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Recombinant Sheep IL-12A Protein

Placeholder Recombinant Sheep IL-7 Protein
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DataSheet   

The recombinant sheep IL-12A protein is derived from in vitro expression of sheep IL-12A gene in E. coli and purified using his-tag affinity column and can be used in multiple applications such as cell culture, ELISA and western blot.

Alternative names for IL-12A: Interleukin 12 subunit alpha, IL12A

This product is for Laboratory Research Use Only not for diagnostic and therapeutic purposes or any other purposes.

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Description

Genorise Recombinant Sheep IL-12A Protein Summary

Alternative names for IL-12A: Interleukin 12 subunit alpha, IL12A

Alternative name for sheep: ovine, lamb, goat

Product Specifications

Purity > 96%, by SDSPAGE under reducing conditions and visualized by silver stain.
Endotoxin Level < 0.1 EU per 1 μg of the protein by the LAL method.
Activity Measured in a cell proliferation assay using PHA-stimulated human T lymphoblasts. Symons A. et al. (1987) in Lymphokines and interferons, a Practical Approach. Clemens MJ et al.(eds): IRL Press. 272.

The ED50 for this effect is typically 0.02-0.05 ng/ml.
Source E. coli derived sheep IL-12A.
Accession # Q9TU27
N-Terminal Sequence Analysis Ser
Amino Acid Sequence Gly26-Ser221
Predicted Molecular Mass 22 kDa
SDS-PAGE 22 kDa, reducing conditions

 

Background: 

IL-12 is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine and is produced by T cells, macrophages, fibroblasts, osteoblasts, endothelial and other cells (1,2,3). IL-12 induces proliferation and differentiation and acts on B cells, T cells, thymocytes, and others. IL-12 is one of the most important mediators of fever and of the acute phase response. In the muscle and fatty tissue, IL-12 stimulates energy mobilization that leads to increased body temperature. IL-12 can be secreted by macrophages in response to specific microbial molecules, referred to as pathogen associated molecular patterns (PAMPS).  IL-12 in concert with TGFβ is important for developing Th17 responses. IL-12 binds to IL-12Rα that through association induces gp130 homodimerization (1). gp130 homodimerization triggers the Jak/STAT cascade and the SHP2/Erk Map kinase cascade (1,4,5). IL-12 also forms a complex with an IL-12Rα splice variant that is non-membrane associated (4). The IL-12/soluble IL-12Rα complex can then activate the gp130 signaling pathway on cells that express gp130 but not IL6Rα (4). IL-12 is relevant to many disease processes such as diabetes (6), atherosclerosis (7), depression (8), Alzheimer’s Disease (9), systemic lupus erythematosus (10), prostate cancer (11), breast cancer (12), and rheumatoid arthritis (13).

References

  1. Heinrich, P.C. et al. (1998) Biochem J 334 ( Pt 2), 297-314.
  2. Heinrich, P.C. et al. (1998) Z Ernahrungswiss 37 Suppl 1, 43-9.
  3. Febbraio MA and Pedersen BK (2005). Exerc Sport Sci Rev 33 (3): 114–9.
  4. Jones, S.A. (2005) J Immunol 175, 3463-8.
  5. Jenkins, B.J. et al. (2004) Mol Cell Biol 24, 1453-63.
  6. Kristiansen OP and Mandrup-Poulsen T (2005). Diabetes 54 Suppl 2: S114–24.
  7. Dubiński A and Zdrojewicz Z (2007). Pol. Merkur. Lekarski 22 (130): 291–4.
  8. Dowlati Y, et al (2010). Biological Psychiatry 67 (5): 446–457.
  9. Swardfager W, et al (2010). Biological Psychiatry 68 (10): 930–941.
  10. Tackey E, et al (2004). Lupus 13 (5): 339–43.
  11. Smith PC, et al (2001). Cytokine Growth Factor Rev. 12 (1): 33–40.
  12. Hong, D.S. et al. (2007) Cancer 110, 1911-28.
  13. Nishimoto N (2006). Curr Opin Rheumatol 18 (3): 277–81

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