Nori Canine Mcl-1 ELISA Kit
Price range: $508.00 through $916.00
This ELISA kit is for quantification of Mcl-1 in canine. This is a quick ELISA assay that reduces time to 50% compared to the conventional method, and the entire assay only takes 3 hours. This assay employs the quantitative sandwich enzyme immunoassay technique and uses biotin-streptavidin chemistry to improve the performance of the assays. An antibody specific for MCL1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MCL1 present is bound by the immobilized antibody. After washing away any unbound substances, a detection antibody specific for MCL1 is added to the wells. Following wash to remove any unbound antibody reagent, a detection reagent is added. After intensive wash a substrate solution is added to the wells and color develops in proportion to the amount of MCL1 bound in the initial step. The color development is stopped, and the intensity of the color is measured.
Alternative names for Mcl-1: Induced myeloid leukemia cell differentiation protein, MCL1
This product is for laboratory research use only not for diagnostic and therapeutic purposes or any other purposes.
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Description
Nori Canine Mcl-1 ELISA Kit Summary
Alternative names for Mcl-1: Induced myeloid leukemia cell differentiation protein, MCL1
Alternative names for canine: Dog
| Assay Type | Solid Phase Sandwich ELISA |
| Format | 96-well Microplate or 96-Well Strip Microplate |
| Method of Detection | Colorimetric |
| Number of Targets Detected | 1 |
| Target Antigen Accession Number | Q8HYS5 |
| Assay Length | 3 hours |
| Quantitative/Semiquantitative | Quantitative |
| Sample Type | Plasma, Serum, Cell Culture, Urine, Cell/Tissue Lysates, Synovial Fluid, BAL, |
| Recommended Sample Dilution (Plasma/Serum) | No dilution for sample <ULOQ; sufficient dilution for samples >ULOQ |
| Sensitivity | 25 pg/mL |
| Detection Range | 125-8000 pg/mL |
| Specificity | Canine Mcl-1 |
| Cross-Reactivity | < 0.5% cross-reactivity observed with available related molecules, < 50% cross-species reactivity observed with species tested. |
| Interference | No significant interference observed with available related molecules |
| Storage/Stability | 4 ºC for up to 6 months |
| Usage | For Laboratory Research Use Only. Not for diagnostic or therapeutic use. |
| Additional Notes | The kit allows for use in multiple experiments. |
Standard Curve
Kit Components
1. Pre-coated 96-well Microplate
2. Biotinylated Detection Antibody
3. Streptavidin-HRP Conjugate
4. Lyophilized Standards
5. TMB One-Step Substrate
6. Stop Solution
7. 20 x PBS
8. Assay Buffer
Other Materials Required but not Provided:
1. Microplate Reader capable of measuring absorption at 450 nm
2. Log-log graph paper or computer and software for ELISA data analysis
3. Precision pipettes (1-1000 µl)
4. Multi-channel pipettes (300 µl)
5. Distilled or deionized water
Protocol Outline
1. Prepare all reagents, samples and standards as instructed in the datasheet.
2. Add 100 µl of Standard or samples to each well and incubate 1 h at RT.
3. Add 100 µl of Working Detection Antibody to each well and incubate 1 h at RT.
4. Add 100 µl of Working Streptavidin-HRP to each well and incubate 20 min at RT.
5. Add 100 µl of Substrate to each well and incubate 5-30 min at RT.
6. Add 50 µl of Stop Solution to each well and read at 450 nm immediately.
Background:
Induced myeloid leukemia cell differentiation protein Mcl-1 is a protein that is encoded by the MCL1 gene.[1][2] The protein belongs to the Bcl-2 family. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified. The longer gene product (isoform 1) enhances cell survival by inhibiting apoptosis while the alternatively spliced shorter gene product (isoform 2) promotes apoptosis and is death-inducing. Omacetaxine mepesuccinate and Seliciclib [3] as an investigational treatment for chronic myelogenous leukemia (CML) and multiple myeloma respectively, act by inhibiting synthesis of Mcl-1. Mcl-1 has been shown to interact wth numerous proteins for cell signaling.
References
- Kozopas KM, et al. (1993). Proc Natl Acad Sci U S A 90 (8): 3516–20.
- Craig RW, et al. (1995). Genomics 23 (2): 457–63.
- MacCallum DE, et al. (2005). Cancer Research 65 (12): 5399–5407.
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