Nori Human S100A6 ELISA Kit
$461.00 – $832.00
This ELISA kit is for quantification of S100A6 in human. This is a quick ELISA assay that reduces time to 50% compared to the conventional method, and the entire assay only takes 3 hours. This assay employs the quantitative sandwich enzyme immunoassay technique and uses biotin-streptavidin chemistry to improve the performance of the assays. An antibody specific for S100A6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any S100A6 present is bound by the immobilized antibody. After washing away any unbound substances, a detection antibody specific for S100A6 is added to the wells. Following wash to remove any unbound antibody reagent, a detection reagent is added. After intensive wash a substrate solution is added to the wells and color develops in proportion to the amount of S100A6 bound in the initial step. The color development is stopped, and the intensity of the color is measured.
Alternative names for S100A6: S100 calcium-binding protein A6, S100-A6, CACY
This product is for Laboratory Research Use Only not for diagnostic and therapeutic purposes or any other purposes.
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Description
Nori Human S100A6 ELISA Kit Summary
Alternative names for S100A6: S100 calcium-binding protein A6, S100-A6, CACY
| Assay Type | Solid Phase Sandwich ELISA |
| Format | 96-well Microplate or 96-Well Strip Microplate |
| Method of Detection | Colorimetric |
| Number of Targets Detected | 1 |
| Target Antigen Accession Number | P06703 |
| Assay Length | 3 hours |
| Quantitative/Semiquantitative | Quantitative |
| Sample Type | Plasma, Serum, Cell Culture, Urine, Cell/Tissue Lysates, Synovial Fluid, BAL, |
| Recommended Sample Dilution (Plasma/Serum) | No dilution for sample <ULOQ; sufficient dilution for samples >ULOQ |
| Sensitivity | 12 pg/mL |
| Detection Range | 62.5-4000 pg/mL |
| Specificity | Human S100A6 |
| Cross-Reactivity | < 0.5% cross-reactivity observed with available related molecules, < 50% cross-species reactivity observed with species tested. |
| Interference | No significant interference observed with available related molecules |
| Storage/Stability | 4 ºC for up to 6 months |
| Usage | For Laboratory Research Use Only. Not for diagnostic or therapeutic use. |
| Additional Notes | The kit allows for use in multiple experiments. |
Standard Curve
Kit Components
1. Pre-coated 96-well Microplate
2. Biotinylated Detection Antibody
3. Streptavidin-HRP Conjugate
4. Lyophilized Standards
5. TMB One-Step Substrate
6. Stop Solution
7. 20 x PBS
8. Assay Buffer
Other Materials Required but not Provided:
1. Microplate Reader capable of measuring absorption at 450 nm
2. Log-log graph paper or computer and software for ELISA data analysis
3. Precision pipettes (1-1000 µl)
4. Multi-channel pipettes (300 µl)
5. Distilled or deionized water
Protocol Outline
1. Prepare all reagents, samples and standards as instructed in the datasheet.
2. Add 100 µl of Standard or samples to each well and incubate 1 h at RT.
3. Add 100 µl of Working Detection Antibody to each well and incubate 1 h at RT.
4. Add 100 µl of Working Streptavidin-HRP to each well and incubate 20 min at RT.
5. Add 100 µl of Substrate to each well and incubate 5-30 min at RT.
6. Add 50 µl of Stop Solution to each well and read at 450 nm immediately.
Background:
S100 calcium-binding protein A6 (S100A6) is a protein that in humans is encoded by the S100A6 gene.[1] S100A6 is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs and is localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of several cellular processes such as cell cycle progression and differentiation. S100A6 may function in stimulation of Ca2+-dependent insulin release, stimulation of prolactin secretion, and exocytosis. Chromosomal rearrangements and altered expression of S100A6 gene have been implicated in melanoma. S100A6 has been shown to interact with S100B[2] and SUGT1.[3] S100A6 is potential diagnostic marker of papillary thyroid carcinoma.[4] aberrant S100A16 expression might be modulated by its DNA hypomethylation and serves as an independent prognostic indicator of unfavorable overall survival and recurrence-free survival in non-small cell lung adenocarcinoma.[5] S100A6 is a potential risk marker for screening of cholangiocarcinoma.[6] S100 calcium binding protein A6 (S100A6) is required for the Ca2+-dependent nuclear translocation of calcyclin binding protein (CacyBP/SIP) in colon cancer SW480 cells.[7]
References
- Murphy LC, et al. (1988) J. Biol. Chem. 263 (5), 2397-2401
- Deloulme JC, et al. (2000). J. Biol. Chem. 275 (45): 35302–10.
- Nowotny M, et al. (2003). J. Biol. Chem. 278 (29): 26923–8.
- Sofiadis A, et al. (2010). Thyroid. 20 (10): 1067–76.
- Chen, Luo L et al. (2018) PLoS ONE 13 (5), e0197402
- Onsurathum S, et al. (2018) Tumour Biol. 40 (4), 1010428318767195
- Feng S,et al. (2018) PLoS ONE 13 (3), e0192208
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