Nori Human IL-9 ELISA Kit
$461.00 – $832.00
This ELISA kit is for quantification of IL-9 in human. This is a quick ELISA assay that reduces time to 50% compared to the conventional method, and the entire assay only takes 3 hours. This assay employs the quantitative sandwich enzyme immunoassay technique and uses biotin-streptavidin chemistry to improve the performance of the assays. An antibody specific for IL-9 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-9 present is bound by the immobilized antibody. After washing away any unbound substances, a detection antibody specific for IL-9 is added to the wells. Following wash to remove any unbound antibody reagent, a detection reagent is added. After intensive wash a substrate solution is added to the wells and color develops in proportion to the amount of IL-9 bound in the initial step. The color development is stopped, and the intensity of the color is measured.
Alternative names for IL-9: IL9, interleukin 9,
This product is for laboratory research use only not for diagnostic and therapeutic purposes or any other purposes.
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Description
Nori Human IL-9 ELISA Kit Summary
Alternative names for IL-9: IL9, interleukin 9,
| Assay Type | Solid Phase Sandwich ELISA |
| Format | 96-well Microplate or 96-Well Strip Microplate |
| Method of Detection | Colorimetric |
| Number of Targets Detected | 1 |
| Target Antigen Accession Number | P15248 |
| Assay Length | 3 hours |
| Quantitative/Semiquantitative | Quantitative |
| Sample Type | Plasma, Serum, Cell Culture, Urine, Cell/Tissue Lysates, Synovial Fluid, BAL, |
| Recommended Sample Dilution (Plasma/Serum) | No dilution for sample <ULOQ; sufficient dilution for samples >ULOQ |
| Sensitivity | 3 pg/mL |
| Detection Range | 15.625-1000 pg/mL |
| Specificity | Human IL-9 |
| Cross-Reactivity | < 0.5% cross-reactivity observed with available related molecules, < 50% cross-species reactivity observed with species tested. |
| Interference | No significant interference observed with available related molecules |
| Storage/Stability | 4 ºC for up to 6 months |
| Usage | For Laboratory Research Use Only. Not for diagnostic or therapeutic use. |
| Additional Notes | The kit allows for use in multiple experiments. |
Standard Curve
Kit Components
1. Pre-coated 96-well Microplate
2. Biotinylated Detection Antibody
3. Streptavidin-HRP Conjugate
4. Lyophilized Standards
5. TMB One-Step Substrate
6. Stop Solution
7. 20 x PBS
8. Assay Buffer
Other Materials Required but not Provided:
1. Microplate Reader capable of measuring absorption at 450 nm
2. Log-log graph paper or computer and software for ELISA data analysis
3. Precision pipettes (1-1000 µl)
4. Multi-channel pipettes (300 µl)
5. Distilled or deionized water
Protocol Outline
1. Prepare all reagents, samples and standards as instructed in the datasheet.
2. Add 100 µl of Standard or samples to each well and incubate 1 h at RT.
3. Add 100 µl of Working Detection Antibody to each well and incubate 1 h at RT.
4. Add 100 µl of Working Streptavidin-HRP to each well and incubate 20 min at RT.
5. Add 100 µl of Substrate to each well and incubate 5-30 min at RT.
6. Add 50 µl of Stop Solution to each well and read at 450 nm immediately.
Background:
Interleukin 9 (IL-9) is a pleiotropic cytokine (cell signalling molecule) belonging to the group of interleukins.[1] IL-9 is produced by variety of cells like mast cells, NKT cells, Th2, Th17, Treg, ILC2, and Th9 cells in different amounts. Among them, Th9 cells are regarded as the major CD4+ T cells that produce IL-9. Il-9 is secreted by CD4+ helper cells that acts as a regulator of a variety of hematopoietic cells.[2] Interleukin 33 (IL-33) induces IL-9 expression and secretion in T cells, which was confirmed by the results obtained in mice by using Human in vitro system.[3] Whereas the report of others confirms that TGF-β is an essential factor for IL-9 induction.[4] For the first time (Lars Blom,Britta C. Poulsen,Bettina M. Jensen,Anker Hansen and Lars K. Poulsen published a journal online in 2011 Jul 6),indicating that TGF-β may be important for production of IL-9 but it is not only the definite requirement for IL-9 induction, since cultures with IL-33 without TGF-β have noticeably increased secretion of IL-9, suggesting an important role of IL-33, even though that the effect was not found significant on the gene level.[5] This cytokine stimulates cell proliferation and prevents apoptosis. It functions through the interleukin-9 receptor (IL9R), which activates different signal transducer and activator (STAT) proteins namely STAT1, STAT3 and STAT5 and thus connects this cytokine to various biological processes. [6] The gene encoding this cytokine has been identified as a candidate gene for asthma. Genetic studies on a mouse model of asthma demonstrated that this cytokine is a determining factor in the pathogenesis of bronchial hyperresponsiveness. Interleukin-9 has also shown to inhibit melanoma growth in mice.[7] Additionally, it gives rise to the multiplication of hematologic neoplasias and also Hodgkin’s lymphoma in humans but IL-9 also has antitumor properties in solid tumors, for example melanoma.
References
- Kelleher K, et al. (1991). Blood. 77 (7): 1436–41.
- Perumal NB, Kaplan MH (2011). Trends in Immunology. 32 (4): 146–50.
- Humphreys NE, et al. (2008). Journal of Immunology. 180 (4): 2443–9.
- Beriou G, et al. (2010). Journal of Immunology. 185 (1): 46–54.
- Blom L, et al. (2011). PLoS One. 6 (7): e21695. doi:1371/journal.pone.0021695.
- Purwar R, et al. (2012). Nature Medicine. 18 (8): 1248–53.
- Knoops L, Renauld JC (2004). Growth Factors. 22 (4): 207–15.
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