Nori Bovine IL-36A ELISA Kit
$461.00 – $832.00
This ELISA kit is for quantification of IL-36A in bovine. This is a quick ELISA assay that reduces time to 50% compared to the conventional method, and the entire assay only takes 3 hours. This assay employs the quantitative sandwich enzyme immunoassay technique and uses biotin-streptavidin chemistry to improve the performance of the assays. An antibody specific for IL-36A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-36A present is bound by the immobilized antibody. After washing away any unbound substances, a detection antibody specific for IL-36A is added to the wells. Following wash to remove any unbound antibody reagent, a detection reagent is added. After intensive wash a substrate solution is added to the wells and color develops in proportion to the amount of IL-36A bound in the initial step. The color development is stopped, and the intensity of the color is measured.
Alternative names for IL-36A: IL36A, interleukin 36 alpha
This product is for laboratory research use only not for diagnostic and therapeutic purposes or any other purposes.
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Description
Nori Bovine IL-36A ELISA Kit Summary
Alternative names for IL-36A: IL36A, interleukin 36 alpha
Alternative name for bovine: Cattle, cow, bull
| Assay Type | Solid Phase Sandwich ELISA |
| Format | 96-well Microplate or 96-Well Strip Microplate |
| Method of Detection | Colorimetric |
| Number of Targets Detected | 1 |
| Target Antigen Accession Number | G3MX81 |
| Assay Length | 3 hours |
| Quantitative/Semiquantitative | Quantitative |
| Sample Type | Plasma, Serum, Cell Culture, Urine, Cell/Tissue Lysates, Synovial Fluid, BAL, |
| Recommended Sample Dilution (Plasma/Serum) | No dilution for sample <ULOQ; sufficient dilution for samples >ULOQ |
| Sensitivity | 6 pg/mL |
| Detection Range | 31.25-2000 pg/mL |
| Specificity | Bovine IL-36A |
| Cross-Reactivity | < 0.5% cross-reactivity observed with available related molecules, < 50% cross-species reactivity observed with species tested. |
| Interference | No significant interference observed with available related molecules |
| Storage/Stability | 4 ºC for up to 6 months |
| Usage | For Laboratory Research Use Only. Not for diagnostic or therapeutic use. |
| Additional Notes | The kit allows for use in multiple experiments. |
Standard Curve
Kit Components
1. Pre-coated 96-well Microplate
2. Biotinylated Detection Antibody
3. Streptavidin-HRP Conjugate
4. Lyophilized Standards
5. TMB One-Step Substrate
6. Stop Solution
7. 20 x PBS
8. Assay Buffer
Other Materials Required but not Provided:
1. Microplate Reader capable of measuring absorption at 450 nm
2. Log-log graph paper or computer and software for ELISA data analysis
3. Precision pipettes (1-1000 µl)
4. Multi-channel pipettes (300 µl)
5. Distilled or deionized water
Protocol Outline
1. Prepare all reagents, samples and standards as instructed in the datasheet.
2. Add 100 µl of Standard or samples to each well and incubate 1 h at RT.
3. Add 100 µl of Working Detection Antibody to each well and incubate 1 h at RT.
4. Add 100 µl of Working Streptavidin-HRP to each well and incubate 20 min at RT.
5. Add 100 µl of Substrate to each well and incubate 5-30 min at RT.
6. Add 50 µl of Stop Solution to each well and read at 450 nm immediately.
Background:
Interleukin-36 alpha (IL-36A) also known as interleukin-1 family member 6 (IL1F6) is a protein that in humans is encoded by the IL36A gene.[1] IL-36A is a cytokine that can activate NF-kappa-B and MAPK signaling pathways to generate an inflammatory response. The encoded protein functions primarily in skin and demonstrates increased expression in psoriasis. In addition, decreased expression of this gene has been linked to a poor prognosis in both hepatocellular carcinoma and colorectal cancer patients. IL-36-mediated IL-6 and CXCL8 production in human lung fibroblasts and bronchial epithelial cells may be involved in pulmonary inflammation especially caused by bacterial or viral infections.[2] The molecular analysis revealed strong cooperative effects of IL-17A and IL-36 cytokines in regulating target genes, which was dependent on the proteolytic activation of the latter, suggesting an amplification cycle that can be initiated by IL-17A, involving IL-36 cytokines and immune cell derived proteases and resulting in active IL-36 cytokines which synergize with IL-17A.[3] PTX3 and IL36 alpha serum levels are increased in systemic lupus erythematosus patients when compared to normal control subjects.[4] serum IL-36alpha levels were higher in active systemic lupus erythematosus patients and correlated with disease activity and arthritis.[5]
References
- Smith DE, et al. (2000). J Biol Chem. 275 (2): 1169–75.
- Zhang L and Cao J. (2017)Cytokine 99, 114-123.
- Pfaff CM, et al. (2017) Sci Rep 7 (1), 15631.
- Ismail SM, et al. (2018) Sci Rep 8 (1), 1520.
- Mai SZ, et al. (2018) Int. Immunopharmacol. 58, 103-108.
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