Nori Equine CRP ELISA Kit

$461.00$832.00

DataSheet   

This ELISA kit is for quantification of CRP in horse. This is a quick ELISA assay that reduces time to 50% compared to the conventional method, and the entire assay only takes 3 hours. This assay employs the quantitative sandwich enzyme immunoassay technique and uses biotin-streptavidin chemistry to improve the performance of the assays. An antibody specific for CRP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CRP present is bound by the immobilized antibody. After washing away any unbound substances, a detection antibody specific for CRP is added to the wells. Following wash to remove any unbound antibody reagent, a detection reagent is added. After intensive wash a substrate solution is added to the wells and color develops in proportion to the amount of CRP bound in the initial step. The color development is stopped, and the intensity of the color is measured.

Alternative names for CRP: C-reactive protein

This product is for laboratory research use only not for diagnostic and therapeutic purposes or any other purposes.

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Description

Nori Equine CRP ELISA Kit Summary

Alternative names for CRP: C-reactive protein

Alternative name for equine: Horse

Assay Type Solid Phase Sandwich ELISA
Format 96-well Microplate or 96-Well Strip Microplate
Method of Detection Colorimetric
Number of Targets Detected 1
Target Antigen Accession Number F6PZG1
Assay Length 3 hours
Quantitative/Semiquantitative Quantitative
Sample Type Plasma, Serum, Cell Culture, Urine, Cell/Tissue Lysates, Synovial Fluid, BAL,
Recommended Sample Dilution (Plasma/Serum) No dilution for sample <ULOQ; sufficient dilution for samples >ULOQ
Sensitivity 3 pg/mL
Detection Range 18.8-1200 pg/mL
Specificity Natural and recombinant equine CRP
Cross-Reactivity < 0.5% cross-reactivity observed with available related molecules, < 50% cross-species reactivity observed with species tested.
Interference No significant interference observed with available related molecules
Storage/Stability 4 ºC for up to 6 months
Usage For Laboratory Research Use Only. Not for diagnostic or therapeutic use.
Additional Notes The kit allows for use in multiple experiments.

 

Standard Curve

Kit Components
1. Pre-coated 96-well Microplate
2. Biotinylated Detection Antibody
3. Streptavidin-HRP Conjugate
4. Lyophilized Standards
5. TMB One-Step Substrate
6. Stop Solution
7. 20 x PBS
8. Assay Buffer

Other Materials Required but not Provided:
1. Microplate Reader capable of measuring absorption at 450 nm
2. Log-log graph paper or computer and software for ELISA data analysis
3. Precision pipettes (1-1000 µl)
4. Multi-channel pipettes (300 µl)
5. Distilled or deionized water

Protocol Outline
1. Prepare all reagents, samples and standards as instructed in the datasheet.
2. Add 100 µl of Standard or samples to each well and incubate 1 h at RT.
3. Add 100 µl of Working Detection Antibody to each well and incubate 1 h at RT.
4. Add 100 µl of Working Streptavidin-HRP to each well and incubate 20 min at RT.
5. Add 100 µl of Substrate to each well and incubate 5-30 min at RT.
6. Add 50 µl of Stop Solution to each well and read at 450 nm immediately.

Background:

C-reactive protein (CRP) is a protein found in the blood, the levels of which rise in response to inflammation (i.e. CRP is an acute-phase protein). CRP was first identified as a substance in the serum of patients with acute inflammation that reacted with the C-polysaccharide of Pneumococcus. Its physiological role is to bind to phosphocholine expressed on the surface of dead or dying cells (and some types of bacteria) in order to activate the complement system via the C1Q complex.[1] CRP is synthesized by the liver[2] in response to factors released by macrophages and fat cells (adipocytes).[3] It is a member of the pentraxin family of proteins.[2] C-reactive protein was the first pattern recognition receptor (PRR) to be identified.[4] CRP rises up to 50,000-fold in acute inflammation, such as infection. It rises above normal limits within 6 hours, and peaks at 48 hours. Its half-life is constant, and therefore its level is mainly determined by the rate of production (and hence the severity of the precipitating cause).

CRP is used mainly as a marker of inflammation and infection. Measuring CRP level is a screen for infectious and inflammatory diseases. Rapid, marked increases in CRP occur with inflammation, infection, trauma and tissue necrosis, malignancies, and autoimmune disorders. Because there are a large number of disparate conditions that can increase CRP production, an elevated CRP level does not diagnose a specific disease. An elevated CRP level can provide support for the presence of an inflammatory disease, such as rheumatoid arthritis, polymyalgia rheumatica or giant-cell arteritis. However, CRP level is an independent risk factor for atherosclerotic disease. Patients with high CRP concentrations are more likely to develop stroke, myocardial infarction, and severe peripheral vascular disease.[5]

 

Reference

  1. Thompson D, Pepys MB, Wood SP (1999). Structure 7 (2): 169–77
  2. Pepys MB, Hirschfield GM (June 2003). J. Clin. Invest. 111 (12): 1805–12.
  3. Lau DC, Dhillon B, Yan H, et al. (May 2005). Am. J. Physiol. Heart Circ. Physiol. 288 (5): H2031–41.
  4. Mantovani A, Garlanda C, Doni A, Bottazzi B (January 2008). J. Clin. Immunol. 28 (1): 1–13.
  5. Clearfield MB (2005). The Journal of the American Osteopathic Association 105 (9): 409–16.

 

 

 

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