Nori Human Collagen II ELISA Kit
$461.00 – $832.00
This ELISA kit is for quantification of Collagen II in human. This is a quick ELISA assay that reduces time to 50% compared to the conventional method, and the entire assay only takes 3 hours. This assay employs the quantitative sandwich enzyme immunoassay technique and uses biotin-streptavidin chemistry to improve the performance of the assays. An antibody specific for COL2A1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any COL2A1 present is bound by the immobilized antibody. After washing away any unbound substances, a detection antibody specific for COL2A1 is added to the wells. Following wash to remove any unbound antibody reagent, a detection reagent is added. After intensive wash a substrate solution is added to the wells and color develops in proportion to the amount of COL2A1 bound in the initial step. The color development is stopped, and the intensity of the color is measured.
Alternative names for collagen II: COL2A1, type II collagen
This product is for laboratory research use only not for diagnostic and therapeutic purposes or any other purposes.
- Description
- How Elisa Works
- Product Citations
- Reviews (0)
Description
Nori Human Collagen II ELISA Kit Summary
Alternative names for collagen II: COL2A1, type II collagen
| Assay Type | Solid Phase Sandwich ELISA |
| Format | 96-well Microplate or 96-Well Strip Microplate |
| Method of Detection | Colorimetric |
| Number of Targets Detected | 1 |
| Target Antigen Accession Number | P02458 |
| Assay Length | 3 hours |
| Quantitative/Semiquantitative | Quantitative |
| Sample Type | Plasma, Serum, Cell Culture, Urine, Cell/Tissue Lysates, Synovial Fluid, BAL, |
| Recommended Sample Dilution (Plasma/Serum) | No dilution for sample <ULOQ; sufficient dilution for samples >ULOQ |
| Sensitivity | 30 pg/mL |
| Detection Range | 156-10,000 pg/mL |
| Specificity | Human COL2A1 |
| Cross-Reactivity | < 0.5% cross-reactivity observed with available related molecules, < 50% cross-species reactivity observed with species tested. |
| Interference | No significant interference observed with available related molecules |
| Storage/Stability | 4 ºC for up to 6 months |
| Usage | For Laboratory Research Use Only. Not for diagnostic or therapeutic use. |
| Additional Notes | The kit allows for use in multiple experiments. |
Standard Curve
Kit Components
1. Pre-coated 96-well Microplate
2. Biotinylated Detection Antibody
3. Streptavidin-HRP Conjugate
4. Lyophilized Standards
5. TMB One-Step Substrate
6. Stop Solution
7. 20 x PBS
8. Assay Buffer
Other Materials Required but not Provided:
1. Microplate Reader capable of measuring absorption at 450 nm
2. Log-log graph paper or computer and software for ELISA data analysis
3. Precision pipettes (1-1000 µl)
4. Multi-channel pipettes (300 µl)
5. Distilled or deionized water
Protocol Outline
1. Prepare all reagents, samples and standards as instructed in the datasheet.
2. Add 100 µl of Standard or samples to each well and incubate 1 h at RT.
3. Add 100 µl of Working Detection Antibody to each well and incubate 1 h at RT.
4. Add 100 µl of Working Streptavidin-HRP to each well and incubate 20 min at RT.
5. Add 100 µl of Substrate to each well and incubate 5-30 min at RT.
6. Add 50 µl of Stop Solution to each well and read at 450 nm immediately.
Background:
Type II collagen (Collagen II) is encoded by COL2A1 gene.[1] Collagen II is the basis for articular cartilage and hyaline cartilage. It makes up 50% of all protein in cartilage and 85-90% of collagen of articular cartilage. Type II collagen does form fibrils. This fibrillar network of collagen allows cartilage to entrap the proteoglycan aggregate as well as provide tensile strength to the tissue. Oral administration of native collagen type II induces oral tolerance to pathological immune responses and may be useful in arthritis.[2][3]
References
- Baldwin,C.T., et al. (1989) Structure of cDNA clones coding for human type II procollagen. The
- alpha 1(II) chain is more similar to the alpha 1(I) chain than two alpha chains of fibrillar collagens. Biochem. J. 262 (2), 521-528.
- Park, Kyung-Su; et al. (2009). “Type II collagen oral tolerance; mechanism and role in collagen-induced arthritis and rheumatoid arthritis”. Modern Rheumatology. 19 (6): 581–589.
- Bakilan, Fulya; et al. (2016). “Effects of Native Type II Collagen Treatment on Knee Osteoarthritis: A Randomized Controlled Trial”. The Eurasian Journal of Medicine. 48 (2): 95–101.
Product Citations
Be the first to review “Nori Human Collagen II ELISA Kit”
You must be logged in to post a review.
Reviews
There are no reviews yet.