
- Heufler C, et al. (1993). J. Exp. Med. 178 (3): 1109–14.
- Kröncke R, et al. (1996). Eur. J. Immunol. 26 (10): 2541–4.
- Sawa Y, et al. (2009). Immunity. 30 (3): 447–57.
- Watanabe M, et al. (1995). J. Clin. Invest. 95 (6): 2945–53.
- Fry TJ, Mackall CL (2002). Blood. 99 (11): 3892–904.
- Muegge K, Vila MP, Durum SK (July 1993). “Interleukin-7: a cofactor for V(D)J rearrangement of the T cell receptor beta gene”. Science. 261 (5117): 93–5.
- Noguchi M, et al. (1994). Science. 262 (5141): 1877–80.
- Or R, et al. (1998). Cytokines Cell. Mol. Ther. 4 (4): 287–94.
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