Nori Porcine S100A12 ELISA Kit
$461.00 – $832.00
DataSheet CoA SDS
This ELISA kit is for quantification of S100A12 in pig. This is a quick ELISA assay that reduces time to 50% compared to the conventional method, and the entire assay only takes 3 hours. This assay employs the quantitative sandwich enzyme immunoassay technique and uses biotin-streptavidin chemistry to improve the performance of the assays. An antibody specific for S100A12 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any S100A12 present is bound by the immobilized antibody. After washing away any unbound substances, a detection antibody specific for S100A12 is added to the wells. Following wash to remove any unbound antibody reagent, a detection reagent is added. After intensive wash a substrate solution is added to the wells and color develops in proportion to the amount of S100A12 bound in the initial step. The color development is stopped, and the intensity of the color is measured.
Alternative names for S100A12: S100 calcium-binding protein A12, S100-A12, calgranulin C
This product is for Laboratory Research Use Only not for diagnostic and therapeutic purposes or any other purposes.
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Description
Nori Porcine S100A12 ELISA Kit Summary
Alternative names for S100A12: S100 calcium-binding protein A12, S100-A12, calgranulin C
Alternative name for porcine: Pig
| Assay Type | Solid Phase Sandwich ELISA |
| Format | 96-well Microplate or 96-Well Strip Microplate |
| Method of Detection | Colorimetric |
| Number of Targets Detected | 1 |
| Target Antigen Accession Number | P80310 |
| Assay Length | 3 hours |
| Quantitative/Semiquantitative | Quantitative |
| Sample Type | Plasma, Serum, Cell Culture, Urine, Cell/Tissue Lysates, Synovial Fluid, BAL, |
| Recommended Sample Dilution (Plasma/Serum) | No dilution for sample <ULOQ; sufficient dilution for samples >ULOQ |
| Sensitivity | 6 pg/mL |
| Detection Range | 31.25-2000 pg/mL |
| Specificity | Natural and recombinant porcine S100A12 |
| Cross-Reactivity | < 0.5% cross-reactivity observed with available related molecules, < 50% cross-species reactivity observed with species tested. |
| Interference | No significant interference observed with available related molecules |
| Storage/Stability | 4 ºC for up to 6 months |
| Usage | For Laboratory Research Use Only. Not for diagnostic or therapeutic use. |
| Additional Notes | The kit allows for use in multiple experiments. |
Standard Curve
Kit Components
1. Pre-coated 96-well Microplate
2. Biotinylated Detection Antibody
3. Streptavidin-HRP Conjugate
4. Lyophilized Standards
5. TMB One-Step Substrate
6. Stop Solution
7. 20 x PBS
8. Assay Buffer
Other Materials Required but not Provided:
1. Microplate Reader capable of measuring absorption at 450 nm
2. Log-log graph paper or computer and software for ELISA data analysis
3. Precision pipettes (1-1000 µl)
4. Multi-channel pipettes (300 µl)
5. Distilled or deionized water
Protocol Outline
1. Prepare all reagents, samples and standards as instructed in the datasheet.
2. Add 100 µl of Standard or samples to each well and incubate 1 h at RT.
3. Add 100 µl of Working Detection Antibody to each well and incubate 1 h at RT.
4. Add 100 µl of Working Streptavidin-HRP to each well and incubate 20 min at RT.
5. Add 100 µl of Substrate to each well and incubate 5-30 min at RT.
6. Add 50 µl of Stop Solution to each well and read at 450 nm immediately.
Background:
S100 calcium-binding protein A12 (S100A12) is a protein that in humans is encoded by the S100A12 gene.[1] It is also known as calgranulin C and belong to the S100 protein family containing 2 EF-hand calcium-binding motifs that is a novel group of proinflammatory molecules.[2] like other S100 proteins, S100A12 is localized in the cytoplasm and/or nucleus of a wide range of cells and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100A12 is a novel antimicrobial peptide [3] and is proposed to be involved in specific calcium-dependent signal transduction pathways and its regulatory effect on cytoskeletal components may modulate various neutrophil activities. CacyBP/SIP, a calcyclin and Siah-1-interacting protein, binds EF-hand proteins of the S100 family like S100A2.[4] “Neutrophil derived human S100A12 (EN-RAGE) is strongly expressed during chronic active inflammatory bowel disease.[5] S100A12 can be used as a biomarker in monitoring Kawasaki disease.[6]
Refenences
- Wicki R, et al. (1996). Cell Calcium. 20 (6): 459–64.
- Roth J, et al. (2003). Trends in Immunology. 24 (4): 155–8.
- Cole AM, et al. (2001). FEBS Letters. 504 (1–2): 5–10.
- Filipek A, et al. (2002). The Journal of Biological Chemistry. 277 (32): 28848–52.
- Foell D, et al. (2003). Gut. 52 (6): 847–53.
- Foell D, et al. (2003). Lancet. 361 (9365): 1270–2.
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