Nori Equine IL-16 ELISA Kit
Price range: $508.00 through $916.00
This ELISA kit is for quantification of IL-16 in equine. This is a quick ELISA assay that reduces time to 50% compared to the conventional method, and the entire assay only takes 3 hours. This assay employs the quantitative sandwich enzyme immunoassay technique and uses biotin-streptavidin chemistry to improve the performance of the assays. An antibody specific for IL16 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL16 present is bound by the immobilized antibody. After washing away any unbound substances, a detection antibody specific for IL16 is added to the wells. Following wash to remove any unbound antibody reagent, a detection reagent is added. After intensive wash a substrate solution is added to the wells and color develops in proportion to the amount of IL16 bound in the initial step. The color development is stopped, and the intensity of the color is measured.
Alternative names for IL-16: Interleukin 16, IL16, LCF, lymphocyte, chemoattractant factor
This product is for Laboratory Research Use Only not for diagnostic and therapeutic purposes or any other purposes.
- Description
- How Elisa Works
- Documents
- Product Citations (0)
- Reviews (0)
Description
Nori Equine IL-16 ELISA Kit Summary
Alternative names for IL-16: Interleukin 16, LCF
Alternative names for equine: Horse
| Assay Type | Solid Phase Sandwich ELISA |
| Format | 96-well Microplate or 96-Well Strip Microplate |
| Method of Detection | Colorimetric |
| Number of Targets Detected | 1 |
| Target Antigen Accession Number | F6VZS8 |
| Assay Length | 3 hours |
| Quantitative/Semiquantitative | Quantitative |
| Sample Type | Plasma, Serum, Cell Culture, Urine, Cell/Tissue Lysates, Synovial Fluid, BAL, |
| Recommended Sample Dilution (Plasma/Serum) | No dilution for sample <ULOQ; sufficient dilution for samples >ULOQ |
| Sensitivity | 6 pg/mL |
| Detection Range | 31.25-2000 pg/mL |
| Specificity | Equine IL-16 |
| Cross-Reactivity | < 0.5% cross-reactivity observed with available related molecules, < 50% cross-species reactivity observed with species tested. |
| Interference | No significant interference observed with available related molecules |
| Storage/Stability | 4 ºC for up to 6 months |
| Usage | For Laboratory Research Use Only. Not for diagnostic or therapeutic use. |
| Additional Notes | The kit allows for use in multiple experiments. |
Standard Curve
Kit Components
1. Pre-coated 96-well Microplate
2. Biotinylated Detection Antibody
3. Streptavidin-HRP Conjugate
4. Lyophilized Standards
5. TMB One-Step Substrate
6. Stop Solution
7. 20 x PBS
8. Assay Buffer
Other Materials Required but not Provided:
1. Microplate Reader capable of measuring absorption at 450 nm
2. Log-log graph paper or computer and software for ELISA data analysis
3. Precision pipettes (1-1000 µl)
4. Multi-channel pipettes (300 µl)
5. Distilled or deionized water
Protocol Outline
1. Prepare all reagents, samples and standards as instructed in the datasheet.
2. Add 100 µl of Standard or samples to each well and incubate 1 h at RT.
3. Add 100 µl of Working Detection Antibody to each well and incubate 1 h at RT.
4. Add 100 µl of Working Streptavidin-HRP to each well and incubate 20 min at RT.
5. Add 100 µl of Substrate to each well and incubate 5-30 min at RT.
6. Add 50 µl of Stop Solution to each well and read at 450 nm immediately.
Background:
Interleukin-16 (IL-16) is a cytokine that is encoded by the IL16 gene.[1] The cytokine is a pleiotropic cytokine that functions as a chemoattractant, a modulator of T cell activation, and an inhibitor of HIV replication. The signaling process of this cytokine is mediated by CD4. IL-16 undergoes proteolytic processing, which is found to yield two functional proteins. The cytokine function is exclusively attributed to the secreted C-terminal peptide, while the N-terminal product may play a role in cell cycle control. Caspase 3 is reported to be involved in the proteolytic processing of IL-16. Two alternatively spliced transcript variants encoding distinct isoforms have been reported. IL-16 is released by a variety of cells (including lymphocytes and some epithelial cells) that has been characterized as a chemoattractant for certain immune cells expressing the cell surface molecule CD4. IL-16 was originally described as a factor that could attract activated T cells in human, it was previously called lymphocyte chemoattractant factor (LCF).[2] Since then, this interleukin has been shown to recruit and activate many other cells expressing the CD4 molecule, including monocytes, eosinophils, and dendritic cells.[3] The structure of IL-16 was determined following its cloning in 1994.[4] This cytokine is produced as a precursor peptide (pro-IL-16) that requires processing by an enzyme called caspase-3 to become active.[5] CD4 is the cell signaling receptor for mature IL-16.[6]
References
- Baier M, et al. (1997). Proc Natl Acad Sci U S A. 94 (10): 5273–7.
- Cruikshank W, Center DM (1982). J. Immunol. 128 (6): 2569–74.
- Cruikshank WW, et al. (2000). J. Leukoc. Biol. 67 (6): 757–66.
- Cruikshank WW, et al. (1994). Proc. Natl. Acad. Sci. U.S.A. 91 (11): 5109–13.
- Wilson KC, et al. (2005). Growth Factors. 22(2): 97–104.
- Parada NA, et al. (1996). Cell. Immunol. 168 (1): 100–6.
Product Citations
Be the first to review “Nori Equine IL-16 ELISA Kit”
You must be logged in to post a review.
Reviews
There are no reviews yet.