Description

Nori Rabbit IL-1b ELISA MultiSet Kit Summary

Alternative names for IL-1b: Interleukon 1 beta, IL1B

Materials provided for 10-11 of 96-well plates

Standard Curve

Protocol Outline
1. Prepare all reagents, samples and standards as instructed in the datasheet.
2.Plate preparation.

3. Add 100 µl of Standard or samples to each well and incubate 1 h at RT.
4. Add 100 µl of Working Detection Antibody to each well and incubate 1 h at RT.
5. Add 100 µl of Working Streptavidin-HRP to each well and incubate 20 min at RT.
6. Add 100 µl of Substrate to each well and incubate 5-30 min at RT.
7. Add 50 µl of Stop Solution to each well and read at 450 nm immediately.

Background: 

Interleukin-1 beta (IL-1β) also known as catabolin, is a member of the interleukin 1 cytokine family.  IL-1β precursor is cleaved by caspase 1 (interleukin 1 beta convertase). Cytosolic thiol protease cleaves the product to form mature IL-1β.  Interleukin-1 alpha and interleukin-1 beta forms IL-1 (1,2,3). IL-1β is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. IL-1β gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2 (4).

References

1. Auron PE, et al (1984). Proc Natl Acad Sci U S A 81 (24): 7907–11.
2. March CJ, et al. (1985). Nature 315 (6021): 641–7.
3. Clark BD, et al (1986). Nucleic Acids Res 14 (20): 7897–1914.
4. Entrez Gene: IL1B interleukin 1, beta. http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=3553.

Product Citations