Nori Human Activin A ELISA Kit
Price range: $508.00 through $916.00
This ELISA kit is for quantification of activin A in human. This is a quick ELISA assay that reduces time to 50% compared to the conventional method, and the entire assay only takes 3 hours. This assay employs the quantitative sandwich enzyme immunoassay technique and uses biotin-streptavidin chemistry to improve the performance of the assays. An antibody specific for INHBA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any INHBA present is bound by the immobilized antibody. After washing away any unbound substances, a detection antibody specific for INHBA is added to the wells. Following wash to remove any unbound antibody reagent, a detection reagent is added. After intensive wash a substrate solution is added to the wells and color develops in proportion to the amount of INHBA bound in the initial step. The color development is stopped, and the intensity of the color is measured.
Alternative names for activin A: Activin betaA Subunits, INHBA, inhibin beta A chain, erythroid differentiation protein, EDF
This product is for laboratory research use only not for diagnostic and therapeutic purposes or any other purposes.
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Description
Nori Human Activin A ELISA Kit Summary
Alternative names for activin A: Activin betaA Subunits, INHBA, inhibin beta A chain, erythroid differentiation protein, EDF
| Assay Type | Solid Phase Sandwich ELISA |
| Format | 96-well Microplate or 96-Well Strip Microplate |
| Method of Detection | Colorimetric |
| Number of Targets Detected | 1 |
| Target Antigen Accession Number | P08476 |
| Assay Length | 3 hours |
| Quantitative/Semiquantitative | Quantitative |
| Sample Type | Plasma, Serum, Cell Culture, Urine, Cell/Tissue Lysates, Synovial Fluid, BAL, |
| Recommended Sample Dilution (Plasma/Serum) | No dilution for sample <ULOQ; sufficient dilution for samples >ULOQ |
| Sensitivity | 12 pg/mL |
| Detection Range | 62.5-4000 pg/mL |
| Specificity | Human activin A |
| Cross-Reactivity | < 0.5% cross-reactivity observed with available related molecules, < 50% cross-species reactivity observed with species tested. |
| Interference | No significant interference observed with available related molecules |
| Storage/Stability | 4 ºC for up to 6 months |
| Usage | For Laboratory Research Use Only. Not for diagnostic or therapeutic use. |
| Additional Notes | The kit allows for use in multiple experiments. |
Standard Curve
Kit Components
1. Pre-coated 96-well Microplate
2. Biotinylated Detection Antibody
3. Streptavidin-HRP Conjugate
4. Lyophilized Standards
5. TMB One-Step Substrate
6. Stop Solution
7. 20 x PBS
8. Assay Buffer
Other Materials Required but not Provided:
1. Microplate Reader capable of measuring absorption at 450 nm
2. Log-log graph paper or computer and software for ELISA data analysis
3. Precision pipettes (1-1000 µl)
4. Multi-channel pipettes (300 µl)
5. Distilled or deionized water
Protocol Outline
1. Prepare all reagents, samples and standards as instructed in the datasheet.
2. Add 100 µl of Standard or samples to each well and incubate 1 h at RT.
3. Add 100 µl of Working Detection Antibody to each well and incubate 1 h at RT.
4. Add 100 µl of Working Streptavidin-HRP to each well and incubate 20 min at RT.
5. Add 100 µl of Substrate to each well and incubate 5-30 min at RT.
6. Add 50 µl of Stop Solution to each well and read at 450 nm immediately.
Background:Â
Activin A is two activin βA subunits and belongs to the TGF-β protein superfamily.[1].  Activin A is produced in the gonads, pituitary gland, placenta, and other organs. Activin A is more plentiful in the adipose tissue of obese, compared to lean persons.[2] Activin is strongly expressed in wounded skin, and overexpression of activin in epidermis of transgenic mice improves wound healing and enhances scar formation. Its action in wound repair and skin morphogenesis is through stimulation of keratinocytes and stromal cells in a dose-dependent manner.[3] Lack of activin during development results in neural developmental defects. Upregulation of Activin A drives pluripotent stem cells into a mesoendo dermal fate, and thus provides a useful tool for stem cell differentiation and organoid formation.[4] Activin A is overexpressed in many cancers. It was shown to promote tumorigenesis by hampering the adaptive anti-tumor immune response in melanoma.[27] Activin A increased the expression level of type-I collagen suggesting that activin A acts as a potent activator of fibroblasts. Activin A promotes the proliferation of adipocyte progenitor cells, while inhibiting their differentiation into adipocytes.[2] Activin A also increases inflammatory cytokines in macrophages.[2] In the ovarian follicle, activin increases FSH binding and FSH-induced aromatization. It participates in androgen synthesis enhancing LH action in the ovary and testis. In the male, activin enhances spermatogenesis. Activin also regulates the morphogenesis of branching organs such as the prostate, lung, and especially kidney. activin enhances FSH biosynthesis and secretion, and participates in the regulation of the menstrual cycle.
References
- Kingsley DM et al.(1994). Genes & Development. 8 (2): 133–46.
- Zaragosi LE, et al. (2010). Diabetes. 59 (10): 2513–2521.
- Bamberger C, et al. (2005). The American Journal of Pathology. 167 (3): 733–47.
- Pauklin S, Vallier L (2015). Development. 142 (4): 607–19.
- Donovan P, et al. (2017). The Journal of Investigative Dermatology. 137 (12): 2578–2587.
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