Nori Porcine IL-1RA ELISA Kit
$508.00 – $916.00
DataSheet Â
This ELISA kit is for quantification of IL-1RA in porcine. This is a quick ELISA assay that reduces time to 50% compared to the conventional method, and the entire assay only takes 3 hours. This assay employs the quantitative sandwich enzyme immunoassay technique and uses biotin-streptavidin chemistry to improve the performance of the assays. An antibody specific for IL-1RA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-1RA present is bound by the immobilized antibody. After washing away any unbound substances, a detection antibody specific for IL-1RA is added to the wells. Following wash to remove any unbound antibody reagent, a detection reagent is added. After intensive wash a substrate solution is added to the wells and color develops in proportion to the amount of IL-1RA bound in the initial step. The color development is stopped, and the intensity of the color is measured.
Alternative names for IL-1RA: Interleukin-1 receptor antagonist (IL-1RA), IL1RN, IL1F3, IL1RA, IRAP1
This product is for laboratory research use only not for diagnostic and therapeutic purposes or any other purposes.
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Description
Nori Porcine IL-1RA ELISA Kit Summary
Alternative names for IL-1RA: Interleukin-1 receptor antagonist (IL-1RA), IL1RN, IL1F3, IL1RA, IRAP1
Alternative names for porcine: pig, swine
| Assay Type | Solid Phase Sandwich ELISA |
| Format | 96-well Microplate or 96-Well Strip Microplate |
| Method of Detection | Colorimetric |
| Number of Targets Detected | 1 |
| Target Antigen Accession Number | Q29056 |
| Assay Length | 3 hours |
| Quantitative/Semiquantitative | Quantitative |
| Sample Type | Plasma, Serum, Cell Culture, Urine, Cell/Tissue Lysates, Synovial Fluid, BAL, |
| Recommended Sample Dilution (Plasma/Serum) | No dilution for sample <ULOQ; sufficient dilution for samples >ULOQ |
| Sensitivity | 5 pg/mL |
| Detection Range | 28-1800 pg/mL |
| Specificity | Porcine IL-1RA |
| Cross-Reactivity | < 0.5% cross-reactivity observed with available related molecules, < 50% cross-species reactivity observed with species tested. |
| Interference | No significant interference observed with available related molecules |
| Storage/Stability | 4 ºC for up to 6 months |
| Usage | For Laboratory Research Use Only. Not for diagnostic or therapeutic use. |
| Additional Notes | The kit allows for use in multiple experiments. |
Standard Curve
Kit Components
1. Pre-coated 96-well Microplate
2. Biotinylated Detection Antibody
3. Streptavidin-HRP Conjugate
4. Lyophilized Standards
5. TMB One-Step Substrate
6. Stop Solution
7. 20 x PBS
8. Assay Buffer
Other Materials Required but not Provided:
1. Microplate Reader capable of measuring absorption at 450 nm
2. Log-log graph paper or computer and software for ELISA data analysis
3. Precision pipettes (1-1000 µl)
4. Multi-channel pipettes (300 µl)
5. Distilled or deionized water
Protocol Outline
1. Prepare all reagents, samples and standards as instructed in the datasheet.
2. Add 100 µl of Standard or samples to each well and incubate 1 h at RT.
3. Add 100 µl of Working Detection Antibody to each well and incubate 1 h at RT.
4. Add 100 µl of Working Streptavidin-HRP to each well and incubate 20 min at RT.
5. Add 100 µl of Substrate to each well and incubate 5-30 min at RT.
6. Add 50 µl of Stop Solution to each well and read at 450 nm immediately.
Background:Â
The interleukin-1 receptor antagonist (IL-1RA) is a member of the interleukin 1 cytokine family. IL1ra is secreted by various types of cells including immune cells, epithelial cells, and adipocytes, and is a natural inhibitor of the pro-inflammatory effect of IL1β (1). This protein binds non-productively to the cell surface interleukin-1 receptor (IL-1R), and inhibits the activities of interleukin 1, alpha (IL1A) and interleukin 1, beta (IL1β), and modulates a variety of interleukin 1 related immune and inflammatory responses. This gene and five other closely related cytokine genes form a gene cluster spanning approximately 400 kb on chromosome 2. Four alternatively spliced transcript variants encoding distinct isoforms have been reported (2). A polymorphism of this gene is reported to be associated with increased risk of osteoporotic fractures and gastric cancer. Mutations in the IL1ra gene results in a rare disease called deficiency of the interleukin-1–receptor antagonist (DIRA). Variants of the IL1ra gene is also associated with risk of schizophrenia. Elevated levels of IL1ra has been found in serum of schizophrenia patients. An interleukin 1 receptor antagonist is used in the treatment of rheumatoid arthritis, an autoimmune disease in which IL-1 plays a key role.
References
- Perrier S, et al. (2006). FEBS Lett. 580(27):6289.
- Entrez Gene: IL1RN interleukin 1 receptor antagonist.
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