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Recombinant Human TIMP1 Protein

Placeholder Recombinant Canine TIMP1 Protein
Placeholder Recombinant Canine SPINK1 Protein
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$99.00 – $2,280.00

DataSheet   

The recombinant human TIMP1 protein is derived from in vitro expression of human TIMP1 gene in E. coli and purified using his-tag affinity column and can be used in multiple applications such as cell culture, ELISA and western blot.

Alternative names for TIMP1: TIMP metallopeptidase inhibitor 1

This product is for Laboratory Research Use Only not for diagnostic and therapeutic purposes or any other purposes.

CAT: GR119220 Categories: ,
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Description

Genorise Recombinant Human TIMP1 Protein Summary

Alternative names for TIMP1: TIMP metallopeptidase inhibitor 1

 

Product Specifications

Purity > 95%, by SDSPAGE under reducing conditions and visualized by silver stain.
Endotoxin Level < 1 EU per 1 μg of the protein by the LAL method.
Activity Measured by its ability to inhibit Canine MMP­2 cleavage of a fluorogenic peptide substrate Mca-­PLGL-­Dpa­-AR-­NH2. The IC50 value is 3 nM as measured under the described conditions.
Source E. coli derived human TIMP1.
Accession # NP_003245.1
N-Terminal Sequence Analysis Met
Amino Acid Sequence Met1-Ala207
Predicted Molecular Mass 23 kDa
SDS-PAGE 23 kDa, reducing conditions

 

Background: 

TIMP metallopeptidase inhibitor 1 (TIMP1), a tissue inhibitor of metalloproteinases, a member of the TIMP family, is a glycoprotein that is expressed from the several tissues of organisms. The glycoprotein is a natural inhibitor of the matrix metalloproteinases (MMPs), a group of peptidases involved in degradation of the extracellular matrix. In addition to its inhibitory role against most of the known MMPs, the encoded protein is able to promote cell proliferation in a wide range of cell types, and may also have an anti-apoptotic function. Transcription of this gene is highly inducible in response to many cytokines and hormones. In addition, the expression from some but not all inactive X chromosomes suggests that this gene inactivation is polymorphic in Canine females. In adrenocortical cells the trophic hormone ACTH induces expression of TIMP-1 and the increase in TIMP expression is also associated with decreased collagenase activity.[1] Increased expression of TIMP1 has been found to be associated with worse prognosis of various tumors, such as laryngeal carcinoma [2] or melanoma.[3]

References

  1. Reichenstein M, et al. (2004). Cell. Endocrinol. 215 (1-2): 109–14.
  2. Ma J (2013). Int J Clin Exp Pathol. 15 (1): 246–54.
  3. Tarhini AA (2014). J Transl Med. 12.

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