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Nori Monkey IFNg ELISA Kit

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DataSheet  

This ELISA kit is for quantification of IFNg in monkey. This is a quick ELISA assay that reduces time to 50% compared to the conventional method, and the entire assay only takes 3 hours. This assay employs the quantitative sandwich enzyme immunoassay technique and uses biotin-streptavidin chemistry to improve the performance of the assays. An antibody specific for IFNg has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IFNg present is bound by the immobilized antibody. After washing away any unbound substances, a detection antibody specific for IFNg is added to the wells. Following wash to remove any unbound antibody reagent, a detection reagent is added. After intensive wash a substrate solution is added to the wells and color develops in proportion to the amount of IFNg bound in the initial step. The color development is stopped, and the intensity of the color is measured.

Alternative names for IFNg: Interferon gamma

This product is for Laboratory Research Use Only not for diagnostic and therapeutic purposes or any other purposes.

CAT: GR169018 Categories: , Tags: ,

Description

Nori Monkey IFNg ELISA Kit Summary

Alternative names for IFNg: Interferon gamma,

Alternative name for monkey: Rhesus macaque, cynomolgus macaque, Baboons

Assay Type Solid Phase Sandwich ELISA
Format 96-well Microplate or 96-Well Strip Microplate
Method of Detection Colorimetric
Number of Targets Detected 1
Target Antigen Accession Number P63309
Assay Length 3 hours
Quantitative/Semiquantitative Quantitative
Sample Type Plasma, Serum, Cell Culture, Urine, Cell/Tissue Lysates, Synovial Fluid, BAL,
Recommended Sample Dilution (Plasma/Serum) No dilution for sample <ULOQ; sufficient dilution for samples >ULOQ
Sensitivity  2 pg/mL
Detection Range 12.5-800 pg/mL
Specificity Monkey IFNg
Cross-Reactivity < 0.5% cross-reactivity observed with available related molecules, < 50% cross-species reactivity observed with species tested.
Interference No significant interference observed with available related molecules
Storage/Stability 4 ºC for up to 6 months
Usage For Laboratory Research Use Only. Not for diagnostic or therapeutic use.
Additional Notes The kit allows for use in multiple experiments.

 

Standard Curve

Kit Components
1. Pre-coated 96-well Microplate
2. Biotinylated Detection Antibody
3. Streptavidin-HRP Conjugate
4. Lyophilized Standards
5. TMB One-Step Substrate
6. Stop Solution
7. 20 x PBS
8. Assay Buffer

Other Materials Required but not Provided:
1. Microplate Reader capable of measuring absorption at 450 nm
2. Log-log graph paper or computer and software for ELISA data analysis
3. Precision pipettes (1-1000 µl)
4. Multi-channel pipettes (300 µl)
5. Distilled or deionized water

Protocol Outline
1. Prepare all reagents, samples and standards as instructed in the datasheet.
2. Add 100 µl of Standard or samples to each well and incubate 1 h at RT.
3. Add 100 µl of Working Detection Antibody to each well and incubate 1 h at RT.
4. Add 100 µl of Working Streptavidin-HRP to each well and incubate 20 min at RT.
5. Add 100 µl of Substrate to each well and incubate 5-30 min at RT.
6. Add 50 µl of Stop Solution to each well and read at 450 nm immediately.

Background: 

IFN-γ is a dimerized soluble cytokine that is the only member of the type II class of interferons (1). It plays key roles in both the innate and adaptive immune response against viral and intracellular bacterial infections and for tumor control (2). It alters transcription in up to 30 genes producing a variety of physiological and cellular responses. IFN-γ activates the cytotoxic activity of innate immune cells such as macrophages and NK cells (3,4). IFN-γ production by NK cells and antigen-presenting cells (APCs) promotes the cell mediated adaptive immunity by inducing IFN-γ production by T lymphocytes, increased class I and class II MHC expression, and enhancing peptide antigen presentation (3). The anti-viral activity of IFN-γ is due to its induction of PKR and other regulatory proteins. Binding of IFN-γ to the IFNGR1/IFNGR2 complex promotes dimerization of the receptor complexes to form (IFNGR1/IFNGR2)2 -IFN-γ dimer. Binding induces a conformational change in receptor intracellular domains and signaling involves Jak1, Jak2 and Stat1 (5). The critical role of IFN-γ in amplification of immune surveillance and function is supported by increased susceptibility to pathogen infection by IFN-γ or IFNGR knockout mice and in humans with inactivating mutations in IFNGR1 or IFNGR2. IFN-γ also appears to have a role in atherosclerosis (6).

