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Recombinant Canine IL-19 Protein

Placeholder Recombinant Equine IL-1b Protein
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DataSheet   

The recombinant canine IL-19 protein is derived from in vitro expression of canine IL-19 gene in E. coli and purified using his-tag affinity column and can be used in multiple applications such as cell culture, ELISA and western blot.

Alternative names for IL-19: Interleukin 19

This product is for Laboratory Research Use Only not for diagnostic and therapeutic purposes or any other purposes.

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Description

Genorise Recombinant Canine IL-19 Protein Summary

Alternative names for IL-19: Interleukin-19

Alternative name for canine: Dog

Product Specifications

Purity > 96%, by SDSPAGE under reducing conditions and visualized by silver stain.
Endotoxin Level < 0.1 EU per 1 μg of the protein by the LAL method.
Activity Measured in a cell proliferation assay using BaF3 mouse pro-B cells transfected with human IL-20 Rαand human IL-20 Rβ. The ED50 for this effect is 0.2-0.7 ng/ml.
Source E. coli derived canine IL-19.
Accession # E2R7F8
N-Terminal Sequence Analysis Leu
Amino Acid Sequence Leu25-Ala177
Predicted Molecular Mass 18 kDa
SDS-PAGE 18 kDa, reducing conditions

 

Background: 

Interleukin-19 (IL-19) is a cytokine that is a cytokine that belongs to the IL-10 family of cytokines along with several other interleukins including IL-10, IL-20, IL-22, IL-24, IL-26, and several virus-encoded cytokines.[1] It can bind the interleukin-20 receptor complex and lead to the activation of the signal transducer and activator of transcription 3 (STAT3). A similar cytokine in mouse is reported to up-regulate the expression of IL6 and TNF-alpha and induce apoptosis, which suggests a role of this cytokine in inflammatory responses. It signals through the same cell surface receptor (IL-20R) that is used by IL-20 and IL-24. The IL-19 gene is expressed in resting monocytes and B cells. It is up-regulated in monocytes following stimulation with granulocyte-macrophage colony-stimulating factor (GM-CSF), lipopolysaccharide, or Pam3CSK4.[2][3] The expression of IL19 in biopsies of patients with active ulcerative colitis was increased compared with patients with quiescent ulcerative colitis.[4] Microbial-driven expression of IL-19 by intestinal macrophages may contribute to the pathogenesis of inflammatory bowel disease. Along with IL-20 and mda-7, IL-20 activates STAT signaling by through IL-20 receptor complexes.[5]

References

  1. Gallagher G, et al. (2000) Genes Immun. 1 (7), 442-450.
  1. Chang C, et al. (2003). The Journal of Biological Chemistry. 278 (5): 3308–13.
  2. Kragstrup TW, et al. (2015). Clinical and Experimental Immunology. 180 (2): 233–42.
  3. Steinert A, et al. (2017) J. Immunol. 199 (7), 2570-2584.
  4. Dumoutier L, et al. (2001) J. Immunol. 167 (7), 3545-3549.

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