Nori Feline Angiopoietin-like 4 (ANGPTL4) ELISA Kit
$508.00 – $916.00
This ELISA kit is for quantification of ANGPTL4 in feline. This is a quick ELISA assay that reduces time to 50% compared to the conventional method, and the entire assay only takes 3 hours. This assay employs the quantitative sandwich enzyme immunoassay technique and uses biotin-streptavidin chemistry to improve the performance of the assays. An antibody specific for VEGFR1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ANGPTL4 present is bound by the immobilized antibody. After washing away any unbound substances, a detection antibody specific for ANGPTL4 is added to the wells. Following wash to remove any unbound antibody reagent, a detection reagent is added. After intensive wash a substrate solution is added to the wells and color develops in proportion to the amount of ANGPTL4 bound in the initial step. The color development is stopped, and the intensity of the color is measured.
Alternative names for Angiopoietin-Like 4: ANGPTL4
This product is for laboratory research use only not for diagnostic and therapeutic purposes or any other purposes.
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Description
Nori Feline Angiopoietin-like 4 (ANGPTL4) ELISA Kit Summary
Alternative names for Angiopoietin-Like 4: ANGPTL4
Alternative names for feline: cat
| Assay Type | Solid Phase Sandwich ELISA |
| Format | 96-well Microplate or 96-Well Strip Microplate |
| Method of Detection | Colorimetric |
| Number of Targets Detected | 1 |
| Target Antigen Accession Number |
A0ABI7ZN63 |
| Assay Length | 3 hours |
| Quantitative/Semiquantitative | Quantitative |
| Sample Type | Plasma, Serum, Cell Culture, Urine, Cell/Tissue Lysates, Synovial Fluid, BAL, |
| Recommended Sample Dilution (Plasma/Serum) | No dilution for sample <ULOQ; sufficient dilution for samples >ULOQ |
| Sensitivity | 50 pg/mL |
| Detection Range | 0.25-16 ng/mL |
| Specificity | Feline ANGPTL4 |
| Cross-Reactivity | < 0.5% cross-reactivity observed with available related molecules, < 50% cross-species reactivity observed with species tested. |
| Interference | No significant interference observed with available related molecules |
| Storage/Stability | 4 ºC for up to 6 months |
| Usage | For Laboratory Research Use Only. Not for diagnostic or therapeutic use. |
| Additional Notes | The kit allows for use in multiple experiments. |
Standard Curve
Kit Components
1. Pre-coated 96-well Microplate
2. Biotinylated Detection Antibody
3. Streptavidin-HRP Conjugate
4. Lyophilized Standards
5. TMB One-Step Substrate
6. Stop Solution
7. 20 x PBS
8. Assay Buffer
Other Materials Required but not Provided:
1. Microplate Reader capable of measuring absorption at 450 nm
2. Log-log graph paper or computer and software for ELISA data analysis
3. Precision pipettes (1-1000 µl)
4. Multi-channel pipettes (300 µl)
5. Distilled or deionized water
Protocol Outline
1. Prepare all reagents, samples and standards as instructed in the datasheet.
2. Add 100 µl of Standard or samples to each well and incubate 1 h at RT.
3. Add 100 µl of Working Detection Antibody to each well and incubate 1 h at RT.
4. Add 100 µl of Working Streptavidin-HRP to each well and incubate 20 min at RT.
5. Add 100 µl of Substrate to each well and incubate 5-30 min at RT.
6. Add 50 µl of Stop Solution to each well and read at 450 nm immediately.
Background:
VEGFR1 (Vascular Endothelial Growth Factor Receptor-1), also known as Flt1 (fms-like tyrosine kinase 1) is a protein that is encoded by the FLT1 gene.[1], and is a receptor tyrosine kinase that plays a critical role in angiogenesis and vasculogenesis. It binds ligands such as VEGF-A, VEGF-B, and placental growth factor (PlGF) with high affinity, often acting as a “decoy” receptor to regulate vascular development by limiting VEGF-A availability for the more active VEGFR-2. FLT1 is a member of VEGF receptor gene family. It encodes a receptor tyrosine kinase which is activated by VEGF-A, VEGF-B, and PIGF. The sequence structure of the FLT1 gene resembles that of the FMS (now CSF1R) gene. The ablation of VEGFR1 by chemical and genetic means has also recently been found to augment the conversion of white adipose tissue to brown adipose tissue as well as increase brown adipose angiogenesis in mice.[2] Functional genetic variation in FLT1 (rs9582036) has been found to affect non-small cell lung cancer survival.[3] FLT1 has been shown to interact with PLCG1[4] and VEGF-B.[5]
References
- Shibuya M, et al. (1990). Oncogene. 5 (4): 519–24.
- Seki T, et al. (2018). The Journal of Experimental Medicine. 215 (2): 611–626.
- Glubb DM, et al. (2015). Journal of Thoracic Oncology. 10 (7): 1067–75.
- Cunningham SA, et al. (1997). Biochemical and Biophysical Research Communications. 240 (3): 635–9.
- Olofsson B, et al. (1998). Proceedings of the National Academy of Sciences of the United States of America. 95 (20): 11709–14.
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