Nori Mouse PECAM-1 ELISA Kit
Price range: $508.00 through $916.00
This ELISA kit is for quantification of PECAM-1 in mouse. This is a quick ELISA assay that reduces time to 50% compared to the conventional method, and the entire assay only takes 3 hours. This assay employs the quantitative sandwich enzyme immunoassay technique and uses biotin-streptavidin chemistry to improve the performance of the assays. An antibody specific for PECAM-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PECAM-1 present is bound by the immobilized antibody. After washing away any unbound substances, a detection antibody specific for PECAM-1 is added to the wells. Following wash to remove any unbound antibody reagent, a detection reagent is added. After intensive wash a substrate solution is added to the wells and color develops in proportion to the amount of PECAM-1 bound in the initial step. The color development is stopped, and the intensity of the color is measured.
Alternative names for PECAM-1: Platelet endothelial cell adhesion molecule, PECAM1, cluster of differentiation 31, CD31
This product is for Laboratory Research Use Only not for diagnostic and therapeutic purposes or any other purposes.
- Description
- How Elisa Works
- Documents
- Product Citations (0)
- Reviews (0)
Description
Nori Mouse PECAM-1 ELISA Kit Summary
Alternative names for PECAM-1: Platelet endothelial cell adhesion molecule, PECAM1, cluster of differentiation 31, CD31
| Assay Type | Solid Phase Sandwich ELISA |
| Format | 96-well Microplate or 96-Well Strip Microplate |
| Method of Detection | Colorimetric |
| Number of Targets Detected | 1 |
| Target Antigen Accession Number | Q08481 |
| Assay Length | 3 hours |
| Quantitative/Semiquantitative | Quantitative |
| Sample Type | Plasma, Serum, Cell Culture, Urine, Cell/Tissue Lysates, Synovial Fluid, BAL, |
| Recommended Sample Dilution (Plasma/Serum) | No dilution for sample <ULOQ; sufficient dilution for samples >ULOQ |
| Sensitivity | 60 pg/mL |
| Detection Range | 0.313-20 ng/mL |
| Specificity | Mouse PECAM-1 |
| Cross-Reactivity | < 0.5% cross-reactivity observed with available related molecules, < 50% cross-species reactivity observed with species tested. |
| Interference | No significant interference observed with available related molecules |
| Storage/Stability | 4 ºC for up to 6 months |
| Usage | For Laboratory Research Use Only. Not for diagnostic or therapeutic use. |
| Additional Notes | The kit allows for use in multiple experiments. |
Standard Curve
Kit Components
1. Pre-coated 96-well Microplate
2. Biotinylated Detection Antibody
3. Streptavidin-HRP Conjugate
4. Lyophilized Standards
5. TMB One-Step Substrate
6. Stop Solution
7. 20 x PBS
8. Assay Buffer
Other Materials Required but not Provided:
1. Microplate Reader capable of measuring absorption at 450 nm
2. Log-log graph paper or computer and software for ELISA data analysis
3. Precision pipettes (1-1000 µl)
4. Multi-channel pipettes (300 µl)
5. Distilled or deionized water
Protocol Outline
1. Prepare all reagents, samples and standards as instructed in the datasheet.
2. Add 100 µl of Standard or samples to each well and incubate 1 h at RT.
3. Add 100 µl of Working Detection Antibody to each well and incubate 1 h at RT.
4. Add 100 µl of Working Streptavidin-HRP to each well and incubate 20 min at RT.
5. Add 100 µl of Substrate to each well and incubate 5-30 min at RT.
6. Add 50 µl of Stop Solution to each well and read at 450 nm immediately.
Background:
Platelet endothelial cell adhesion molecule (PECAM-1) also known as cluster of differentiation 31 (CD31) is a protein that is encoded by the PECAM1 gene.[1][2] PECAM-1 plays a key role in removing aged neutrophils from the body. PECAM-1 is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. PECAM-1 is a member of the immunoglobulin superfamily and is likely involved in leukocyte transmigration, angiogenesis, and integrin activation. CD31 on endothelial cells binds to the CD38 receptor on natural killer cells for those cells to attach to the endothelium.[3][4] CD31 is normally found on endothelial cells, platelets, macrophages and Kupffer cells, granulocytes, lymphocytes, megakaryocytes, and osteoclasts. CD31 is also expressed in certain tumors, including epithelioid hemangioendothelioma, epithelioid sarcoma-like hemangioendothelioma, other vascular tumors, histiocytic malignancies, and plasmacytomas. It is rarely found in some sarcomas, such as Kaposi’s sarcoma, and carcinomas. In immunohistochemistry, CD31 is used primarily to demonstrate the presence of endothelial cells in histological tissue sections. This can help to evaluate the degree of tumor angiogenesis, which can imply a rapidly growing tumor. Malignant endothelial cells also commonly retain the antigen, so that CD31 immunohistochemistry can also be used to demonstrate both angiomas and angiosarcomas. It can also be demonstrated in small lymphocytic and lymphoblastic lymphomas, although more specific markers are available for these conditions.
References
- Newman PJ, et al. (1990). “PECAM-1 (CD31) cloning and relation to adhesion molecules of the immunoglobulin gene superfamily”. Science. 247 (4947): 1219–22.
- Albelda SM, et al. (1991) J Cell Biol 114 (5), 1059-1068
- Zambello R, Barilà G, Sabrina Manni S (2020). “NK cells and CD38: Implication for (Immuno)Therapy in Plasma Cell Dyscrasias”. Cells. 9 (3): 768.
- Glaría E, Valledor AF (2020). “Roles of CD38 in the Immune Response to Infection”. Cells. 9 (1): 228.
Product Citations
Be the first to review “Nori Mouse PECAM-1 ELISA Kit”
You must be logged in to post a review.
Reviews
There are no reviews yet.