Nori Monkey IL-6Ra ELISA Kit
Price range: $508.00 through $916.00
This ELISA kit is for quantification of IL-6Ra in monkey. This is a quick ELISA assay that reduces time to 50% compared to the conventional method, and the entire assay only takes 3 hours. This assay employs the quantitative sandwich enzyme immunoassay technique and uses biotin-streptavidin chemistry to improve the performance of the assays. An antibody specific for IL-6Ra has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-6Ra present is bound by the immobilized antibody. After washing away any unbound substances, a detection antibody specific for IL-6Ra is added to the wells. Following wash to remove any unbound antibody reagent, a detection reagent is added. After intensive wash a substrate solution is added to the wells and color develops in proportion to the amount of IL-6Ra bound in the initial step. The color development is stopped, and the intensity of the color is measured.
Alternative names for IL-6Ra: Interleukin 6 receptor subunit alpha, IL6R1
This product is for laboratory research use only not for diagnostic and therapeutic purposes or any other purposes.
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Description
Nori Monkey IL-6Ra ELISA Kit Summary
Alternative names for IL-6Ra: Interleukin 6 receptor subunit alpha,
Alternative name for monkey: Non-human primate, Rhesus macaque, Cynomolgus macaque, Baboons, squirrel monkey, common marmoset, chimpanzee, capuchin monkey, spider monkey, howler monkey, golden snub-nosed monkey
| Assay Type | Solid Phase Sandwich ELISA |
| Format | 96-well Microplate or 96-Well Strip Microplate |
| Method of Detection | Colorimetric |
| Number of Targets Detected | 1 |
| Target Antigen Accession Number | A0A2K6QCX3 |
| Assay Length | 3 hours |
| Quantitative/Semiquantitative | Quantitative |
| Sample Type | Plasma, Serum, Cell Culture, Urine, Cell/Tissue Lysates, Synovial Fluid, BAL, |
| Recommended Sample Dilution (Plasma/Serum) | No dilution for sample <ULOQ; sufficient dilution for samples >ULOQ |
| Sensitivity | 3 pg/mL |
| Detection Range | 15.63-1000 pg/mL |
| Specificity | Monkey IL-6Ra |
| Cross-Reactivity | < 0.5% cross-reactivity observed with available related molecules, < 50% cross-species reactivity observed with species tested. |
| Interference | No significant interference observed with available related molecules |
| Storage/Stability | 4 ºC for up to 6 months |
| Usage | For Laboratory Research Use Only. Not for diagnostic or therapeutic use. |
| Additional Notes | The kit allows for use in multiple experiments. |
Standard Curve
Kit Components
1. Pre-coated 96-well Microplate
2. Biotinylated Detection Antibody
3. Streptavidin-HRP Conjugate
4. Lyophilized Standards
5. TMB One-Step Substrate
6. Stop Solution
7. 20 x PBS
8. Assay Buffer
Other Materials Required but not Provided:
1. Microplate Reader capable of measuring absorption at 450 nm
2. Log-log graph paper or computer and software for ELISA data analysis
3. Precision pipettes (1-1000 µl)
4. Multi-channel pipettes (300 µl)
5. Distilled or deionized water
Protocol Outline
1. Prepare all reagents, samples and standards as instructed in the datasheet.
2. Add 100 µl of Standard or samples to each well and incubate 1 h at RT.
3. Add 100 µl of Working Detection Antibody to each well and incubate 1 h at RT.
4. Add 100 µl of Working Streptavidin-HRP to each well and incubate 20 min at RT.
5. Add 100 µl of Substrate to each well and incubate 5-30 min at RT.
6. Add 50 µl of Stop Solution to each well and read at 450 nm immediately.
Background:
Interleukin 6 receptor (IL6R) also known as CD126 (Cluster of Differentiation 126) is a type I cytokine receptor. Interleukin 6 (IL6) is a potent pleiotropic cytokine that regulates cell growth and differentiation and plays an important role in immune response. Dysregulated production of IL6 and this receptor are implicated in the pathogenesis of many diseases, such as multiple myeloma, autoimmune diseases and prostate cancer. In melanocytic cells IL6R gene expression may be regulated by MITF.[1] The IL6 receptor is a protein complex consisting of a IL-6 receptor subunit (IL6R) and interleukin 6 signal transducer Glycoprotein 130. IL6R also denotes the human gene encoding this subunit. Alternatively spliced transcript variants encoding distinct isoforms have been reported.[2] IL6R subunit is also shared by many other cytokines. Interleukin-6 receptor has been shown to interact with Interleukin 6[2] [3] [4] and Ciliary neurotrophic factor.[3][5]
Reference
- Hoek KS, et al. (2008). Pigment Cell Melanoma Res. 21 (6): 665–76.
- Schwantner, Andreas et al. (2004) J. Biol. Chem. 279 (1): 571–6.
- Schuster, Björn et al. (2003) J. Biol. Chem. 278 (11): 9528–35.
- Taga, T et al. (1989) Cell 58 (3): 573–81.
- Schooltink, H (1992) FEBS Lett. 314 (3): 280–4.
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