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photo-ELISA Kit
photo-ELISA Kit

Nori Human TLR1 ELISA Kit

Placeholder Recombinant Human SPINK1 Protein
Placeholder Recombinant Human MMP-1 Protein
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$508.00 – $916.00

DataSheet   

This ELISA kit is for quantification of TLR1 in human. This is a quick ELISA assay that reduces time to 50% compared to the conventional method, and the entire assay only takes 3 hours. This assay employs the quantitative sandwich enzyme immunoassay technique and uses biotin-streptavidin chemistry to improve the performance of the assays. An antibody specific for TLR1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TLR1 present is bound by the immobilized antibody. After washing away any unbound substances, a detection antibody specific for TLR1 is added to the wells. Following wash to remove any unbound antibody reagent, a detection reagent is added. After intensive wash a substrate solution is added to the wells and color develops in proportion to the amount of TLR1 bound in the initial step. The color development is stopped, and the intensity of the color is measured.

Alternative names for TLR1: Toll-like receptor 1 (TLR1), CD281

This product is for laboratory research use only not for diagnostic and therapeutic purposes or any other purposes.

CAT: GR106054 Categories: Human, Toll-like Receptor ELISA Kits Tag: TLR
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Description

Nori Human TLR1 ELISA Kit Summary

Alternative names for TLR1: Toll-like receptor 1 (TLR1), CD281

 

Assay Type Solid Phase Sandwich ELISA
Format 96-well Microplate or 96-Well Strip Microplate
Method of Detection Colorimetric
Number of Targets Detected 1
Target Antigen Accession Number
Q15399
Assay Length 3 hours
Quantitative/Semiquantitative Quantitative
Sample Type Plasma, Serum, Cell Culture, Urine, Cell/Tissue Lysates, Synovial Fluid, BAL,
Recommended Sample Dilution (Plasma/Serum) No dilution for sample <ULOQ; sufficient dilution for samples >ULOQ
Sensitivity 25 pg/mL
Detection Range 125-8000 pg/mL
Specificity Human TLR1
Cross-Reactivity < 0.5% cross-reactivity observed with available related molecules, < 50% cross-species reactivity observed with species tested.
Interference No significant interference observed with available related molecules
Storage/Stability 4 ºC for up to 6 months
Usage For Laboratory Research Use Only. Not for diagnostic or therapeutic use.
Additional Notes The kit allows for use in multiple experiments.

 

Standard Curve

 

Kit Components
1. Pre-coated 96-well Microplate
2. Biotinylated Detection Antibody
3. Streptavidin-HRP Conjugate
4. Lyophilized Standards
5. TMB One-Step Substrate
6. Stop Solution
7. 20 x PBS
8. Assay Buffer

Other Materials Required but not Provided:
1. Microplate Reader capable of measuring absorption at 450 nm
2. Log-log graph paper or computer and software for ELISA data analysis
3. Precision pipettes (1-1000 µl)
4. Multi-channel pipettes (300 µl)
5. Distilled or deionized water

Protocol Outline
1. Prepare all reagents, samples and standards as instructed in the datasheet.
2. Add 100 µl of Standard or samples to each well and incubate 1 h at RT.
3. Add 100 µl of Working Detection Antibody to each well and incubate 1 h at RT.
4. Add 100 µl of Working Streptavidin-HRP to each well and incubate 20 min at RT.
5. Add 100 µl of Substrate to each well and incubate 5-30 min at RT.
6. Add 50 µl of Stop Solution to each well and read at 450 nm immediately.

Background:

TLR1 is a member of the Toll-like receptor family (TLR) of pattern recognition receptors of the innate immune system (1,2). TLR1 recognizes pathogen-associated molecular pattern with a specificity for gram-positive bacteria. TLR1 has also been designated as CD281 (cluster of differentiation 281).

TLRs TLRs contain a large number of leucinerich repeats (LRRs) and a cytoplasmic tail with one Toll/IL1 receptor (TIR) domain.They are type I transmembrane proteins that serve as pattern recognition receptors for microbial pathogens,  and highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is ubiquitously expressed, and at higher levels than other TLR genes. Different length transcripts presumably resulting from use of alternative polyadenylation site, and/or from alternative splicing, have been noted for this gene (3).

TLR1 recognises peptidoglycan and (triacyl) lipoproteins in concert with TLR2 (as a heterodimer) (4, 5). It is found on the surface of macrophages and neutrophils. TLR 1 has been shown to interact with TLR 2 (6).

References

  1. Rock FL, et al. Proc. Natl. Acad. Sci. U.S.A. 95 (2): 588–93.
  2. Lien E, et al. Crit. Care Med. 30 (1 Suppl): S1–11.
  3. “SRF serum response factor”. Entrez Gene. National Center for Biotechnology Information, National Institutes of Health.
  4. Farhat K, et al. J. Leukoc. Biol. 83 (3): 692–701.
  5. Jin MS, et al. Cell 130 (6): 1071–82.
  6. Osamu T, et al. J. Immunol. (United States) 169 (1): 10–4.

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