References

  1. Gray PW and Goeddel DV (1982). Nature 298 (5877): 859–63.
  2. Schroder K, et al (2004). J. Leukoc. Biol. 75 (2): 163–89.
  3. Schroder, K. et al. (2004) J Leukoc Biol 75, 163-89.
  4. Martinez, F.O. et al. (2009) Annu Rev Immunol 27, 451-83.
  5. Kotenko, S.V. et al. (1995) J Biol Chem 270, 20915-21.
  6. McLaren, J.E. and Ramji, D.P. (2009) Cytokine Growth Factor Rev 20, 125-35.

Product Citations

Genorise Product Citations

Since 2014, updated on 5-Apr-26

  1. El-Ashker M et al. (2015) Vitamin C ameliorates gentamicin-induced acute kidney injury in equines: an experimental study. Equine Vet Sci. 35:1-6. Impact factor: 0.29. Products used and cited: Nori Equine IL-β, IL-6, IL-10 and IFNg ELISA kits.
  2. El-Ashker M et al. (2014) Gentamicin-induced acute kidney injury in equines is associated with marked acute phase response: an experimental study on donkey (Equus asinus). J Vet Sci Med Diagn 4:2. Impact factor: 0.65. Products used and cited: Nori Equine IL-1β, IL-6, IFN-g, IL-10, SAA, Haptoglobin, CRP and SA ELISA kits.
  3. Da Silva et al. (2014) Horse seropositive for neospora spp.: Immunoglobulin, cytokines, and c-reactive protein levels. J Equine Vet Sci. 34(10):1240-143. Impact factor: 0.882. Products used and cited: Nori Equine TNF-α, IFNg, IL-1β, IL-1RA, IL-4, IL-6 and IL-10 ELISA kits.
  4. Do Carmo GM (2015) Immunological response and markers of cell damage in seropositive horses for Toxoplasma gondii. Comparative Immunology, Microbiology and Infectious Diseases. 38:9-13. Impact factor: 1.875. Products used and cited: Nori Equine IL-1β, IL-4, IL-6, IL-10, IFNg and TNF-α ELISA Kits
  5. Post J et al. (2017) Influence of Age and Dose of African Swine Fever Virus Infections on Clinical Outcome and Blood Parameters in Pigs. Viral Immunology 30(1):58-69. Impact factor: 1.432. Products used and cited: Nori Porcine IFN-alpha/IFN-gamma ELISA Kits.
  6. Rajput IR et al. (2017) Saccharomyces boulardii and Bacillus subtilis B10 modulate TLRs and cytokines expression patterns in jejunum and ileum of broilers. PLOS ONE 12(3):e0173917. Impact factor: 2.806. Products used and cited: Nori Chicken IL-12, IL-17, IL-4, IFNg and IL-8 ELISA Kits.
  7. Pujol M et al. (2017) Variability in the response of canine and human dendritic cells stimulated with Brucella canis. Veterinary Research, 48:72. Impact factor: 2.80. Products used and cited: Nori Canine IL-17 and IFN-gamma ELISA Kits.
  8. Wu Y et al. (2018) Effects of dietary enterococcus faecium NCIMB11811 supplementation on growth performance and cellular and humoral immune responses in broiler chickens. Poultry Science http://dx.doi.org/10.3382/ps/pey368. Impact factor: 2.35. Products used and cited: Nori Chicken IL-1b, IL-2, IL-4, IL-6, IL-10, IFNg, TNFa ELISA Kits.
  9. Pi Y et al. (2019) Rubber seed oil and flaxseed oil supplementation on serum fatty acid profile, oxidative-stability of serum and milk and immune function of dairy cows. Asian-Australian J Animal Sci. 32(9):1363-1372. Impact factor: 0.971. Products used and cited: Nori Bovine IL-2, IL-4, IL-6, IL-10, IFNg, TNFa and PGE2 ELISA Kits.
  10. Lombard P et al. (2019) Clinical and immunological response in dogs naturally infected by L. infantum treated with a nutritional supplement. Animals 9(8): 501. https://doi.org/10.3390/ani9080501. Impact factor: 1.654. Products used and cited: Nori Canine IFNγ ELISA Kit.
  11. Togi et al. (2018) Effects of a p-class CpG-ODN administered by intramuscular injection on plasma cytokine and on white blood cells of healthy horses. Vet Immunol Immunopathol 201: 57-61. Impact factor: 1.846. Products used and cited: Nori Equine IFNα, IFNγ, and TNFα ELISA Kits.
  12. Sureshkumar S et al. (2019) Oral administration of lactobacillus reuteri expressing a 3D8 single-chain variable fragment (scFv) enhances chicken growth and conserves immune homeostasis. 3 Biotech 9:282. Impact factor: 1.497. Products used and cited: Nori Chicken IL-1β, IL-4, IL-6, IL-8, IFNγ, IGF1, and TNFα ELISA Kits.
