Nori Human GDF-15 ELISA Kit
$461.00 – $832.00
This ELISA kit is for quantification of GDF-15 in human. This is a quick ELISA assay that reduces time to 50% compared to the conventional method, and the entire assay only takes 3 hours. This assay employs the quantitative sandwich enzyme immunoassay technique and uses biotin-streptavidin chemistry to improve the performance of the assays. An antibody specific for GDF-15 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GDF-15 present is bound by the immobilized antibody. After washing away any unbound substances, a detection antibody specific for GDF-15 is added to the wells. Following wash to remove any unbound antibody reagent, a detection reagent is added. After intensive wash a substrate solution is added to the wells and color develops in proportion to the amount of GDF-15 bound in the initial step. The color development is stopped, and the intensity of the color is measured.
Alternative names for GDF-15: Growth/differentiation factor 15 (GDF15), Macrophage inhibitory cytokine-1, MIC-1, PDF, PLAB, PTGFB
This product is for laboratory research use only, not for diagnostic and therapeutic purposes or any other purposes.
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Description
Nori Human GDF-15 ELISA Kit Summary
Alternative names for GDF-15: Growth/differentiation factor 15 (GDF15), Macrophage inhibitory cytokine-1, MIC-1, PDF, PLAB, PTGFB
| Assay Type | Solid Phase Sandwich ELISA |
| Format | 96-well Microplate or 96-Well Strip Microplate |
| Method of Detection | Colorimetric |
| Number of Targets Detected | 1 |
| Target Antigen Accession Number | Q99988 |
| Assay Length | 3 hours |
| Quantitative/Semiquantitative | Quantitative |
| Sample Type | Plasma, Serum, Cell Culture, Urine, Cell/Tissue Lysates, Synovial Fluid, BAL, |
| Recommended Sample Dilution (Plasma/Serum) | No dilution for sample <ULOQ; sufficient dilution for samples >ULOQ |
| Sensitivity | 5 pg/mL |
| Detection Range | 25-1600 pg/mL |
| Specificity | Human GDF-15 |
| Cross-Reactivity | < 0.5% cross-reactivity observed with available related molecules, < 50% cross-species reactivity observed with species tested. |
| Interference | No significant interference observed with available related molecules |
| Storage/Stability | 4 ºC for up to 6 months |
| Usage | For Laboratory Research Use Only. Not for diagnostic or therapeutic use. |
| Additional Notes | The kit allows for use in multiple experiments. |
Standard Curve
Kit Components
1. Pre-coated 96-well Microplate
2. Biotinylated Detection Antibody
3. Streptavidin-HRP Conjugate
4. Lyophilized Standards
5. TMB One-Step Substrate
6. Stop Solution
7. 20 x PBS
8. Assay Buffer
Other Materials Required but not Provided:
1. Microplate Reader capable of measuring absorption at 450 nm
2. Log-log graph paper or computer and software for ELISA data analysis
3. Precision pipettes (1-1000 µl)
4. Multi-channel pipettes (300 µl)
5. Distilled or deionized water
Protocol Outline
1. Prepare all reagents, samples and standards as instructed in the datasheet.
2. Add 100 µl of Standard or samples to each well and incubate 1 h at RT.
3. Add 100 µl of Working Detection Antibody to each well and incubate 1 h at RT.
4. Add 100 µl of Working Streptavidin-HRP to each well and incubate 20 min at RT.
5. Add 100 µl of Substrate to each well and incubate 5-30 min at RT.
6. Add 50 µl of Stop Solution to each well and read at 450 nm immediately.
Background:
Growth/differentiation factor 15 (GDF15) was first identified as Macrophage inhibitory cytokine-1 or MIC-1.[1] [2] It is a protein belonging to the transforming growth factor beta superfamily. Under normal conditions, GDF-15 is expressed in low concentrations in most organs and upregulated because of injury of organs such as such as liver, kidney, heart and lung.[3][4] The function of GDF-15 is not fully cleared but is seems to have a role in regulating inflammatory pathways and to be involved in regulating apoptosis, cell repair and cell growth, which are biological processes observed in cardiovascular and neoplastic disorders.[3][5] GDF-15 protects the heart from ischemia/reperfusion injury.[6] GDF-15 has shown to be a strong prognostic protein in patients with different diseases such as heart diseases and cancer.[7]
References
- Bootcov MR, et al. (1997). Proc National Academy Sci USA. 94 (21): 11514–9.
- Ago T, Sadoshima J (2006). Circulation Research. 98 (3): 294–7.
- Zimmers TA, et al. (2005). Shock. 23 (6): 543–8.
- Hsiao EC, et al. (2000). Molecular and Cellular Biology. 20 (10): 3742–51.
- Wollert KC, et al. (2007). Circulation. 116 (14): 1540–8.
- Kempf T, et al. (2006). Circulation Research. 98 (3): 351–60.
- Wallentin L, et al. (2013). PLOS One. 8 (12): e78797.
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