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Recombinant Porcine PDGF-AA Protein

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DataSheet   

The recombinant porcine PDGF-AA protein is derived from in vitro expression of porcine PDGFA gene in E. coli and purified using his-tag affinity column and can be used in multiple applications such as cell culture, ELISA and western blot.

Alternative names for PDGF-AA: Platelet-derived growth factor subunit A

This product is for Laboratory Research Use Only not for diagnostic and therapeutic purposes or any other purposes.

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Description

Genorise Recombinant Porcine PDGF-AA Protein Summary

Alternative names for PDGF-AA: Platelet-derived growth factor subunit A

 

Product Specifications

Purity > 96%, by SDSPAGE under reducing conditions and visualized by silver stain.
Endotoxin Level < 0.1 EU per 1 μg of the protein by the LAL method.
Activity Measured in a cell proliferation assay using NR6R-3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol 109:749. The ED50 for this effect is typically 50-200 ng/mL.
Source E. coli derived porcine PDGF-AA.
Accession # XP_020941588
N-Terminal Sequence Analysis Ser
Amino Acid Sequence Ser62-Pro196
Predicted Molecular Mass 28 kDa
SDS-PAGE 28 kDa, reducing conditions

 

Background: 

Platelet-derived growth factor subunit A is a protein that in humans is encoded by the PDGFA gene (1,2). The protein is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. This gene product can exist either as a homodimer (PDGF-AA) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB), where the dimers are connected by disulfide bonds. Mutations in this gene are associated with meningioma. Reciprocal translocations between chromosomes 22 and 17, at sites where this gene and that for COL1A1 are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans resulting from unregulated expression of growth factor. Two splice variants have been identified for this gene.

Though it is synthesized, stored and released by platelets upon activation, it is produced by a plethora of cells including smooth muscle cells, activated macrophages, and endothelial cells. PDGFs are mitogenic during early developmental stages, driving the proliferation of undifferentiated mesenchyme and some progenitor populations. During later maturation stages, PDGF signalling has been implicated in tissue remodelling and cellular differentiation, and in inductive events involved in patterning and morphogenesis. In addition to driving mesenchymal proliferation, PDGFs have been shown to direct the migration, differentiation and function of a variety of specialised mesenchymal and migratory cell types, both during development and in the adult animal (3).

Reference

  1. Ratner L, et al. (Sep 1985). Nucleic Acids Res 13 (14): 5007–18.
  2. Clements JM, et al. (Jan 1992). EMBO J 10 (13): 4113–20.
  3. Hoch RV, Soriano P (2003). Development 130 (20): 4769–4784.

 

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