Nori Human SVA2 ELISA Kit
$559.00 – $1,008.00
This ELISA kit is for quantification of SV2A in human. This is a quick ELISA assay that reduces time to 50% compared to the conventional method, and the entire assay only takes 3 hours. This assay employs the quantitative sandwich enzyme immunoassay technique and uses biotin-streptavidin chemistry to improve the performance of the assays. An antibody specific for SV2A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SV2A present is bound by the immobilized antibody. After washing away any unbound substances, a detection antibody specific for SV2A is added to the wells. Following wash to remove any unbound antibody reagent, a detection reagent is added. After intensive wash a substrate solution is added to the wells and color develops in proportion to the amount of SV2A bound in the initial step. The color development is stopped, and the intensity of the color is measured.
Alternative names for SV2A: Synaptic vesicle glycoprotein 2A
This product is for laboratory research use only not for diagnostic and therapeutic purposes or any other purposes.
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Description
Nori Human SVA2 ELISA Kit Summary
Alternative names for SVA2: Synaptic vesicle glycoprotein 2A
| Assay Type | Solid Phase Sandwich ELISA |
| Format | 96-well Microplate or 96-Well Strip Microplate |
| Method of Detection | Colorimetric |
| Number of Targets Detected | 1 |
| Target Antigen Accession Number | Q7L0J3 |
| Assay Length | 3 hours |
| Quantitative/Semiquantitative | Quantitative |
| Sample Type | Plasma, Serum, Cell Culture, Urine, Cell/Tissue Lysates, Synovial Fluid, BAL, |
| Recommended Sample Dilution (Plasma/Serum) | No dilution for sample <ULOQ; sufficient dilution for samples >ULOQ |
| Sensitivity | 300 pg/mL |
| Detection Range | 1.563-100 ng/mL |
| Specificity | Human SV2A |
| Cross-Reactivity | < 0.5% cross-reactivity observed with available related molecules, < 50% cross-species reactivity observed with species tested. |
| Interference | No significant interference observed with available related molecules |
| Storage/Stability | 4 ºC for up to 6 months |
| Usage | For Laboratory Research Use Only. Not for diagnostic or therapeutic use. |
| Additional Notes | The kit allows for use in multiple experiments. |
Standard Curve
Kit Components
1. Pre-coated 96-well Microplate
2. Biotinylated Detection Antibody
3. Streptavidin-HRP Conjugate
4. Lyophilized Standards
5. TMB One-Step Substrate
6. Stop Solution
7. 20 x PBS
8. Assay Buffer
Other Materials Required but not Provided:
1. Microplate Reader capable of measuring absorption at 450 nm
2. Log-log graph paper or computer and software for ELISA data analysis
3. Precision pipettes (1-1000 µl)
4. Multi-channel pipettes (300 µl)
5. Distilled or deionized water
Protocol Outline
1. Prepare all reagents, samples and standards as instructed in the datasheet.
2. Add 100 µl of Standard or samples to each well and incubate 1 h at RT.
3. Add 100 µl of Working Detection Antibody to each well and incubate 1 h at RT.
4. Add 100 µl of Working Streptavidin-HRP to each well and incubate 20 min at RT.
5. Add 100 µl of Substrate to each well and incubate 5-30 min at RT.
6. Add 50 µl of Stop Solution to each well and read at 450 nm immediately.
Background:
Synaptic vesicle glycoprotein 2A (SV2A) is a ubiquitous synaptic vesicle protein that is encoded by the SV2A gene.[1][2] It is present in every synapse of the human nervous system, irrespective of neurotransmitter type.[3] The synaptic vesicle protein SV2 is complexed with an alpha5-containing laminin on the nerve terminal surface.[4] SV2A is one of three related synaptic vesicle proteins and may interact with synaptotagmin to enhance low frequency neurotransmission in quiescent neurons. Biallelic variants in SV2A are associated with epileptic encephalopathy.[5] The synaptic vesicle protein SV2A is the binding site for the antiepileptic drug levetiracetam.[6]
References
- Bajjalieh SM, et al. (1993). PNAS USA. 90 (6): 2150–4.
- Crowder KM, et al. (1999). PNAS USA. 96 (26): 15268–73.
- Rossi, Rachele; et al. (2022). Frontiers in Neuroscience. 16: 864514.
- Son YJ, et al. (2000). The Journal of Biological Chemistry. 275 (1): 451–60.
- Al-Maawali,A., et al. (2024) Eur J Hum Genet 32 (2), 243-246.
- Lynch,B.A., et al. (2004) Proc Natl Acad Sci U S A 101 (26), 9861-9866
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