  13. Sureshkumar S et al. (2020) Administration of L Salivarius expressing 3D8 scFv as a feed additive improved the growth performance, immune homeostasis, and gut microbiota of chickens. Animal Sc J DOI:10.1111/asj.13399. Impact factor: 1.714. Products and cited: Nori Chicken IL-1 beta, IL-6, IL-8, IL-4, IFN gamma, IGF1 and TNF alpha ELISA Kits (GR106294, GR106334, GR114139, GR106340, GR114082, GR106410, GR114119).
  14. Sureshkumar S. et al. (2020) Inclusion of Lactobacsllus salivarius strain revealed a positive effect on improving growth performance, fecal microbiota and immunological responses in chicken. Archives of Microbiologyorg/10.1007/s00203-020-02088-3. Impact factor: 1.607. Products used and cited: Nori Chicken IL-4, IL-6, IL-8, IL-1b, TNFa, IFNg and IGF1 ELISA Kits.
  15. Song B et al. (2021) Effects of age on immune function in broiler chickens. J Animal Sci Biotech 12:42. Impact factor: 4.167. Products used and cited: Nori Chicken IL-1β, IL-2, IL-4, IL-10 and IFNγ ELISA Kits.
  16. Nooreh Z et al. (2021) Protective and immunostimulatory effects of in-feed preparation of an anticoccidal, a probiotic, a vitamin-selenium complex, and Ferulago angulate extract in broiler chickens infected with Eimoria species. BMC Vet Res 17:307. Doi.org/10.1186/s12917-021-03005-6. Impact factor: 2.741. Products used and cited: Nori Chicken IL-6 and IFNG ELISA Kits
  17. Lee B-R et al. (2023) Increased Effect of Foot-and-Mouth Disease Virus Vaccine Struc-Tural Protein Antibody Positive Rates in Piglets Orally Treated With Zinc-Aspartic Acid. Products used and cited: Nori Porcine IFNg ELISA Kit.
  18. Sureshkumar S et al. (2022) Preliminary Study to Investigate the Effect of Lactobacillus Reuteri Administration on Growth Performance, Immunological, Gut Microbiome and Intestinal Mucosa of Chicken. Brazilian J Poult Sci 24(4):001-006. Impact factor: 0.9. http://dx.doi.org/10.1590/1806-9061-2022-1640. Products used and cited: Nori Chicken TNFa, IL-4, IL-6, IL-8, IL-1B, IFNg, IGF1 ELISA Kit.
  19. Lee B-R et al. (2023) Increased Effect of Foot-and-Mouth Disease Virus Vaccine Structural Protein Antibody Positivity Rates in Piglets Orally Treated with Amino–Zinc Complex. animals 13:2027. https://doi.org/10.3390/ani13122027. Impact factor: 3.0. Products used and cited: Nori Porcine IFNg ELISA Kit.
  20. Sureshkumar S et al. (2021) Inclusion of Lactobacillus salivariusstrain revealed a positive effect on improving growth performance, fecal microbiota and immunological responses in chicken. Archives of Microbiology 230:847-853. Impact factor: 2.8. Products used and cited: Nori Chicken IFNg, IL-8, TNFa, IL-4 ELISA Kit.
  21. Abo-Aziza FAM et al. (2022) Emergent and Neglected Equine Filariosis in Egypt: Species Diversity and Host Immune Response. pathogens 11:979. Impact factor: 3.6. https://doi.org/10.339/pathogens11090979. Products used and cited: Nori Equine IFNg, IL-5, IL-10 ELISA Kit.
  22. Adamic N et al. (2022) Effect of intrabronchial administration of autologous adipose‑derived mesenchymal stem cells on severe equine asthma. Stem Cell Res & Therapy 13:23. https://doi.org/10.1186/s13287-022-02704-7. Impact factor: 7.9. Products used and cited: Nori Equine IL-1B, IL-4, IL-8, IL-17, TNFa, IFNg ELISA Kit.
  23. Song B et al. (2022) Comparison and Correlation Analysis of Immune Function and Gut Microbiota of Broiler Chickens Raised in Double-Layer Cages and Litter Floor Pens. Microbiol Spectrum 10(4). https://doi.org/10.1128/spectrum.00045-22. Impact factor: 8. Products used and cited: Nori Chicken IL-1B, IL-4, IL-10, IFNg ELISA Kit.
  24. Song B et al. (2022) Effects of Dietary Astragalus Polysaccharide Supplementation on the Th17/Treg Balance and the Gut Microbiota of Broiler Chickens Challenged With Necrotic Enteritis. frontiers in Immunol https://doi.org/10.3389/fimmu.2022.781934. Impact factor: 6.8. Products used and cited: Nori Chicken IL-1B, IL-4, IL-10, IL-17, IFNg ELISA Kit.

 

 

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