Nori Human RAB11A ELISA Kit
$461.00 – $832.00
This ELISA kit is for quantification of RAB11A in human. This is a quick ELISA assay that reduces time to 50% compared to the conventional method, and the entire assay only takes 3 hours. This assay employs the quantitative sandwich enzyme immunoassay technique and uses biotin-streptavidin chemistry to improve the performance of the assays. An antibody specific for RAB11A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any RAB11A present is bound by the immobilized antibody. After washing away any unbound substances, a detection antibody specific for RAB11A is added to the wells. Following wash to remove any unbound antibody reagent, a detection reagent is added. After intensive wash a substrate solution is added to the wells and color develops in proportion to the amount of RAB11A bound in the initial step. The color development is stopped, and the intensity of the color is measured.
Alternative names for RAB11A: Ras-related protein Rab-11A
This product is for laboratory research use only not for diagnostic and therapeutic purposes or any other purposes.
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Description
Nori Human RAB11A ELISA Kit Summary
Alternative names for RAB11A: Ras-related protein Rab-11A
| Assay Type | Solid Phase Sandwich ELISA |
| Format | 96-well Microplate or 96-Well Strip Microplate |
| Method of Detection | Colorimetric |
| Number of Targets Detected | 1 |
| Target Antigen Accession Number | P62491 |
| Assay Length | 3 hours |
| Quantitative/Semiquantitative | Quantitative |
| Sample Type | Plasma, Serum, Cell Culture, Urine, Cell/Tissue Lysates, Synovial Fluid, BAL, |
| Recommended Sample Dilution (Plasma/Serum) | No dilution for sample <ULOQ; sufficient dilution for samples >ULOQ |
| Sensitivity | 30 pg/mL |
| Detection Range | 0.156-10 ng/mL |
| Specificity | Human RAB11A |
| Cross-Reactivity | < 0.5% cross-reactivity observed with available related molecules, < 50% cross-species reactivity observed with species tested. |
| Interference | No significant interference observed with available related molecules |
| Storage/Stability | 4 ºC for up to 6 months |
| Usage | For Laboratory Research Use Only. Not for diagnostic or therapeutic use. |
| Additional Notes | The kit allows for use in multiple experiments. |
Standard Curve
Kit Components
1. Pre-coated 96-well Microplate
2. Biotinylated Detection Antibody
3. Streptavidin-HRP Conjugate
4. Lyophilized Standards
5. TMB One-Step Substrate
6. Stop Solution
7. 20 x PBS
8. Assay Buffer
Other Materials Required but not Provided:
1. Microplate Reader capable of measuring absorption at 450 nm
2. Log-log graph paper or computer and software for ELISA data analysis
3. Precision pipettes (1-1000 µl)
4. Multi-channel pipettes (300 µl)
5. Distilled or deionized water
Protocol Outline
1. Prepare all reagents, samples and standards as instructed in the datasheet.
2. Add 100 µl of Standard or samples to each well and incubate 1 h at RT.
3. Add 100 µl of Working Detection Antibody to each well and incubate 1 h at RT.
4. Add 100 µl of Working Streptavidin-HRP to each well and incubate 20 min at RT.
5. Add 100 µl of Substrate to each well and incubate 5-30 min at RT.
6. Add 50 µl of Stop Solution to each well and read at 450 nm immediately.
Background:
Ras-related protein Rab-11A is a protein that is encoded by the RAB11A gene and belongs to the small GTPase superfamily, Rab family.[1][2] It is associated with both constitutive and regulated secretory pathways, and may be involved in protein transport. Co-transport of Rab11A and IAV vRNA in infected cells and provide direct evidence that vRNA-associated Rab11A have altered transport.[3] Rab-11a controls intracellular trafficking of the innate immune receptor TLR4, and thereby also receptor signalling.[4] Flotillins mediate T cell receptor (TCR) sorting into rab5 GTP-binding protein (Rab5) and ras-related protein Rab-11A (Rab11) endosomes.[5] Rab11a was identified as a downstream target of miR-372-3p. miR-372-3p could directly bind both HULC and Rab11a.[6] Reduced levels of Rab11a expression is linked to both advanced-stage carcinoma and poorer survival rates in the colorectal cancer.[7] ab11-FIP2 functions in transferrin recycling and associates with endosomal membranes via its COOH-terminal domain.[8] A Rab11/Rip11 protein complex regulates apical membrane trafficking via recycling endosomes.[9]
References
- Drivas GT, et al. (1991) Oncogene. 6 (1): 3–9. PMID1704119.
- Gromov PS, et al. (1998). FEBS Letters. 429 (3): 359–64.
- Bhagwat AR, et al. (2020) Nat Commun 11 (1), 23.
- Husebye H, et al. (2010). Immunity. 33(4): 583–96.
- Redpath GMI, et al. (2019) Nat Commun 10 (1), 4392.
- Cao SQ, et al. (2019) World J Gastroenterol 25 (35), 5283-5299.
- D’Agostino L, et al. (2019) Cancer Res 79 (16), 4099-4112.
- Lindsay AJ, McCaffrey MW (2002). The Journal of Biological Chemistry. 277 (30): 27193–9.
- Prekeris R, et al. (2000). Molecular Cell. 6 (6): 1437–48.
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