Nori Porcine S100P ELISA Kit
$461.00 – $832.00
- DataSheet CoA SDS
This ELISA kit is for quantification of S100P in pig. This is a quick ELISA assay that reduces time to 50% compared to the conventional method, and the entire assay only takes 3 hours. This assay employs the quantitative sandwich enzyme immunoassay technique and uses biotin-streptavidin chemistry to improve the performance of the assays. An antibody specific for S100P has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any S100P present is bound by the immobilized antibody. After washing away any unbound substances, a detection antibody specific for S100P is added to the wells. Following wash to remove any unbound antibody reagent, a detection reagent is added. After intensive wash a substrate solution is added to the wells and color develops in proportion to the amount of S100P bound in the initial step. The color development is stopped, and the intensity of the color is measured.
Alternative names for S100P: S100 calcium-binding protein P, S100-P
This product is for Laboratory Research Use Only not for diagnostic and therapeutic purposes or any other purposes.
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Description
Nori Porcine S100P ELISA Kit Summary
Alternative names for S100P: S100 calcium-binding protein P, S100-P,
Alternative name for porcine: Pig
| Assay Type | Solid Phase Sandwich ELISA |
| Format | 96-well Microplate or 96-Well Strip Microplate |
| Method of Detection | Colorimetric |
| Number of Targets Detected | 1 |
| Target Antigen Accession Number | na |
| Assay Length | 3 hours |
| Quantitative/Semiquantitative | Quantitative |
| Sample Type | Plasma, Serum, Cell Culture, Urine, Cell/Tissue Lysates, Synovial Fluid, BAL, |
| Recommended Sample Dilution (Plasma/Serum) | No dilution for sample <ULOQ; sufficient dilution for samples >ULOQ |
| Sensitivity | 12 pg/mL |
| Detection Range | 62.5-4000 pg/mL |
| Specificity | Natural and recombinant porcine S100P |
| Cross-Reactivity | < 0.5% cross-reactivity observed with available related molecules, < 50% cross-species reactivity observed with species tested. |
| Interference | No significant interference observed with available related molecules |
| Storage/Stability | 4 ºC for up to 6 months |
| Usage | For Laboratory Research Use Only. Not for diagnostic or therapeutic use. |
| Additional Notes | The kit allows for use in multiple experiments. |
Standard Curve
Kit Components
1. Pre-coated 96-well Microplate
2. Biotinylated Detection Antibody
3. Streptavidin-HRP Conjugate
4. Lyophilized Standards
5. TMB One-Step Substrate
6. Stop Solution
7. 20 x PBS
8. Assay Buffer
Other Materials Required but not Provided:
1. Microplate Reader capable of measuring absorption at 450 nm
2. Log-log graph paper or computer and software for ELISA data analysis
3. Precision pipettes (1-1000 µl)
4. Multi-channel pipettes (300 µl)
5. Distilled or deionized water
Protocol Outline
1. Prepare all reagents, samples and standards as instructed in the datasheet.
2. Add 100 µl of Standard or samples to each well and incubate 1 h at RT.
3. Add 100 µl of Working Detection Antibody to each well and incubate 1 h at RT.
4. Add 100 µl of Working Streptavidin-HRP to each well and incubate 20 min at RT.
5. Add 100 µl of Substrate to each well and incubate 5-30 min at RT.
6. Add 50 µl of Stop Solution to each well and read at 450 nm immediately.
Background:
S100 calcium-binding protein P (S100P) is a protein that is encoded by the S100P gene.[1]
S100P is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100P, in addition to binding Ca2+, also binds Zn2+ and Mg2+. This protein may play a role in the etiology of prostate cancer. S100P contributes to promoter demethylation and transcriptional activation of SLC2A5 to promote metastasis in colorectal cancer.[2] Down-regulation of S100P induces apoptosis in endometrial epithelial cell during GnRH antagonist protocol.[3] S100P acts as a target of miR-495 in pancreatic cancer through bioinformatics analysis and experimental verification.[4] S100P stimulates cell proliferation and survival via receptor for activated glycation end products (RAGE).[5] Anterior gradient protein 3 and S100 calcium-binding protein P levels in different endometrial epithelial compartments may play an important role in recurrent pregnancy failure.[6] S100P Interacts with p53 while pentamidine inhibits this interaction. [7] S100P has been shown to interact with EZR[8] and RAGE.[9] The interactions between S100P and RAGE are disrupted by cromolyn[10] and pentamidine.[9]
References
- Becker T, et al. (1992). Eur. J. Biochem. 207 (2): 541–7.
- Lin M, et al. (2021) Br J Cancer 125 (5), 734-747.
- Zhang D, et al. (2021) Reprod Biol Endocrinol 19 (1), 99.
- Jiang PF, et al. (2021 Kaohsiung J Med Sci 37 (7), 562-571).
- Arumugam T, et al. (2004). J. Biol. Chem. 279 (7): 5059–65.
- Tempest N, et al. (2021) Int J Mol Sci 22 (8), 3835
- Katte RH, et al. (2021) Biomolecules 11 (5), 634.
- Koltzscher M, et al. (2003). Molecular Biology of the Cell. 14 (6): 2372–84.
- Penumutchu SR, Chou RH, Yu C (2014). PLOS ONE. 9 (8): e103947.
- Penumutchu SR, et al. (2014). Biochem Biophysical Res Communication. 454 (3): 404–409
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