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Recombinant Canine SPINK1 Protein

Placeholder Recombinant Human TIMP1 Protein
Placeholder Recombinant Human SPINK1 Protein
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DataSheet   

The recombinant canine SPINK1 protein is derived from in vitro expression of canine SPINK1 gene in E. coli and purified using his-tag affinity column and can be used in multiple applications such as cell culture, ELISA and western blot.

Alternative names for SPINK1: Pancreatic secretory trypsin inhibitor, PSTI, serine protease inhibitor Kazal-type 1, tumor-associated trypsin inhibitor, TATI

This product is for Laboratory Research Use Only not for diagnostic and therapeutic purposes or any other purposes.

CAT: GR122136 Categories: ,
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Description

Genorise Recombinant Canine SPINK1 Protein Summary

Alternative names for SPINK1: Pancreatic secretory trypsin inhibitor, PSTI, serine protease inhibitor Kazal-type 1, tumor-associated trypsin inhibitor, TATI

Alternative name for canine: Dog

Product Specifications

Purity > 95%, by SDSPAGE under reducing conditions and visualized by silver stain.
Endotoxin Level < 1 EU per 1 μg of the protein by the LAL method.
Activity Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca­RPKPVE­-Nval­-WRK (Dnp)­-NH2. The IC50 value is 12 nM, as measured under the described conditions.
Source E. coli derived canine SPINK1
Accession # XP_038513861.1
N-Terminal Sequence Analysis Met
Amino Acid Sequence Met1-Ala80
Predicted Molecular Mass 9 kDa
SDS-PAGE 9 kDa, reducing conditions

 

Background: 

Pancreatic secretory trypsin inhibitor (PSTI) also known as serine protease inhibitor Kazal-type 1 (SPINK1) or tumor-associated trypsin inhibitor (TATI) is a protein that is encoded by the SPINK1 gene.[1] Mutations in SPINK1 has been associated with hereditary pancreatitis and tropical pancreatitis. Trypsinogen is normally created and stored an inactive zymogen of trypsin in the pancreas, but occasionally will autoactivate itself. PSTI serves to cleave prematurely activated trypsin to prevent the enzyme from causing cellular damage to the organ. Without the function of PSTI, the pancreas is subject to repeated episodes of damage.[2] It has also been associated with prostate cancer.[3] SPINK1 is secreted from pancreatic acinar cells into pancreatic juice. It is thought to function in the prevention of trypsin-catalyzed premature activation of zymogens within the pancreas and the pancreatic duct. Mutations in SPINK1 gene are  associated with hereditary pancreatitis and tropical calcific pancreatitis.[4] SPINK1 contributes to proliferation and clonal formation of HT29 cells through Beclin1 associated enhanced autophagy.[5] High expression of serine protease inhibitor kazal type 1 predicts poor prognosis and promotes the progression and invasion of oral tongue squamous cell carcinoma.[6]

References

  1. Yamamoto T, et al. (1985). Biochemical and Biophysical Research Communications. 132 (2): 605–12.
  2. Marchbank T, et al. (1998). Digestion. 59 (3): 167–74.
  3. Pfützer RH, Whitcomb DC (2002). Pancreatology. 1 (5): 457–60.
  4. Witt H, et al. (2000). Nature Genetics. 25 (2): 213–6.
  5. Hu,N.A., et al. (2023) Oncol Res 30 (2), 89-97.
  6. Wang,S., et al. (2024) Arch Oral Biol 164, 106003.

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