Description

Nori Equine BMP6 ELISA Kit Summary

Alternative names for BMP6: Bone morphogenetic protein 6, VGR

Alternative names for equine: horse

Standard Curve

Kit Components
1. Pre-coated 96-well Microplate
2. Biotinylated Detection Antibody
3. Streptavidin-HRP Conjugate
4. Lyophilized Standards
5. TMB One-Step Substrate
6. Stop Solution
7. 20 x PBS
8. Assay Buffer

Other Materials Required but not Provided:
1. Microplate Reader capable of measuring absorption at 450 nm
2. Log-log graph paper or computer and software for ELISA data analysis
3. Precision pipettes (1-1000 µl)
4. Multi-channel pipettes (300 µl)
5. Distilled or deionized water

Protocol Outline
1. Prepare all reagents, samples and standards as instructed in the datasheet.
2. Add 100 µl of Standard or samples to each well and incubate 1 h at RT.
3. Add 100 µl of Working Detection Antibody to each well and incubate 1 h at RT.
4. Add 100 µl of Working Streptavidin-HRP to each well and incubate 20 min at RT.
5. Add 100 µl of Substrate to each well and incubate 5-30 min at RT.
6. Add 50 µl of Stop Solution to each well and read at 450 nm immediately.

Background: 

Bone morphogenetic protein 6 (BMP6) is a protein that in humans is encoded by the BMP6 gene.^[1][2] The protein encoded by this gene is a member of the TGFβ superfamily. Bone morphogenetic proteins (BMPs) are known for their ability to induce the growth of bone and cartilage. BMP6 is able to induce all osteogenic markers in mesenchymal stem cells. The BMPs are a family of secreted signaling molecules that can induce ectopic bone growth. BMPs are part of the transforming growth factor-beta (TGFB) superfamily. BMPs were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extra skeletal site. Based on its expression early in embryogenesis, the BMP encoded by this gene has a proposed role in early development. In addition, the fact that this BMP is closely related to BMP5 and BMP7 has led to speculation of possible bone inductive activity. As of April 2009, an additional function of BMP6 has been identified as described in Nature Genetics April; 41 [4]:386-8. BMP6 is the key regulator of hepcidin, the small peptide secreted by the liver which is the major regulator of iron metabolism in mammals. BMP6 is expressed in normal and atherosclerotic smooth muscle cells,[3] normal and malignant prostatic tissue,[4] healthy and osteoarthritic human articular chondrocytes and can stimulate matrix synthesis in vitro.[5] Bone morphogenetic proteins 2 and 6, expressed in arthritic synovium, are regulated by proinflammatory cytokines and differentially modulate fibroblast-like synoviocyte apoptosis.[6]

References

1. Hahn GV, et al. (1992) Genomics. 14(3): 759–62.
2. Sauermann U, et al. (1993). J Neurosci Res. 33(1): 142–7.
3. Schluesener HJ, Meyermann R (1995). Atherosclerosis. 113(2): 153–6.
4. Hamdy FC, et al. (1997). Cancer Res. 57(19): 4427–31.
5. Bobacz K, et al. (2003). Arthritis Rheum. 48(9): 2501–8.
6. Lories RJ, et al. (2003). Arthritis Rheum. 48(10): 2807–18.

Product Citations