Nori Human CCL7 ELISA Kit
Price range: $508.00 through $916.00
This ELISA kit is for quantification of CCL7 in human. This is a quick ELISA assay that reduces time to 50% compared to the conventional method, and the entire assay only takes 3 hours. This assay employs the quantitative sandwich enzyme immunoassay technique and uses biotin-streptavidin chemistry to improve the performance of the assays. An antibody specific for CCL7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CCL7 present is bound by the immobilized antibody. After washing away any unbound substances, a detection antibody specific for CCL7 is added to the wells. Following wash to remove any unbound antibody reagent, a detection reagent is added. After intensive wash a substrate solution is added to the wells and color develops in proportion to the amount of CCL7 bound in the initial step. The color development is stopped, and the intensity of the color is measured.
Alternative names for CCL7: Chemokine (C-C motif) ligand 7, MCP3, monocyte-chemotactic protein 3, SCYA6, SCYA7
This product is for Laboratory Research Use Only not for diagnostic and therapeutic purposes or any other purposes.
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Description
Nori Human CCL7 ELISA Kit Summary
Alternative names for CCL7: Chemokine (C-C motif) ligand 7, monocyte-chemotactic protein 3, MCP3, SCYA6, SCYA7
| Assay Type | Solid Phase Sandwich ELISA |
| Format | 96-well Microplate or 96-Well Strip Microplate |
| Method of Detection | Colorimetric |
| Number of Targets Detected | 1 |
| Target Antigen Accession Number | P80098 |
| Assay Length | 3 hours |
| Quantitative/Semiquantitative | Quantitative |
| Sample Type | Plasma, Serum, Cell Culture, Urine, Cell/Tissue Lysates, Synovial Fluid, BAL, |
| Recommended Sample Dilution (Plasma/Serum) | No dilution for sample <ULOQ; sufficient dilution for samples >ULOQ |
| Sensitivity | 3 pg/mL |
| Detection Range | 15.6-1000 pg/mL |
| Specificity | Human CCL7 |
| Cross-Reactivity | < 0.5% cross-reactivity observed with available related molecules, < 50% cross-species reactivity observed with species tested. |
| Interference | No significant interference observed with available related molecules |
| Storage/Stability | 4 ºC for up to 6 months |
| Usage | For Laboratory Research Use Only. Not for diagnostic or therapeutic use. |
| Additional Notes | The kit allows for use in multiple experiments. |
Standard Curve
Kit Components
1. Pre-coated 96-well Microplate
2. Biotinylated Detection Antibody
3. Streptavidin-HRP Conjugate
4. Lyophilized Standards
5. TMB One-Step Substrate
6. Stop Solution
7. 20 x PBS
8. Assay Buffer
Other Materials Required but not Provided:
1. Microplate Reader capable of measuring absorption at 450 nm
2. Log-log graph paper or computer and software for ELISA data analysis
3. Precision pipettes (1-1000 µl)
4. Multi-channel pipettes (300 µl)
5. Distilled or deionized water
Protocol Outline
1. Prepare all reagents, samples and standards as instructed in the datasheet.
2. Add 100 µl of Standard or samples to each well and incubate 1 h at RT.
3. Add 100 µl of Working Detection Antibody to each well and incubate 1 h at RT.
4. Add 100 µl of Working Streptavidin-HRP to each well and incubate 20 min at RT.
5. Add 100 µl of Substrate to each well and incubate 5-30 min at RT.
6. Add 50 µl of Stop Solution to each well and read at 450 nm immediately.
Background:
Chemokine (C-C motif) ligand 7 (CCL7) is a small cytokine known as a chemokine that was previously called monocyte-chemotactic protein 3 (MCP3).[1] Due to CCL7 possessing two adjacent N-terminal cysteine residues in its mature protein, it is classified among the subfamily of chemokines known as CC chemokines. CCL7 specifically attracts monocytes, and regulates macrophage function. It is produced by certain tumor cell lines and by macrophages.[1] This chemokine is located on chromosome 17 in humans, in a large cluster containing many other CC chemokines[2] and is most closely related to CCL2 (MCP1). CCL7 has been shown to interact with MMP2.[3] MCP3 interacts with multiple leukocyte receptors such as the CCR2 (C-C chemokine receptor type 2) receptor and C-C CKR1, a receptor for macrophage inflammatory protein-1 alpha/Rantes.[4] Higher expression of CCL7 receptor CCR2 was associated with shorter overall survival of colorectal cancer patients.[5] Periprostatic adipocytes drive prostate cancer progression in obesity via CCL7 secretion which stimulates CCR3 expressing tumor cells.[6]
References
- Opdenakker G, et al. (1993) Biochem. Biophys. Res. Commun. 191 (2), 535-542.
- Opdenakker G, et al. (1994). Genomics. 21 (2): 403–8.
- McQuibban, G A; et al. (2000). Science. 289 (5482): 1202–6.
- Ben-Baruch A, et al. (1995) J. Biol. Chem. 270 (38), 22123-22128.
- Kurzejamska E, et al. (2019) Int J Mol Sci 20 (3), E686.
- Laurent V, et al. (2016) Nat Commun 7, 10230.
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