Nori Feline IL1RI ELISA Kit
$461.00 – $832.00
This ELISA kit is for quantification of IL1R1 in cat. This is a quick ELISA assay that reduces time to 50% compared to the conventional method, and the entire assay only takes 3 hours. This assay employs the quantitative sandwich enzyme immunoassay technique and uses biotin-streptavidin chemistry to improve the performance of the assays. An antibody specific for IL1R1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL1R1 present is bound by the immobilized antibody. After washing away any unbound substances, a detection antibody specific for IL1R1 is added to the wells. Following wash to remove any unbound antibody reagent, a detection reagent is added. After intensive wash a substrate solution is added to the wells and color develops in proportion to the amount of IL1R1 bound in the initial step. The color development is stopped, and the intensity of the color is measured.
Alternative names for IL1RI: Interleukin 1 receptor type I, IL-RI, IL-1RI, IL1R1,
This product is for laboratory research use only not for diagnostic and therapeutic purposes or any other purposes.
- Description
- How Elisa Works
- Product Citation (5)
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Description
Nori Feline IL1RI ELISA Kit Summary
Alternative names for IL1RI: Interleukin 1 receptor type I, IL-RI, IL-1RI, IL1R1,
Alternative names for feline: cat
| Assay Type | Solid Phase Sandwich ELISA |
| Format | 96-well Microplate or 96-Well Strip Microplate |
| Method of Detection | Colorimetric |
| Number of Targets Detected | 1 |
| Target Antigen Accession Number | A0A8I3RY59 |
| Assay Length | 3 hours |
| Quantitative/Semiquantitative | Quantitative |
| Sample Type | Plasma, Serum, Cell Culture, Urine, Cell/Tissue Lysates, Synovial Fluid, BAL, |
| Recommended Sample Dilution (Plasma/Serum) | No dilution for sample <ULOQ; sufficient dilution for samples >ULOQ |
| Sensitivity | 126 pg/mL |
| Detection Range | 62.5-4000 pg/mL |
| Specificity | Feline IL1R1 |
| Cross-Reactivity | < 0.5% cross-reactivity observed with available related molecules, < 50% cross-species reactivity observed with species tested. |
| Interference | No significant interference observed with available related molecules |
| Storage/Stability | 4 ºC for up to 6 months |
| Usage | For Laboratory Research Use Only. Not for diagnostic or therapeutic use. |
| Additional Notes | The kit allows for use in multiple experiments. |
Standard Curve
Kit Components
1. Pre-coated 96-well Microplate
2. Biotinylated Detection Antibody
3. Streptavidin-HRP Conjugate
4. Lyophilized Standards
5. TMB One-Step Substrate
6. Stop Solution
7. 20 x PBS
8. Assay Buffer
Other Materials Required but not Provided:
1. Microplate Reader capable of measuring absorption at 450 nm
2. Log-log graph paper or computer and software for ELISA data analysis
3. Precision pipettes (1-1000 µl)
4. Multi-channel pipettes (300 µl)
5. Distilled or deionized water
Protocol Outline
1. Prepare all reagents, samples and standards as instructed in the datasheet.
2. Add 100 µl of Standard or samples to each well and incubate 1 h at RT.
3. Add 100 µl of Working Detection Antibody to each well and incubate 1 h at RT.
4. Add 100 µl of Working Streptavidin-HRP to each well and incubate 20 min at RT.
5. Add 100 µl of Substrate to each well and incubate 5-30 min at RT.
6. Add 50 µl of Stop Solution to each well and read at 450 nm immediately.
Background:
Interleukin 1 receptor, type I (IL1R1) also known as CD121a (Cluster of Differentiation 121a) is encoded by IL-RI gene[1] and a member of the interleukin-1 receptor family. This gene along with interleukin 1 receptor, type II (IL1R2), interleukin 1 receptor-like 2 (IL1RL2), and interleukin 1 receptor-like 1 (IL1RL1) form a cytokine receptor gene cluster in a region mapped to chromosome 2q12.[2] This protein is a receptor for interleukin 1 alpha (IL1A), interleukin 1 beta (IL1B), and interleukin 1 receptor antagonist (IL1RA). [3] It is an important mediator involved in many cytokine induced immune and inflammatory responses. Interleukin 1 receptor, type I has been shown to interact with PIK3R1,[4] Myd88[5][6] and IL1RAP.[7] Receptor induction regulates the synergistic effects of substance P with IL-1 and platelet-derived growth factor on the proliferation of bone marrow fibroblasts.[8]
References
- Sims JE, et al. (1989). Proc. Natl. Acad. Sci. U.S.A. 86 (22): 8946–50.
- Copeland NG, et al. (1991). Genomics. 9 (1): 44–50.
- Dower SK, et al. (1987). Nature. 324 (6094): 266–8.
- Reddy, S A; Huang J H; Liao W S (1997). J. Biol. Chem. 272 (46): 29167–73.
- Burns, K; et al. (2000). Nat. Cell Biol. ENGLAND. 2 (6): 346–51.
- Muzio, M; et al. (1997). Science. 278 (5343): 1612–5.
- Huang, J; Gao X; Li S; Cao Z (1997). Natl. Acad. Sci. U.S.A. 94 (24): 12829–32.
- Rameshwar P, et al. (1997). J. Immunol. 158 (7): 3417–24.
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