Nori Human GAS6 ELISA Kit
$461.00 – $832.00
This ELISA kit is for quantification of GAS6 in human. This is a quick ELISA assay that reduces time to 50% compared to the conventional method, and the entire assay only takes 3 hours. This assay employs the quantitative sandwich enzyme immunoassay technique and uses biotin-streptavidin chemistry to improve the performance of the assays. An antibody specific for GAS6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GAS6 present is bound by the immobilized antibody. After washing away any unbound substances, a detection antibody specific for GAS6 is added to the wells. Following wash to remove any unbound antibody reagent, a detection reagent is added. After intensive wash a substrate solution is added to the wells and color develops in proportion to the amount of GAS6 bound in the initial step. The color development is stopped, and the intensity of the color is measured.
Alternative names for GAS6: Growth arrest-specific 6, AXLLG
This product is for laboratory research use only, not for diagnostic and therapeutic purposes or any other purposes.
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Description
Nori Human GAS6 ELISA Kit Summary
Alternative names for GAS6: Growth arrest-specific 6, AXLLG
| Assay Type | Solid Phase Sandwich ELISA |
| Format | 96-well Microplate or 96-Well Strip Microplate |
| Method of Detection | Colorimetric |
| Number of Targets Detected | 1 |
| Target Antigen Accession Number | Q14393 |
| Assay Length | 3 hours |
| Quantitative/Semiquantitative | Quantitative |
| Sample Type | Plasma, Serum, Cell Culture, Urine, Cell/Tissue Lysates, Synovial Fluid, BAL, |
| Recommended Sample Dilution (Plasma/Serum) | No dilution for sample <ULOQ; sufficient dilution for samples >ULOQ |
| Sensitivity | 6 pg/mL |
| Detection Range | 31.25-2000 pg/mL |
| Specificity | Human GAS6 |
| Cross-Reactivity | < 0.5% cross-reactivity observed with available related molecules, < 50% cross-species reactivity observed with species tested. |
| Interference | No significant interference observed with available related molecules |
| Storage/Stability | 4 ºC for up to 6 months |
| Usage | For Laboratory Research Use Only. Not for diagnostic or therapeutic use. |
| Additional Notes | The kit allows for use in multiple experiments. |
Standard Curve
Kit Components
1. Pre-coated 96-well Microplate
2. Biotinylated Detection Antibody
3. Streptavidin-HRP Conjugate
4. Lyophilized Standards
5. TMB One-Step Substrate
6. Stop Solution
7. 20 x PBS
8. Assay Buffer
Other Materials Required but not Provided:
1. Microplate Reader capable of measuring absorption at 450 nm
2. Log-log graph paper or computer and software for ELISA data analysis
3. Precision pipettes (1-1000 µl)
4. Multi-channel pipettes (300 µl)
5. Distilled or deionized water
Protocol Outline
1. Prepare all reagents, samples and standards as instructed in the datasheet.
2. Add 100 µl of Standard or samples to each well and incubate 1 h at RT.
3. Add 100 µl of Working Detection Antibody to each well and incubate 1 h at RT.
4. Add 100 µl of Working Streptavidin-HRP to each well and incubate 20 min at RT.
5. Add 100 µl of Substrate to each well and incubate 5-30 min at RT.
6. Add 50 µl of Stop Solution to each well and read at 450 nm immediately.
Background:
Growth arrest-specific 6 (GAS6) is a gene coding for the Gas6 protein. [1] GAS6 is a member of the vitamin K-dependent proteins related to protein S, a negative coregulator in the blood coagulation cascade. [2] It is similar to the Protein S with the same domain organization and 43% amino acid identity. It was originally found as a gene upregulated by growth arrested fibroblasts. Gas6 is a gamma-carboxyglutamic acid (Gla) domain-containing protein thought to be involved in the stimulation of cell proliferation. The presence of Gla needs a vitamin K-dependent enzymatic reaction that carboxylates the gamma carbon of certain glutamic residues of the protein during its production in the endoplasmic reticulum. Gas6 has been shown to interact with AXL receptor tyrosine kinase, MerTK and TYRO3.[1][3] GAS6 modulates platelet thrombus formation and vascular wall homeostasis and represents an attractive drug target.[4] GAS6 is also identified as a growth factor.[5] GAS6 has mitogenic and survival activities for serum starved NIH3T3 fibroblasts.[6] The growth arrest-specific gene product Gas6 promotes the survival of human oligodendrocytes via a phosphatidylinositol 3-kinase-dependent pathway.[7]
References
- Mark MR, et al. (1996J. Biol. Chem. 271 (16): 9785–9.
- Manfioletti G, et al. (1993). Mol. Cell. Biol. 13 (8): 4976–85.
- Nagata K, et al. (1996). J. Biol. Chem. 271 (47): 30022–7.
- Maree AO, et al. (2007). Curr. Pharm. Des. 13 (26): 2656–61.
- Li R, et al. (1996). J. Neurosci. 16 (6): 2012–9.
- Goruppi S, et al. (1996). Oncogene. 12 (3): 471–80.
- Shankar SL, et al. (2003). J. Neurosci. 23 (10): 4208–18.
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