Nori Human MUC2 ELISA Kit
$461.00 – $832.00
This ELISA kit is for quantification of MUC2 in human. This is a quick ELISA assay that reduces time to 50% compared to the conventional method, and the entire assay only takes 3 hours. This assay employs the quantitative sandwich enzyme immunoassay technique and uses biotin-streptavidin chemistry to improve the performance of the assays. An antibody specific for MUC2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MUC2 present is bound by the immobilized antibody. After washing away any unbound substances, a detection antibody specific for MUC2 is added to the wells. Following wash to remove any unbound antibody reagent, a detection reagent is added. After intensive wash a substrate solution is added to the wells and color develops in proportion to the amount of MUC2 bound in the initial step. The color development is stopped, and the intensity of the color is measured.
Alternative names for MUC2: mucin 2
This product is for Laboratory Research Use Only not for diagnostic and therapeutic purposes or any other purposes.
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Description
Nori Human MUC2 ELISA Kit Summary
Alternative names for MUC2: Mucin 2
| Assay Type | Solid Phase Sandwich ELISA |
| Format | 96-well Microplate or 96-Well Strip Microplate |
| Method of Detection | Colorimetric |
| Number of Targets Detected | 1 |
| Target Antigen Accession Number | Q02817 |
| Assay Length | 3 hours |
| Quantitative/Semiquantitative | Quantitative |
| Sample Type | Plasma, Serum, Cell Culture, Urine, Cell/Tissue Lysates, Synovial Fluid, BAL, |
| Recommended Sample Dilution (Plasma/Serum) | No dilution for sample <ULOQ; sufficient dilution for samples >ULOQ |
| Sensitivity | 150 pg/mL |
| Detection Range | 0.78-50 ng/mL |
| Specificity | Human MUC2 |
| Cross-Reactivity | < 0.5% cross-reactivity observed with available related molecules, < 50% cross-species reactivity observed with species tested. |
| Interference | No significant interference observed with available related molecules |
| Storage/Stability | 4 ºC for up to 6 months |
| Usage | For Laboratory Research Use Only. Not for diagnostic or therapeutic use. |
| Additional Notes | The kit allows for use in multiple experiments. |
Standard Curve
Kit Components
1. Pre-coated 96-well Microplate
2. Biotinylated Detection Antibody
3. Streptavidin-HRP Conjugate
4. Lyophilized Standards
5. TMB One-Step Substrate
6. Stop Solution
7. 20 x PBS
8. Assay Buffer
Other Materials Required but not Provided:
1. Microplate Reader capable of measuring absorption at 450 nm
2. Log-log graph paper or computer and software for ELISA data analysis
3. Precision pipettes (1-1000 µl)
4. Multi-channel pipettes (300 µl)
5. Distilled or deionized water
Protocol Outline
1. Prepare all reagents, samples and standards as instructed in the datasheet.
2. Add 100 µl of Standard or samples to each well and incubate 1 h at RT.
3. Add 100 µl of Working Detection Antibody to each well and incubate 1 h at RT.
4. Add 100 µl of Working Streptavidin-HRP to each well and incubate 20 min at RT.
5. Add 100 µl of Substrate to each well and incubate 5-30 min at RT.
6. Add 50 µl of Stop Solution to each well and read at 450 nm immediately.
Background:
Mucin 2 (MUC2) also known as oligomeric mucus gel-forming, is a protein that is encoded by the MUC2 gene.[1] and is a member of the mucin protein family. The protein is secreted onto mucosal surfaces.[1] Mucin 2 is particularly prominent in the gut where it is secreted from goblet cells in the epithelial lining into the lumen of the large intestine. There, mucin 2, along with small amounts of related-mucin proteins, polymerizes into a gel of which 80% by weight is oligosaccharide side-chains that are added as post-translational modifications to the mucin proteins. This gel provides an insoluble mucous barrier that serves to protect the intestinal epithelium. Endotoxins induced ECM-ieceptor interaction pathway signal effect on the function of MUC2 in caco2/HT29 co-culture cells.[2] Aberrant intestinal expression and allelic variant MUC2 is associated with inflammatory bowel disease.[3] The human MUC2 intestinal mucin has cysteine-rich subdomains located both upstream and downstream of its central repetitive region.[4]
References
- Allen A, et al. (1998). Int. J. Biochem. Cell Biol. 30 (7): 797–801.
- Hu W et al. (2022) Front Immunol 13, 916933
- Moehle C, et al. (2006) J Mol Med (Berl) 84 (12), 1055-1066
- Gum JR Jr, et al. J Biol Chem 267 (30), 21375-21383 (1992)
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