Description

Nori Sheep IL-7 ELISA Kit Summary

Alternative names for IL-7: IL7, interleukin 7

Alternative name for sheep: ovine, lamb, goat

Standard Curve

Kit Components
1. Pre-coated 96-well Microplate
2. Biotinylated Detection Antibody
3. Streptavidin-HRP Conjugate
4. Lyophilized Standards
5. TMB One-Step Substrate
6. Stop Solution
7. 20 x PBS
8. Assay Buffer

Other Materials Required but not Provided:
1. Microplate Reader capable of measuring absorption at 450 nm
2. Log-log graph paper or computer and software for ELISA data analysis
3. Precision pipettes (1-1000 µl)
4. Multi-channel pipettes (300 µl)
5. Distilled or deionized water

Protocol Outline
1. Prepare all reagents, samples and standards as instructed in the datasheet.
2. Add 100 µl of Standard or samples to each well and incubate 1 h at RT.
3. Add 100 µl of Working Detection Antibody to each well and incubate 1 h at RT.
4. Add 100 µl of Working Streptavidin-HRP to each well and incubate 20 min at RT.
5. Add 100 µl of Substrate to each well and incubate 5-30 min at RT.
6. Add 50 µl of Stop Solution to each well and read at 450 nm immediately.

Background: 

IL-7 is a hematopoietic growth factor secreted by stromal cells in the bone marrow and thymus. It is also produced by keratinocytes,^[1] dendritic cells,^[2] hepatocytes,^[3] neurons, and epithelial cells,^[4] but is not produced by normal lymphocytes.^[5] IL-7 stimulates the differentiation of multipotent (pluripotent) hematopoietic stem cells into lymphoid progenitor cells (as opposed to myeloid progenitor cells where differentiation is stimulated by IL-3). It also stimulates proliferation of all cells in the lymphoid lineage. It is important for proliferation during certain stages of B-cell maturation, T and NK cell survival, development and homeostasis. IL-7 is a cytokine important for B and T cell development. IL-7 and the hepatocyte growth factor (HGF) form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. This cytokine is found to be a cofactor for V(D)J rearrangement of the T cell receptor beta (TCRß) during early T cell development.^[6] IL-7 can be produced locally by intestinal epithelial and epithelial goblet cells, and may serve as a regulatory factor for intestinal mucosal lymphocytes. Knockout studies in mice suggested that IL-7 plays an essential role in lymphoid cell survival. IL-7 binds to the IL-7 receptor, a heterodimer consisting of Interleukin-7 receptor alpha and common gamma chain receptor,^[7] resulting in a cascade of signals important for T-cell development within the thymus and survival within the periphery. IL-7 promotes hematological malignancies (acute lymphoblastic leukemia, T cell lymphoma).^[18]

References

1. Heufler C, et al. (1993). J. Exp. Med. 178 (3): 1109–14.
2. Kröncke R, et al. (1996). Eur. J. Immunol. 26 (10): 2541–4.
3. Sawa Y, et al. (2009). Immunity. 30 (3): 447–57.
4. Watanabe M, et al. (1995). J. Clin. Invest. 95 (6): 2945–53.
5. Fry TJ, Mackall CL (2002). Blood. 99 (11): 3892–904.
6. Muegge K, Vila MP, Durum SK (July 1993). “Interleukin-7: a cofactor for V(D)J rearrangement of the T cell receptor beta gene”. Science. 261 (5117): 93–5.
7. Noguchi M, et al. (1994). Science. 262 (5141): 1877–80.
8. Or R, et al. (1998). Cytokines Cell. Mol. Ther. 4 (4): 287–94.

Product Citation

40.        Zucca E et al. (2016) Evaluation of amniotic mesenchymal cell derivatives on cytokine production in equine
alveolar macrophages: an in vitro approach to lung inflammation. Stem Cell Research and Therapy 7:137.   Article
Product used and cited: Nori Equine TNFα/IL-6/TGFβ1 ELISA kits. Impact factor: 4.731.

91.        Witonsky S et al. (2019) Can levamisole upregulate the equine cell-mediated macrophage (M1) dendritic
cell (DC1) T-helper 1 (CD4 Th1) T-cytotoxic (CD8) immune response in vitro? J Vet Internal Med 33(2):889-96.
Impact factor: 2.286.
Products used and cited: Nori Equine IL-6 ELISA Kit.

37.        Siemieniuch MJ et al. (2016) Type of inflammation differentially affects expression of interleukin 1β and 6, tumor
necrosis factor-α and toll-like receptors in subclinical endometritis in mares. PLOS ONE 11(5):e0154934. Doi:10.137
/journal.pone.0154934. Impact factor: 2.806.
Product used and cited: Nori Equine IL-6 ELISA kit.

48. Water EVD et al. (2016) The preventive effects of two nutraceuticals on experimentally induced acute synovitis. Equine Veterinary Journal DOI:10.1111/evj.12629. Impact factor: 2.40.

Product used and cited: Nori Equine IL-6 ELISA Kit GR106001, GR106088.

81.        Abass M et al. (2018) Local mepivacaine before castration of horses under medetomidine
isoflurane balanced anesthesia is effective to reduce perioperative nociception and cytokine release.
Equine Veterinary Journal, 50:733-738. Impact factor: 2.022
Produce used and cited: Nori Equine IL-6 and TNF-alpha ELISA Kit.

96.        Broeckx SY et al. (2019) The use of equine chondrogenic-induced mesenchymal stem cells as a treatment for osteoarthritis:
A randomized, double-blinded, placebo-controlled proof-of-concept study. Equine Vet J 51(6): 787-94. Impact factor: 2.022.
Products used and cited: Nori Equine IL-6 and TGFβ3 ELISA Kits.

110.        Hussein H et al. (2016) Cathepsin K inhibition renders equine bone marrow nucleated cells hypo-responsive
to LPS and unmethylated CpG stimulation in vitro. Comparative Immunology, Microbiology and Infectious Diseases
45: 40-7. Impact factor: 2.05.
Products used and cited: Nori Equine IL-1β, IL-6 and TNFα ELISA Kits.

126. Broeckx SY et al. (2019) Evaluation of an osteochondral fragment-groove procedure for induction of
metacarpophalangeal joint osteoarthritis in horses. Am J Vet Res 80(3):246-58. Impact factor: 1.07.
         Products used and cited: Nori Equine IL-6 and TGFb3 ELISA Kits (GR106001, GR106134).