Recombinant Sheep IL-10 Protein
$99.00 – $456.00
DataSheet Â
The recombinant sheep IL-10 protein is derived from in vitro expression of sheep IL-10 gene in E. coli and purified using his-tag affinity column and can be used in multiple applications such as cell culture, ELISA and western blot.
Alternative names for IL-10: Interleukin 10, IL10
This product is for Laboratory Research Use Only not for diagnostic and therapeutic purposes or any other purposes.
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Description
Genorise Recombinant Sheep IL-10 Protein Summary
Alternative names for IL-10: Interleukin 10, IL10
Alternative name for sheep: ovine, lamb, goat
Product Specifications
Purity | > 96%, by SDSPAGE under reducing conditions and visualized by silver stain. |
Endotoxin Level | < 0.1 EU per 1 μg of the protein by the LAL method. |
Activity | Measured by its ability to induce myeloperoxidase release from cytochalasin B-treated human neutrophils. JM Schroder et al (1987) J Immunol 139:3474.
The ED50 for this effect is 0.2-0.4 µg/ml. |
Source | E. coli derived sheep IL-10. |
Accession # | NP_001009327.1 |
N-Terminal Sequence Analysis | Ser |
Amino Acid Sequence | Ser19-Met177 |
Predicted Molecular Mass | 18 kDa |
SDS-PAGE | 18 kDa, reducing conditions |
Background:Â
IL-10, also known as human cytokine synthesis inhibitory factor (CSIF), is an anti-inflammatory cytokine that is produced by T cells, NK cells, mast cells and macrophages (1,2,3). It is capable of inhibiting synthesis of pro-inflammatory cytokines like IFN-γ, IL-2, IL-3, TNFα and GM-CSF made by cells such as macrophages and regulatory T-cells. IL-10 also displays potent abilities to suppress the antigen presentation capacity of antigen presenting cells. IL-10 initiates signal transduction by binding to a cell surface receptor complex consisting of IL-10 RI and IL-10 RII (1). Binding of IL-10 leads to the activation of Jak1 and Tyk2, which phosphorylates Stat-3 (1,4). The anti-inflammatory activity of IL-10 is due to its ability to block signaling through other cytokine receptors, notably IFNγ receptor, by upregulating expression of SOCS-1 (1,4). In addition, IL-10 promotes T cell tolerance by inhibiting tyrosine phosphorylation of CD28 (5,6). IL-10 is an important negative regulator of the immune response, which allows for maintenance of pregnancy (1). In contrast, increased IL-10 levels contribute to persistent Leishmania major infections (7).
References
- Pestka, S. et al. (2004) Immunol Rev 202, 8-32.
- Akuffo, H. et al. (1999) Clin Exp Immunol 117, 529-34.
- Grimbaldeston MA, et al (2007). Nat. Immunol. 8 (10): 1095–104.
- O’Shea, J.J. and Murray, P.J. (2008) Immunity 28, 477-87.
- Akdis, C.A. and Blaser, K. (2001) Immunology 103, 131-6.
- Akdis, C.A. et al. (2000) FASEB J 14, 1666-8.
- Von Stebut, E. (2000) Eur J Dermatol 17, 115-22.
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