Nori Mouse DLK1 ELISA Kit
Price range: $508.00 through $916.00
This ELISA kit is for quantification of DLK1 in mouse. This is a quick ELISA assay that reduces time to 50% compared to the conventional method, and the entire assay only takes 3 hours. This assay employs the quantitative sandwich enzyme immunoassay technique and uses biotin-streptavidin chemistry to improve the performance of the assays. An antibody specific for DLK1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DLK1 present is bound by the immobilized antibody. After washing away any unbound substances, a detection antibody specific for DEFA1 is added to the wells. Following wash to remove any unbound antibody reagent, a detection reagent is added. After intensive wash a substrate solution is added to the wells and color develops in proportion to the amount of DLK1 bound in the initial step. The color development is stopped, and the intensity of the color is measured.
Alternative names for DLK1: Protein delta homolog 1, delta-like 1 (DLK1) homolog decreases, Pref-1 (preadipocyte factor 1), fetal antigen 1 (FA1),
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Description
Nori Mouse DLK1 ELISA Kit Summary
Alternative names for DLK1: Protein delta homolog 1, delta-like 1 (DLK1) homolog decreases, Pref-1 (preadipocyte factor 1), fetal antigen 1 (FA1),
| Assay Type | Solid Phase Sandwich ELISA |
| Format | 96-well Microplate or 96-Well Strip Microplate |
| Method of Detection | Colorimetric |
| Number of Targets Detected | 1 |
| Target Antigen Accession Number |
Q09163 |
| Assay Length | 3 hours |
| Quantitative/Semiquantitative | Quantitative |
| Sample Type | Plasma, Serum, Cell Culture, Urine, Cell/Tissue Lysates, Synovial Fluid, BAL, |
| Recommended Sample Dilution (Plasma/Serum) | No dilution for sample <ULOQ; sufficient dilution for samples >ULOQ |
| Sensitivity | 25 pg/mL |
| Detection Range | 125-8000 pg/mL |
| Specificity | Mouse DLK1 |
| Cross-Reactivity | < 0.5% cross-reactivity observed with available related molecules, < 50% cross-species reactivity observed with species tested. |
| Interference | No significant interference observed with available related molecules |
| Storage/Stability | 4 ºC for up to 6 months |
| Usage | For Laboratory Research Use Only. Not for diagnostic or therapeutic use. |
| Additional Notes | The kit allows for use in multiple experiments. |
Standard Curve
Kit Components
1. Pre-coated 96-well Microplate
2. Biotinylated Detection Antibody
3. Streptavidin-HRP Conjugate
4. Lyophilized Standards
5. TMB One-Step Substrate
6. Stop Solution
7. 20 x PBS
8. Assay Buffer
Other Materials Required but not Provided:
1. Microplate Reader capable of measuring absorption at 450 nm
2. Log-log graph paper or computer and software for ELISA data analysis
3. Precision pipettes (1-1000 µl)
4. Multi-channel pipettes (300 µl)
5. Distilled or deionized water
Protocol Outline
1. Prepare all reagents, samples and standards as instructed in the datasheet.
2. Add 100 µl of Standard or samples to each well and incubate 1 h at RT.
3. Add 100 µl of Working Detection Antibody to each well and incubate 1 h at RT.
4. Add 100 µl of Working Streptavidin-HRP to each well and incubate 20 min at RT.
5. Add 100 µl of Substrate to each well and incubate 5-30 min at RT.
6. Add 50 µl of Stop Solution to each well and read at 450 nm immediately.
Background:
Protein delta homolog 1, also termed delta-like 1 (DLK1) homolog decreases, Pref-1 (preadipocyte factor 1) and fetal antigen 1 (FA1), is a protein that is encoded by the DLK1 gene.[1][2] It is a member of the EGF-like family of homeotic proteins.[3] It is expressed as a transmembrane protein, but a soluble form (sDLK1) cleaved off by ADAM17 is active in inhibiting adipogenesis,[4] that leads the differentiation of pre-adipocytes into adipocytes. Low DLK1 expression is associated with acromegaly.[4] Delta-like 1 homologue promotes tumorigenesis and epithelial-mesenchymal transition of ovarian high-grade serous carcinoma through activation of Notch signaling.[5] DKK1 is associated with extreme early-onset obesity.[6] dlk1 is a putative mammalian homeotic gene differentially expressed in small cell lung carcinoma and neuroendocrine tumor cell line.[7]
References
- Laborda J, et al. (1993). The Journal of Biological Chemistry. 268(6): 3817–20. .
- Jensen CH, et al. (1994). European Journal of Biochemistry. 225(1): 83–92.
- Laborda J (2000). Histology and Histopathology. 15(1): 119–29.
- Wang Y, Sul HS (2009). Cell Metabolism. 9(3): 287–302.
- Sze L, et al. (2019) Growth Horm. IGF Res. 45, 20-24.
- Huang CC, et al. (2019) Oncogene 38 (17), 3201-3215.
- Wermter AK, et al. (2008) Eur. J. Hum. Genet. 16 (9), 1126-1134.
- Laborda J, (1993) J. Biol. Chem. 268 (6), 3817-3820.
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