Nori Rat Angiotensinogen ELISA Kit
$461.00 – $832.00
This ELISA kit is for quantification of AGT in rat. This is a quick ELISA assay that reduces time to 50% compared to the conventional method, and the entire assay only takes 3 hours. This assay employs the quantitative sandwich enzyme immunoassay technique and uses biotin-streptavidin chemistry to improve the performance of the assays. An antibody specific for AGT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any AGT present is bound by the immobilized antibody. After washing away any unbound substances, a detection antibody specific for AGT is added to the wells. Following wash to remove any unbound antibody reagent, a detection reagent is added. After intensive wash a substrate solution is added to the wells and color develops in proportion to the amount of AGT bound in the initial step. The color development is stopped, and the intensity of the color is measured.
Alternative names for Angiotensinogen: AGT, Serpin A8, SERPINA8
This product is for laboratory research use only not for diagnostic and therapeutic purposes or any other purposes.
- Description
- How Elisa Works
- Product Citations
- Reviews (0)
Description
Nori Rat Angiotensiongen ELISA Kit Summary
Alternative names for Angiotensinogen: AGT, Serpin A8, SERPINA8
| Assay Type | Solid Phase Sandwich ELISA |
| Format | 96-well Microplate or 96-Well Strip Microplate |
| Method of Detection | Colorimetric |
| Number of Targets Detected | 1 |
| Target Antigen Accession Number |
P01015 |
| Assay Length | 3 hours |
| Quantitative/Semiquantitative | Quantitative |
| Sample Type | Plasma, Serum, Cell Culture, Urine, Cell/Tissue Lysates, Synovial Fluid, BAL, |
| Recommended Sample Dilution (Plasma/Serum) | No dilution for sample <ULOQ; sufficient dilution for samples >ULOQ |
| Sensitivity | 25 pg/mL |
| Detection Range | 125-8000 pg/mL |
| Specificity | Rat AGT |
| Cross-Reactivity | < 0.5% cross-reactivity observed with available related molecules, < 50% cross-species reactivity observed with species tested. |
| Interference | No significant interference observed with available related molecules |
| Storage/Stability | 4 ºC for up to 6 months |
| Usage | For Laboratory Research Use Only. Not for diagnostic or therapeutic use. |
| Additional Notes | The kit allows for use in multiple experiments. |
Standard Curve
Kit Components
1. Pre-coated 96-well Microplate
2. Biotinylated Detection Antibody
3. Streptavidin-HRP Conjugate
4. Lyophilized Standards
5. TMB One-Step Substrate
6. Stop Solution
7. 20 x PBS
8. Assay Buffer
Other Materials Required but not Provided:
1. Microplate Reader capable of measuring absorption at 450 nm
2. Log-log graph paper or computer and software for ELISA data analysis
3. Precision pipettes (1-1000 µl)
4. Multi-channel pipettes (300 µl)
5. Distilled or deionized water
Protocol Outline
1. Prepare all reagents, samples and standards as instructed in the datasheet.
2. Add 100 µl of Standard or samples to each well and incubate 1 h at RT.
3. Add 100 µl of Working Detection Antibody to each well and incubate 1 h at RT.
4. Add 100 µl of Working Streptavidin-HRP to each well and incubate 20 min at RT.
5. Add 100 µl of Substrate to each well and incubate 5-30 min at RT.
6. Add 50 µl of Stop Solution to each well and read at 450 nm immediately.
Background:
Angiotensinogen (AGT) also called Serpin A8 is a component of the renin-angiotensin system (RAS), a hormone system that regulates blood pressure and fluid balance.[1] It is also known as the renin substrate, and is a non-inhibitory member of the serpin family of proteinase inhibitors. Angiotensinogen is catalytically cleaved by renin to produce angiotensin I in response to lowered blood pressure. [2] Angiotensin converting enzyme (ACE), subsequently removes a dipeptide to produce angiotensin II, the physiologically active peptide, which functions in the regulation of volume and mineral balance of body. Angiotensin I and II can be further processed to generate angiotensin III, which stimulates aldosterone release and can be further processed by ACE to produce angiotensin 1-7, angiotensin 1-5 and angiotensin 1-4. Angiotensinogen is synthesized in the liver and secreted in plasma. Angiotensinogen appears to be associated with a predisposition to essential hypertension; it is also associated with pregnancy-induced hypertension (pih) (preeclampsia), a heterogeneous disorder that complicates 5-7% of all pregnancies and remains a leading cause of maternal, foetal and neonatal morbidity and mortality. Angiotensinogen plays a role in the process of Rheumatoid arthritis (RA) because angiotensin II is recognized to act as a powerful proinflammatory mediator.[3] It is also related to systemic lupus erythematosus and multiple sclerosis.
References
- Jeunemaitre X, et al. (1992)Cell 71 (1), 169-180
2. Clouston WM,et al.(1989)EMBO J. 8 (11), 3337-3343
- Chang Y et al. (2015) Clin Exp Immunol. 179(2):137-45.
Be the first to review “Nori Rat Angiotensinogen ELISA Kit”
You must be logged in to post a review.
Reviews
There are no reviews yet.