Description

Nori Human Cardiotrophin-1 ELISA Kit Summary

Alternative names for cardiotrophin-1: CT-1, CTF1

Standard Curve

Kit Components
1. Pre-coated 96-well Microplate
2. Biotinylated Detection Antibody
3. Streptavidin-HRP Conjugate
4. Lyophilized Standards
5. TMB One-Step Substrate
6. Stop Solution
7. 20 x PBS
8. Assay Buffer

Other Materials Required but not Provided:
1. Microplate Reader capable of measuring absorption at 450 nm
2. Log-log graph paper or computer and software for ELISA data analysis
3. Precision pipettes (1-1000 µl)
4. Multi-channel pipettes (300 µl)
5. Distilled or deionized water

Protocol Outline
1. Prepare all reagents, samples and standards as instructed in the datasheet.
2. Add 100 µl of Standard or samples to each well and incubate 1 h at RT.
3. Add 100 µl of Working Detection Antibody to each well and incubate 1 h at RT.
4. Add 100 µl of Working Streptavidin-HRP to each well and incubate 20 min at RT.
5. Add 100 µl of Substrate to each well and incubate 5-30 min at RT.
6. Add 50 µl of Stop Solution to each well and read at 450 nm immediately.

Background: 

Cardiotrophin-1 (CT-1) is a cytokine and a cardiac hypertrophic factor of 21.5 kDa and a protein member of the IL-6 cytokine family.[1] CT-1 is associated with the pathophysiology of heart diseases, including hypertension, myocardial infarction, valvular heart disease, and congestive heart failure.[2] The protein exerts its cellular effects by interacting with the glycoprotein 130 (gp130)/leukemia inhibitory factor receptor beta (LIFR) heterodimer.[2] In addition, CT-1 activates phosphatidylinositol 3-kinase in cardiac myocytes and enhances transcription factor NF-κB DNA -binding activities. CT-1 is highly expressed in the heart, skeletal muscle, prostate and ovary and to lower levels in lung, kidney, pancreas, thymus, testis and small intestine. Non-diabetic subjects who were overweight or obesity had significantly lower cardiotrophin-1 concentrations than those with normal weight, and both obesity and being overweight were inversely associated with cardiotrophin-1 levels.[4] A transcriptional factor, Myc-associated zinc finger protein (MAZ), plays an important role in ADAM10 transcription in response to CT-1 in neural stem/progenitor cells.[5] Cardiotrophin-1 levels were not an independent predictor of all-cause mortality in hemodialysis patients.[6] The cytokine cardiotrophin 1 (CT1) acts as a factor capable of recapitulating the key features of physiologic growth of the heart including transient and reversible hypertrophy of the myocardium, and stimulation of cardiomyocyte-derived angiogenic signals leading to increased vascularity.[7] In children with pediatric obesity, lifestyle weight-loss intervention results in down-regulation of serum cardiotrophin-1 (CTF1), interleukin-6 (IL6), and tumor necrosis factor-alpha (TNFA); expression of CTF1, IL6, and TNFA is also down-regulated in peripheral blood mononuclear cells after improvement in adiposity, body mass index, and waist-hip ratio.[8]

References

1. Pennica D, et al. (1995) Proc. Natl. Acad. Sci. U.S.A. 92 (4), 1142-1146.
2. Erdmann J, et al. (2000) Hum. Mutat. 16 (5), 448.
3. Pennica D, et al. (1995) J. Biol. Chem. 270 (18), 10915-10922.
4. Hung HC, et al. (2015) Sci Rep 5, 17438.
5. Liu B, et al. (2016) Sci Rep 6, 21534.
6. Hogas S, et al. (2016) Angiology 67 (9), 854-859.
7. Abdul-Ghani M, et al. (2017) Cell Res. 27 (10), 1195-1215.
8. Marti A, et al. (2018) Pediatr Diabetes 19 (2), 217-222.

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