Nori Equine Angiopoietin 1 ELISA Kit
$461.00 – $832.00
This ELISA kit is for quantification of ANG-1 in horse. This is a quick ELISA assay that reduces time to 50% compared to the conventional method, and the entire assay only takes 3 hours. This assay employs the quantitative sandwich enzyme immunoassay technique and uses biotin-streptavidin chemistry to improve the performance of the assays. An antibody specific for ANG-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ANG-1 present is bound by the immobilized antibody. After washing away any unbound substances, a detection antibody specific for ANG-1 is added to the wells. Following wash to remove any unbound antibody reagent, a detection reagent is added. After intensive wash a substrate solution is added to the wells and color develops in proportion to the amount of ANG-1 bound in the initial step. The color development is stopped, and the intensity of the color is measured.
Alternative names for angipoietin 1: ANG-1, ANGPT1, ANG1
This product is for laboratory research use only not for diagnostic and therapeutic purposes or any other purposes.
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Description
Nori Equine Angipoietin 1 ELISA Kit Summary
Alternative names for angipoietin 1: ANG-1, ANGPT1, ANG1
Alternative name for equine: Horse
| Assay Type | Solid Phase Sandwich ELISA |
| Format | 96-well Microplate or 96-Well Strip Microplate |
| Method of Detection | Colorimetric |
| Number of Targets Detected | 1 |
| Target Antigen Accession Number | F6XYS3 |
| Assay Length | 3 hours |
| Quantitative/Semiquantitative | Quantitative |
| Sample Type | Plasma, Serum, Cell Culture, Urine, Cell/Tissue Lysates, Synovial Fluid, BAL, |
| Recommended Sample Dilution (Plasma/Serum) | No dilution for sample <ULOQ; sufficient dilution for samples >ULOQ |
| Sensitivity | 15 pg/mL |
| Detection Range | 78.13-5000 pg/mL |
| Specificity | Equine ANG-1 |
| Cross-Reactivity | < 0.5% cross-reactivity observed with available related molecules, < 50% cross-species reactivity observed with species tested. |
| Interference | No significant interference observed with available related molecules |
| Storage/Stability | 4 ºC for up to 6 months |
| Usage | For Laboratory Research Use Only. Not for diagnostic or therapeutic use. |
| Additional Notes | The kit allows for use in multiple experiments. |
Standard Curve
Kit Components
1. Pre-coated 96-well Microplate
2. Biotinylated Detection Antibody
3. Streptavidin-HRP Conjugate
4. Lyophilized Standards
5. TMB One-Step Substrate
6. Stop Solution
7. 20 x PBS
8. Assay Buffer
Other Materials Required but not Provided:
1. Microplate Reader capable of measuring absorption at 450 nm
2. Log-log graph paper or computer and software for ELISA data analysis
3. Precision pipettes (1-1000 µl)
4. Multi-channel pipettes (300 µl)
5. Distilled or deionized water
Protocol Outline
1. Prepare all reagents, samples and standards as instructed in the datasheet.
2. Add 100 µl of Standard or samples to each well and incubate 1 h at RT.
3. Add 100 µl of Working Detection Antibody to each well and incubate 1 h at RT.
4. Add 100 µl of Working Streptavidin-HRP to each well and incubate 20 min at RT.
5. Add 100 µl of Substrate to each well and incubate 5-30 min at RT.
6. Add 50 µl of Stop Solution to each well and read at 450 nm immediately.
Background:
Angiopoietin 1 (ANG-1) is a type of angiopoietin and is encoded by the gene ANGPT1. [1] Angiopoietins are proteins with important roles in vascular development and angiogenesis. All angiopoietins bind with similar affinity to an endothelial cell-specific tyrosine-protein kinase receptor.[2] The protein encoded by this gene is a secreted glycoprotein that activates the receptor by inducing its tyrosine phosphorylation. It plays a critical role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme. The protein also contributes to blood vessel maturation and stability, and may be involved in early development of the heart.[1] Angiopoietin 1 has been shown to interact with TEK tyrosine kinase. [3][4]
References
- Davis S, et al. (1996). “Isolation of angiopoietin-1, a ligand for the TIE2 receptor, by secretion-trap expression cloning”.Cell (United States) 87 (7): 1161–9.
- Maisonpierre, P C; et al. (1997). “Angiopoietin-2, a natural antagonist for Tie2 that disrupts in vivo angiogenesis”.Science (UNITED STATES)277 (5322): 55–60.
- Fiedler U, et al. (2003). “Angiopoietin-1 and angiopoietin-2 share the same binding domains in the Tie-2 receptor involving the first Ig-like loop and the epidermal growth factor-like repeats”. Biol. Chem. (United States) 278 (3): 1721–7.
- Sato A, et al. (1998). “Characterization of TEK receptor tyrosine kinase and its ligands, Angiopoietins, in equine hematopoietic progenitor cells”. Immunol. (ENGLAND) 10 (8): 1217–27.
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