Nori Bovine MMP10 ELISA Kit
$461.00 – $832.00
This ELISA kit is for quantification of MMP10 in bovine. This is a quick ELISA assay that reduces time to 50% compared to the conventional method, and the entire assay only takes 3 hours. This assay employs the quantitative sandwich enzyme immunoassay technique and uses biotin-streptavidin chemistry to improve the performance of the assays. An antibody specific for MMP10 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MMP10 present is bound by the immobilized antibody. After washing away any unbound substances, a detection antibody specific for MMP10 is added to the wells. Following wash to remove any unbound antibody reagent, a detection reagent is added. After intensive wash a substrate solution is added to the wells and color develops in proportion to the amount of MMP10 bound in the initial step. The color development is stopped, and the intensity of the color is measured.
Alternative names for MMP10: Matrix metalloproteinase-10, MMP-10
This product is for Laboratory Research Use Only not for diagnostic and therapeutic purposes or any other purposes.
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Description
Nori Bovine MMP10 ELISA Kit Summary
Alternative names for MMP10: Matrix metalloproteinase-10, MMP-10
Alternative name for bovine: cow, cattle, bull
| Assay Type | Solid Phase Sandwich ELISA |
| Format | 96-well Microplate or 96-Well Strip Microplate |
| Method of Detection | Colorimetric |
| Number of Targets Detected | 1 |
| Target Antigen Accession Number | NA |
| Assay Length | 3 hours |
| Quantitative/Semiquantitative | Quantitative |
| Sample Type | Plasma, Serum, Cell Culture, Urine, Cell/Tissue Lysates, Synovial Fluid, BAL, |
| Recommended Sample Dilution (Plasma/Serum) | No dilution for sample <ULOQ; sufficient dilution for samples >ULOQ |
| Sensitivity | 5 pg/mL |
| Detection Range | 25-1600 pg/mL |
| Specificity | Bovine MMP10 |
| Cross-Reactivity | < 0.5% cross-reactivity observed with available related molecules, < 50% cross-species reactivity observed with species tested. |
| Interference | No significant interference observed with available related molecules |
| Storage/Stability | 4 ºC for up to 6 months |
| Usage | For Laboratory Research Use Only. Not for diagnostic or therapeutic use. |
| Additional Notes | The kit allows for use in multiple experiments. |
Standard Curve
Kit Components
1. Pre-coated 96-well Microplate
2. Biotinylated Detection Antibody
3. Streptavidin-HRP Conjugate
4. Lyophilized Standards
5. TMB One-Step Substrate
6. Stop Solution
7. 20 x PBS
8. Assay Buffer
Other Materials Required but not Provided:
1. Microplate Reader capable of measuring absorption at 450 nm
2. Log-log graph paper or computer and software for ELISA data analysis
3. Precision pipettes (1-1000 µl)
4. Multi-channel pipettes (300 µl)
5. Distilled or deionized water
Protocol Outline
1. Prepare all reagents, samples and standards as instructed in the datasheet.
2. Add 100 µl of Standard or samples to each well and incubate 1 h at RT.
3. Add 100 µl of Working Detection Antibody to each well and incubate 1 h at RT.
4. Add 100 µl of Working Streptavidin-HRP to each well and incubate 20 min at RT.
5. Add 100 µl of Substrate to each well and incubate 5-30 min at RT.
6. Add 50 µl of Stop Solution to each well and read at 450 nm immediately.
Background:
Matrix metalloproteinase-10 (MMP-10) also known as stromelysin-2 or transin-2 is an enzyme that is encoded by the MMP10 gene.[1] MMP10 is expressed in multiple tissues including bone.[2] MMP10 has been linked to cancer stem cell vitality and metastasis.[3] MMP-10 facilitates H. pyloripersistence and promotes gastritis.[4] MMP10 plays a role in atherosclerosis, favoring inflammation, development and complication of the plaque.[5] MMP-10 facilitates the clearance of multiwalled carbonnanotubesand moderates the pro-inflammatory response of exposed alveolar and infiltrated macrophages.[6] MMP10 serves a beneficial role in response to acute infection by moderating the proinflammatory response of resident and infiltrating macrophages.[7] MMP10 is a potential prognostic biomarker for oral cancer.[8]
References
- Madlener M, Werner S (1997). Gene. 202 (1–2): 75–81.
- Bord S, et al. (1998). Bone. 23 (1): 7–12.
- Justilien V, et al. (2012). PLOS ONE. 7 (4): e35040. doi:1371/journal.pone.0035040.
4. Lv YP et al. (2019) Sci Adv 5 (4), eaau6547.
- Purroy A, et al. (2018) Atherosclerosis 278, 124-134.
- Vandivort TC, et al. (2017) Int J Nanomedicine 12, 1019-1031.
7. McMahan RS, (2016) J. Immunol. 197 (3), 899-909
- Upadhyay P, et al. (2017). Oral Oncology. 73: 56–64.
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