Nori Bovine BMP3 ELISA Kit
$461.00 – $832.00
This ELISA kit is for quantification of BMP3 in bovine. This is a quick ELISA assay that reduces time to 50% compared to the conventional method, and the entire assay only takes 3 hours. This assay employs the quantitative sandwich enzyme immunoassay technique and uses biotin-streptavidin chemistry to improve the performance of the assays. An antibody specific for BMP3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any BMP3 present is bound by the immobilized antibody. After washing away any unbound substances, a detection antibody specific for BMP3 is added to the wells. Following wash to remove any unbound antibody reagent, a detection reagent is added. After intensive wash a substrate solution is added to the wells and color develops in proportion to the amount of BMP3 bound in the initial step. The color development is stopped, and the intensity of the color is measured.
Alternative names for BMP3: Bone morphogenetic protein 3 (BMP3)
This product is for Laboratory Research Use Only not for diagnostic and therapeutic purposes or any other purposes.
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Description
Nori Bovine BMP3 ELISA Kit Summary
Alternative names for BMP3: Bone morphogenetic protein 3 (BMP3)
Alternative names for bovine: cattle, cow, bull
| Assay Type | Solid Phase Sandwich ELISA |
| Format | 96-well Microplate or 96-Well Strip Microplate |
| Method of Detection | Colorimetric |
| Number of Targets Detected | 1 |
| Target Antiben Accession Number | P22444 |
| Assay Length | 3 hours |
| Quantitative/Semiquantitative | Quantitative |
| Sample Type | Plasma, Serum, Cell Culture, Urine, Cell/Tissue Lysates, Synovial Fluid, BAL, |
| Recommended Sample Dilution (Plasma/Serum) | No dilution for sample <ULOQ; sufficient dilution for samples >ULOQ |
| Sensitivity | 5 pg/mL |
| Detection Range | 25-1600 pg/mL |
| Specificity | Bovine BMP3 |
| Cross-Reactivity | < 0.5% cross-reactivity observed with available related molecules, < 50% cross-species reactivity observed with species tested. |
| Interference | No significant interference observed with available related molecules |
| Storage/Stability | 4 ºC for up to 6 months |
| Usage | For Laboratory Research Use Only. Not for diagnostic or therapeutic use. |
| Additional Notes | The kit allows for use in multiple experiments. |
Standard Curve
Kit Components
1. Pre-coated 96-well Microplate
2. Biotinylated Detection Antibody
3. Streptavidin-HRP Conjugate
4. Lyophilized Standards
5. TMB One-Step Substrate
6. Stop Solution
7. 20 x PBS
8. Assay Buffer
Other Materials Required but not Provided:
1. Microplate Reader capable of measuring absorption at 450 nm
2. Log-log graph paper or computer and software for ELISA data analysis
3. Precision pipettes (1-1000 µl)
4. Multi-channel pipettes (300 µl)
5. Distilled or deionized water
Protocol Outline
1. Prepare all reagents, samples and standards as instructed in the datasheet.
2. Add 100 µl of Standard or samples to each well and incubate 1 h at RT.
3. Add 100 µl of Working Detection Antibody to each well and incubate 1 h at RT.
4. Add 100 µl of Working Streptavidin-HRP to each well and incubate 20 min at RT.
5. Add 100 µl of Substrate to each well and incubate 5-30 min at RT.
6. Add 50 µl of Stop Solution to each well and read at 450 nm immediately.
Background:
Bone morphogenetic protein 3 (BMP3) is a protein in humans that is encoded by the BMP3 gene.[1] The protein encoded by this gene is a member of the transforming growth factor beta superfamily. It, like other bone morphogenetic proteins (BMP’s) is known for its ability to induce bone and cartilage development. It is a disulfide-linked homodimer. It negatively regulates bone density.[2] BMP3 is an antagonist to other BMP’s in the differentiation of osteogenic progenitors. BMP3 suppresses osteoblast differentiation of bone marrow stromal cells via interaction with activin receptor type 2b.[3] It is highly expressed in fractured tissues.[4] BMP3 is downregulated in cholangiocarcinoma (CC) and may act as a biomarker of the cancer.[5] Furthermore, promotor region methylation of the BMP3 gene may cause gastric carcinoma.[6]
References
- Faucheux C, et al. (1998). Biophys. Res. Commun. 241 (3): 787–93.
- Daluiski A, et al. (2001). Genet. 27 (1): 84–8.
- Kokabu S1, (2012) Mol Endocrinol 26:87-94.
- Kloen P, Di Paola M, Borens O et al. (2004). Bone 33 (3): 362–71.
- Kisiel JB1, (2013) J Mol Biomark Diagn. 4(145):1000145.
- Chen XR, et al. (2010) World J Gastroenterol. 2010 Mar 21;16(11):1409-13.
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