Nori Human TRAILR1 ELISA Kit
Price range: $508.00 through $916.00
This ELISA kit is for quantification of TRAILR1 in human. This is a quick ELISA assay that reduces time to 50% compared to the conventional method, and the entire assay only takes 3 hours. This assay employs the quantitative sandwich enzyme immunoassay technique and uses biotin-streptavidin chemistry to improve the performance of the assays. An antibody specific for TRAILR1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TRAILR1 present is bound by the immobilized antibody. After washing away any unbound substances, a detection antibody specific for TRAILR1 is added to the wells. Following wash to remove any unbound antibody reagent, a detection reagent is added. After intensive wash a substrate solution is added to the wells and color develops in proportion to the amount of TRAILR1 bound in the initial step. The color development is stopped, and the intensity of the color is measured.
Alternative names for hemopexin: TNF-related apoptosis-inducing ligand TNF-related apoptosis-inducing ligand receptor 1 (TRAILR1), Death receptor 4 (DR4), tumor necrosis factor receptor superfamily member 10A (TNFRSF10A),
This product is for laboratory research use only not for diagnostic and therapeutic purposes or any other purposes.
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Description
Nori Human TRAILR1 ELISA Kit Summary
Alternative names for TRAILR1: TNF-related apoptosis-inducing ligand TNF-related apoptosis-inducing ligand receptor 1 (TRAILR1), Death receptor 4 (DR4), tumor necrosis factor receptor superfamily member 10A (TNFRSF10A),
| Assay Type | Solid Phase Sandwich ELISA |
| Format | 96-well Microplate or 96-Well Strip Microplate |
| Method of Detection | Colorimetric |
| Number of Targets Detected | 1 |
| Target Antigen Accession Number | O00220 |
| Assay Length | 3 hours |
| Quantitative/Semiquantitative | Quantitative |
| Sample Type | Plasma, Serum, Cell Culture, Urine, Cell/Tissue Lysates, Synovial Fluid, BAL, |
| Recommended Sample Dilution (Plasma/Serum) | No dilution for sample <ULOQ; sufficient dilution for samples >ULOQ |
| Sensitivity | 25 pg/mL |
| Detection Range | 125-8000 pg/mL |
| Specificity | Human TRAILR1 |
| Cross-Reactivity | < 0.5% cross-reactivity observed with available related molecules, < 50% cross-species reactivity observed with species tested. |
| Interference | No significant interference observed with available related molecules |
| Storage/Stability | 4 ºC for up to 6 months |
| Usage | For Laboratory Research Use Only. Not for diagnostic or therapeutic use. |
| Additional Notes | The kit allows for use in multiple experiments. |
Standard Curve
Kit Components
1. Pre-coated 96-well Microplate
2. Biotinylated Detection Antibody
3. Streptavidin-HRP Conjugate
4. Lyophilized Standards
5. TMB One-Step Substrate
6. Stop Solution
7. 20 x PBS
8. Assay Buffer
Other Materials Required but not Provided:
1. Microplate Reader capable of measuring absorption at 450 nm
2. Log-log graph paper or computer and software for ELISA data analysis
3. Precision pipettes (1-1000 µl)
4. Multi-channel pipettes (300 µl)
5. Distilled or deionized water
Protocol Outline
1. Prepare all reagents, samples and standards as instructed in the datasheet.
2. Add 100 µl of Standard or samples to each well and incubate 1 h at RT.
3. Add 100 µl of Working Detection Antibody to each well and incubate 1 h at RT.
4. Add 100 µl of Working Streptavidin-HRP to each well and incubate 20 min at RT.
5. Add 100 µl of Substrate to each well and incubate 5-30 min at RT.
6. Add 50 µl of Stop Solution to each well and read at 450 nm immediately.
Background:
TNF-related apoptosis-inducing ligand TNF-related apoptosis-inducing ligand receptor 1 (TRAILR1), also known as Death receptor 4 (DR4) and tumor necrosis factor receptor superfamily member 10A (TNFRSF10A), is a cell surface receptor of the TNF-receptor superfamily that binds TRAIL and mediates apoptosis.[1] TRAILR1 is activated by tumor necrosis factor-related apoptosis inducing ligand (TNFSF10/TRAIL), and thus transduces cell death signal and induces cell apoptosis. Studies with FADD-deficient mice suggested that FADD, a death domain containing adaptor protein, is required for the apoptosis mediated by this protein.[2] DR4 induce FADD-dependent apoptosis and activate the NF-kappaB pathway.[3] TNFRSF10A has been shown to interact with DAP3.[4]
References
- Pan G, O’Rourke K, Chinnaiyan AM, Gentz R, Ebner R, Ni J, Dixit VM (April 1997). “The receptor for the cytotoxic ligand TRAIL”. Science. 276 (5309): 111–3.
- Kuang AA, Diehl GE, Zhang J, Winoto A (2000). “FADD is required for DR4- and DR5-mediated apoptosis: lack of trail-induced apoptosis in FADD-deficient mouse embryonic fibroblasts”. J. Biol. Chem. 275 (33): 25065–8.
- Chaudhary PM, Eby M, Jasmin A, et al. (1998). “Death receptor 5, a new member of the TNFR family, and DR4 induce FADD-dependent apoptosis and activate the NF-kappaB pathway”. Immunity. 7 (6): 821–30.
- Miyazaki, T; Reed J C (June 2001). “A GTP-binding adapter protein couples TRAIL receptors to apoptosis-inducing proteins”. Nat. Immunol. 2 (6): 493–500.
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