Recombinant Equine TGFb3 Protein
$99.00 – $285.00
The recombinant equine TGFb3 protein is derived from in vitro expression of equine TGFb3 gene in E. coli and purified using his-tag affinity column and can be used in multiple applications such as cell culture, ELISA and western blot.
Alternative names for TGFb3: transforming growth factor beta 3
This product is for Laboratory Research Use Only not for diagnostic and therapeutic purposes or any other purposes.
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Description
Genorise Recombinant Equine TGFb3 Protein Summary
Alternative names for TGFb3: transforming growth factor beta 3
Product Specifications
Purity | > 96%, by SDSPAGE under reducing conditions and visualized by silver stain. |
Endotoxin Level | < 0.1 EU per 1 μg of the protein by the LAL method. |
Activity | Measured by its ability to inhibit the IL-4-dependent proliferation of HT-2 mouse T cells. Tsang, M et al. (1995) 7:389. The ED50 for this effect is typically 40-200 pg/mL. |
Source | E. coli derived equine TGFb3. |
Accession # | F7DR45 |
N-Terminal Sequence Analysis | Ala |
Amino Acid Sequence | Ala301 –Ser412 |
Predicted Molecular Mass | 13 kDa |
SDS-PAGE | 13 kDa, reducing conditions |
Background:
Transforming growth factor, beta 3 (TGF-b3) belongs to a large family of cytokines called the Transforming growth factor beta super family (1, 2), which includes the TGF-β family, bone morphogenetic proteins (BMPs), growth and differentiation factors (GDFs), inhibins and activins. TGF-β3 is expressed in numerous cells including epithelial cells (3). TGF-β3 is a Ser/Thr protein kinase and is believed to regulate molecules involved in cellular adhesion and extracellular matrix (ECM) formation during the process of palate development. Without TGF-β3, mammals develop a deformity known as a cleft palate (4). This is caused by failure of epithelial cells in both sides of the developing palate to fuse. TGF-b3 is involved in embryogenesis and cell differentiation. TGF-b3 signaling activates transcription of the LEF1 gene to induce epithelial mesenchymal transformation during mouse palate development (5). TGF-b3 functions in normal palate and lung morphogenesis and is implicated in epithelial-mesenchymal interaction (4). Together with other members of TGF-b, TGF-β3 functions via endoglin, an accessory protein that interacts with the receptors of the TGF-β family (6); TGF-b3 has interaction with the type I receptor ALK-1 (7).
References
- ten Dijk P. et al. (1988) Proc. Natl. Acad. Sci. 85: 4715-9.
- Derynck R. et al. (1989) EMBO J. 7:3737-43.
- Djonov V. et al. (1997) Prostate 31: 103-9.
- Kaartinen V. et al. (1995) Nature Genet. 11: 415-21.
- Nawshad A. et al. (20030 J. Cell Biol. 163: 1291-1301.
- Barbara NP et al. (1999) J. Biol. Chem. 274: 584-94.
- Lux A et al. (1999) J. Biol. Chem. 274: 9984-92.
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